A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins

Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many proteins are assayed from single cells or other small sample sizes. However, flow-patterned arrays are hand-prepared, and so are impractical for broad applications. We describe an integrated robotics/microfluidics platform for the automated preparation of such arrays, and we apply it to the batch fabrication of up to eighteen chips of flow-patterned DNA barcodes. The resulting substrates are comparable in quality with hand-made arrays and exhibit excellent substrate-to-substrate consistency. We demonstrate the utility and reproducibility of robotics-patterned barcodes by utilizing two flow-patterned chips for highly parallel assays of a panel of secreted proteins from single macrophage cells

Writing and reading of single magnetic domain per bit perpendicular patterned media

By fabricating patterned media with a large number of nanoscale single domain magnetic particles embedded in a nonmagnetic substrate, and by writing the magnetization for each of these particles in a desired direction, nonvolatile magnetic storage of information could reach densities much higher than what is currently thought possible for longitudinal continuous media. We have fabricated high aspect ratio perpendicular nickel columnar nanoparticles embedded in a hard Al2O3/GaAs substrate. We show that the magnetization states of the individual magnets can be controlled by demonstrating that prototype patterned "single magnetic domain per bit" data tracks can be written and read back using current magnetic information storage technology

Repeated epitaxial growth and transfer of arrays of patterned, vertically aligned, crystalline Si wires from a single Si(111) substrate

Multiple arrays of Si wires were sequentially grown and transferred into a flexible polymer film from a single Si(111) wafer. After growth from a patterned, oxide-coated substrate, the wires were embedded in a polymer and then mechanically separated from the substrate, preserving the array structure in the film. The wire stubs that remained were selectively etched from the Si(111) surface to regenerate the patterned substrate. Then the growth catalyst was electrodeposited into the holes in the patterned oxide. Cycling through this set of steps allowed regrowth and polymer film transfer of several wire arrays from a single Si wafer

Nanoscale fluid flows in the vicinity of patterned surfaces

Molecular dynamics simulations of dense and rarefied fluids comprising small chain molecules in chemically patterned nano-channels predict a novel switching from Poiseuille to plug flow along the channel. We also demonstrate behavior akin to the lotus effect for a nanodrop on a chemically patterned substrate. Our results show that one can control and exploit the behavior of fluids at the nanoscale using chemical patterning.Comment: Phys. Rev. Lett. in pres

The Role of the Substrate on Pattern-Dependent Charging

Monte Carlo simulations of charging and profile evolution during plasma etching reveal that the substrate can mediate current imbalance across the wafer. This function couples patterned areas, where the electron shading effect dominates, to substrate areas directly exposed to the plasma. When a net positive current flows through the pattern features to the substrate, increasing the exposed area decreases the substrate potential, thereby causing notching at the connected feature sidewalls to worsen, in agreement with experimental observations

Effective free energy method for nematic liquid crystals in contact with structured substrates

We study the phase behavior of a nematic liquid crystal confined between a flat substrate with strong anchoring and a patterned substrate whose structure and local anchoring strength we vary. By first evaluating an effective surface free energy function characterizing the patterned substrate we derive an expression for the effective free energy of the confined nematic liquid crystal. Then we determine phase diagrams involving a homogeneous state in which the nematic director is almost uniform and a hybrid aligned nematic state in which the orientation of the director varies through the cell. Direct minimization of the free energy functional were performed in order to test the predictions of the effective free energy method. We find remarkably good agreement between the phase boundaries calculated from the two approaches. In addition the effective energy method allows one to determine the energy barriers between two states in a bistable nematic device.Comment: 10 pages, 7 figures, submitte

Solid-state dewetting on curved substrates

Based on the thermodynamic variation to the free energy functional, we propose a sharp-interface model for simulating solid-state dewetting of thin films on rigid curved substrates in two dimensions. This model describes the interface evolution which occurs through surface diffusion-controlled mass transport and contact point migration along the curved substrate. Furthermore, the surface energy anisotropy is easily included into the model, and the contact point migration is explicitly described by the relaxed contact angle boundary condition. We implement the mathematical model by a semi-implicit parametric finite element method to study several interesting phenomena, such as "small" particle migration on curved substrates and templated solid-state dewetting on a pre-patterned substrate. Based on ample numerical simulations, we demonstrate that, the migration velocity of a "small" solid particle is proportional to the substrate curvature gradient $\hat{\kappa}'$ and inversely proportional to the square root of the area of the particle $\sqrt{A}$, and it decreases when the isotropic Young angle $\theta_i$ increases. In addition, we also observe four periodic categories of dewetting on a pre-patterned sinusoidal substrate. Our approach can provide a convenient and powerful tool to exploring how to produce well-organized nanoparticles by making use of template-assisted solid-state dewetting.Comment: 14 pages, 11 figure

Image patterned molecular delivery into live cells using gold particle coated substrates.

An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated