Exploration of methods for many-site genome editing with MAGE

Thesis (S.M.)--Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2011.Cataloged from PDF version of thesis.Includes bibliographical references (p. 28).In order to modify bacterial genetic codes, modifications must be made throughout the genome wherever the affected codon is used. Making such numerous and widespread genomic changes requires specialized techniques. MAGE is well-suited to this task, being highly amenable to multiplexing and having low time and resource costs per site. MAGE has been used as a first stage in recoding efforts, converting small clusters of sites in separate strains to be combined by other means, but improvements in MAGE technique suggest the possibility of using it to produce fully-recoded strains directly. To this end, I compare strategies based on co-selected MAGE and apply the best by performing 80 site conversions spread over 1/4 of the E coli genome.by Bram Sterling.S.M

Maine Perspective, v 10, i 15

The Maine Perspective, a publication for the University of Maine, was a campus newsletter produced by the Department of Public Affairs which eventually transformed into the Division of Marketing and Communication. Regular columns included the UM Calendar, Ongoing Events, People in Perspective, Look Who\u27s on Campus, In Focus, and Along the Mall. The weekly newsletter also included position openings on campus as well as classified ads. Included in this issue is coverage of the UMaine Black Bears winning the 1999 NCAA Hockey Championship; the creation of a Y2K awareness program on campus; the donation of $50,000 to the N. Veronica Berry Memorial Scholarship Fund; and research looking at amphibians and reptiles as early indicators of environmental change

ACUTA eNews December 1995, Vol. 24, No. 12

In This Issue From the President Cabling for the life of your network Planning today FRED fights fraud From ACUTA Headquarter

11th Annual Symposium of the School of Science, Engineering and Health

Welcome to the 11th Annual Symposium of the School of Science, Engineering and Health! This event continues a strong tradition of annual events designed to showcase student and faculty innovation, creativity and productivity in academic departments largely from within the School of Science, Engineering and Health. We look forward to incorporating new facets and improvements each year. For example, new this year: project posters will be presented by junior Engineering students during one large, multi- location, mid-afternoon poster session break that will include presentation by many other departments

11th Annual Symposium of the School of Science, Engineering and Health

Welcome to the 11th Annual Symposium of the School of Science, Engineering and Health! This event continues a strong tradition of annual events designed to showcase student and faculty innovation, creativity and productivity in academic departments largely from within the School of Science, Engineering and Health. We look forward to incorporating new facets and improvements each year. For example, new this year: project posters will be presented by junior Engineering students during one large, multi- location, mid-afternoon poster session break that will include presentation by many other departments

11th Annual Symposium of the School of Science, Engineering and Health

Welcome to the 11th Annual Symposium of the School of Science, Engineering and Health! This event continues a strong tradition of annual events designed to showcase student and faculty innovation, creativity and productivity in academic departments largely from within the School of Science, Engineering and Health. We look forward to incorporating new facets and improvements each year. For example, new this year: project posters will be presented by junior Engineering students during one large, multi- location, mid-afternoon poster session break that will include presentation by many other departments

Report of the mayor\u27s task force on consolidations and efficiencies: to Manchester board of mayor and aldermen March 15, 2010

This report discusses recommendations for improvements to the city of Manchester\u27s government, including organization and consolidation of departments, business practices, and technological advances

Strategies for Sustaining Crop Germplasm Preservation, Enhancement and Use

Issues in Agriculture no. 5 from the series "Issues in Agriculture" published by the CGIAR Secretariat