Assembly of the precursor and processed light-harvesting chlorophyll a/b protein of Lemna into the light-harvesting complex II of barley etiochloroplasts.

When the in vitro synthesized precursor of a light-harvesting chlorophyll a/b binding protein (LHCP) from Lemna gibba is imported into barley etiochloroplasts, it is processed to a single form. Both the processed form and the precursor are found in the thylakoid membranes, assembled into the light-harvesting complex of photosystem II. Neither form can be detected in the stromal fraction. The relative amounts of precursor and processed forms observed in the thylakoids are dependent on the developmental stage of the plastids used for uptake. The precursor as well as the processed form can also be detected in thylakoids of greening maize plastids used in similar uptake experiments. This detection of a precursor in the thylakoids, which has not been previously reported, could be a result of using rapidly developing plastids and/or using an heterologous system. Our results demonstrate that the extent of processing of LHCP precursor is not a prerequisite for its inclusion in the complex. They are also consistent with the possibility that the processing step can occur after insertion of the protein into the thylakoid membrane

Precursor of Inflation

We investigate a nonsingular initial state of the Universe which leads to inflation naturally. The model is described by a scalar field with a quadratic potential in Eddington-inspired Born-Infeld gravity. The curvature of this initial state is given by the mass scale of the scalar field which is much smaller than the Planck scale. Therefore, in this model, quantum gravity is not necessary in understanding this pre-inflationary stage, no matter how large the energy density becomes. The initial state in this model evolves eventually to a long inflationary period which is similar to the usual chaotic inflation.Comment: 6pages, 1figur

Export of honeybee prepromelittin in Escherichia coli depends on the membrane potential but does not depend on proteins secA and secY

Honeybee prepromelittin (70 amino acid residues), the precursor of an eukaryotic secretory protein, and a hybrid protein between prepromelittin and mouse dihydrofolate reductase (257 amino acid residues) were expressed in Escherichia coli and characterized with respect to their requirements for transport across the plasma membrane. Both precursor proteins are posttranslationally processed and exported into the periplasm, and they both depend on the membrane potential for this to occur. With respect to dependence on components of the export machinery, however, the two precursor proteins show striking differences: the small precursor protein prepromelittin does not require the function of proteins secA and secY; the large precursor protein prepromelittin-dihydrofolate reductase, on the other hand, depends on both components. The implications of these observations with respect to the mechanisms of protein export in E. coli and of protein import into the endoplasmic reticulum are discussed

Precursor of Color Superconductivity

We investigate possible precursory phenomena of color superconductivity at finite temperature $T$ with an effective theory of QCD. It is found that the fluctuation of the diquark pair field exists with a prominent strength even well above the critical temperature $T_c$. We show that such a fluctuaiton forms a collective mode, the corresponding pole of which approaches the origin as $T$ is lowered to $T_c$ in the complex energy plane. We discuss the possible relevance of the precursor to the observables to be detected in heavy-ion collisions.Comment: 4 pages, 5 figures, Talk presented at the XVIth International Conference on Particles and Nuclei (PANIC02), Osaka, Japan, Sep.30 - Oct.4, 2002, Uses espcrc1.st

Precursor flares in OJ 287

We have studied three most recent precursor flares in the light curve of the blazar OJ 287 while invoking the presence of a precessing binary black hole in the system to explain the nature of these flares. Precursor flare timings from the historical light curves are compared with theoretical predictions from our model that incorporate effects of an accretion disk and post-Newtonian description for the binary black hole orbit. We find that the precursor flares coincide with the secondary black hole descending towards the accretion disk of the primary black hole from the observed side, with a mean z-component of approximately z_c = 4000 AU. We use this model of precursor flares to predict that precursor flare of similar nature should happen around 2020.96 before the next major outburst in 2022.Comment: to appear in the Astrophysical Journa

Precursor films in wetting phenomena

The spontaneous spreading of non-volatile liquid droplets on solid substrates poses a classic problem in the context of wetting phenomena. It is well known that the spreading of a macroscopic droplet is in many cases accompanied by a thin film of macroscopic lateral extent, the so-called precursor film, which emanates from the three-phase contact line region and spreads ahead of the latter with a much higher speed. Such films have been usually associated with liquid-on-solid systems, but in the last decade similar films have been reported to occur in solid-on-solid systems. While the situations in which the thickness of such films is of mesoscopic size are rather well understood, an intriguing and yet to be fully understood aspect is the spreading of microscopic, i.e., molecularly thin films. Here we review the available experimental observations of such films in various liquid-on-solid and solid-on-solid systems, as well as the corresponding theoretical models and studies aimed at understanding their formation and spreading dynamics. Recent developments and perspectives for future research are discussed.Comment: 51 pages, 10 figures; small typos correcte

The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria

The precursor of the mitochondrial inner membrane protein ADP/ATP carrier is cytoplasmically synthesized without an amino-terminal peptide extension. We constructed a truncated precursor lacking the 103 amino acids from the amino terminus (about a third of the protein). Import of the truncated precursor into mitochondria showed the import characteristics of the authentic precursor, including nucleoside triphosphate dependence, requirement for a protease-sensitive component on the mitochondrial surface, two-step specific binding to the outer membrane, and membrane potential-dependent translocation into the inner membrane. We conclude that, in contrast to all other mitochondrial precursor proteins studied so far, domains of the ADP/ATP carrier distant from the amino terminus can carry specific targeting information for transport into mitochondria

Multi-wavelength Diagnostics of the Precursor and Main phases of an M1.8 Flare on 2011 April 22

We study the temporal, spatial and spectral evolution of the M1.8 flare, which occurred in NOAA AR 11195 (S17E31) on 22 April 2011, and explore the underlying physical processes during the precursors and their relation to the main phase. The study of the source morphology using the composite images in 131 {\deg}A wavelength observed by the SDO/AIA and 6-14 keV revealed a multiloop system that destabilized systematically during the precursor and main phases. In contrast, HXR emission (20-50 keV) was absent during the precursor phase, appearing only from the onset of the impulsive phase in the form of foot-points of emitting loop/s. This study has also revealed the heated loop-top prior to the loop emission, although no accompanying foot-point sources were observed during the precursor phase. We estimate the flare plasma parameters viz. T, EM, power-law index, and photon turn-over energy by forward fitting RHESSI spectral observations. The energy released in the precursor phase was thermal and constituted ~1 per cent of the total energy released during the flare. The study of morphological evolution of the filament in conjunction with synthesized T and EM maps has been carried out which reveals (a) Partial filament eruption prior to the onset of the precursor emission, (b) Heated dense plasma over the polarity inversion line and in the vicinity of the slowly rising filament during the precursor phase. Based on the implications from multi-wavelength observations, we propose a scheme to unify the energy release during the precursor and main phase emissions in which, the precursor phase emission has been originated via conduction front formed due to the partial filament eruption. Next, the heated leftover S-shaped filament has undergone slow rise and heating due to magnetic reconnection and finally erupted to produce emission during the impulsive and gradual phases.Comment: 16 Pages, 11 Figures, Accepted for Publication in MNRAS Main Journa