The early expansion and evolutionary dynamics of POU class genes.

The POU genes represent a diverse class of animal-specific transcription factors that play important roles in neurogenesis, pluripotency, and cell-type specification. Although previous attempts have been made to reconstruct the evolution of the POU class, these studies have been limited by a small number of representative taxa, and a lack of sequences from basally branching organisms. In this study, we performed comparative analyses on available genomes and sequences recovered through "gene fishing" to better resolve the topology of the POU gene tree. We then used ancestral state reconstruction to map the most likely changes in amino acid evolution for the conserved domains. Our work suggests that four of the six POU families evolved before the last common ancestor of living animals-doubling previous estimates-and were followed by extensive clade-specific gene loss. Amino acid changes are distributed unequally across the gene tree, consistent with a neofunctionalization model of protein evolution. We consider our results in the context of early animal evolution, and the role of POU5 genes in maintaining stem cell pluripotency

Is globalization really to blame?

Money ; Capital movements ; Monetary policy ; Financial crises ; International finance ; Dollarization

Quantitative profiling of selective Sox/POU pairing on hundreds of sequences in parallel by Coop-seq

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. Cooperative binding of transcription factors is known to be important in the regulation of gene expression programs conferring cellular identities. However, current methods to measure cooperativity parameters have been laborious and therefore limited to studying only a few sequence variants at a time. We developed Coop-seq (cooperativity by sequencing) that is capable of efficiently and accurately determining the cooperativity parameters for hundreds of different DNA sequences in a single experiment. We apply Coop-seq to 12 dimer pairs from the Sox and POU families of transcription factors using 324 unique sequences with changed half-site orientation, altered spacing and discrete randomization within the binding elements. The study reveals specific dimerization profiles of different Sox factors with Oct4. By contrast, Oct4 and the three neural class III POU factors Brn2, Brn4 and Oct6 assemble with Sox2 in a surprisingly indistinguishable manner. Two novel half-site configurations can support functional Sox/Oct dimerization in addition to known composite motifs. Moreover, Coop-seq uncovers a nucleotide switch within the POU half-site when spacing is altered, which is mirrored in genomic loci bound by Sox2/Oct4 complexes.Link_to_subscribed_fulltex

Tracking Report 2011 Pou Chen, Indonesia 070033435J

This document is part of a digital collection provided by the Martin P. Catherwood Library, ILR School, Cornell University, pertaining to the effects of globalization on the workplace worldwide. Special emphasis is placed on labor rights, working conditions, labor market changes, and union organizing.FLA_2011_Pou_Chen_TR_Indonesia_070033435J.pdf: 9 downloads, before Oct. 1, 2020

Les caractéristiques de pou : un modal en position de complémenteur

Dans cet article, nous étudions les caractéristiques de pou en position de complémenteur. Nous identifions plus particulièrement le phénomène de référence libre ou disjointe en créole haïtien. Nous expliquons ce phénomène par les principes du Gouvernement et par la théorie du Liage. Par ailleurs, certains faits observés permettent de conclure que pou est un modal qui se déplace dans COMP par un mouvement de INFL à COMP.In this article we study the properties of pou as it occurs in complementizer position. In particular, we discuss the facts of reference in subordinate clauses. We explain these facts in terms of government and binding theory. We conclude that pou is actually a modal which moves to the position of the complementizer

An Analysis of the Microeconomic Determinants of Travel Frequency

A critical factor in predicting the demand for tourism within a certain period of time is the number of trips individuals take. New tourists’ behaviour shows a tendency toward more frequent travel. Nevertheless, the frequency of travel has received little attention in empirical literature. This paper uses household data to examine the determinants of the number of quarters with positive tourist expenditure within a year. The results highlight the relevance in travel frequency analyses of distinguishing between the participation decision and the frequency decision conditional on participation. Many socio-demographic variables only show explanatory power for the participation decision. The two most relevant factors by far in explaining each decision are the previous year tourism demand decisions (suggesting evidence of habit persistence in tourism decisions) and disposable income, although with an income elasticity below the unit.Tourism demand, frequency of travel, habit persistence, household data.

Interpreting & practicing kaupapa Māori research in a community setting: The in’s and out’s

Pou Tuia Rangahau is a unique community based research unit based within a kaupapa Māori organisation. Kaupapa Māori methodologies are utilised, with the importance of these methodologies being that Māori are defining the process, doing the research for and about Māori, with the eventual outcome being meaningful to Māori. This paper is placed within this wider context of Kaupapa Māori and how it applies to the practice of research in particular. We discuss how we interpret and practice Kaupapa Māori Research (KMR) within an urban community based organisation by highlighting a particular piece of research that was undertaken by Pou Tuia Rangahau, the Research Unit of Te Runanga O Kirikiriroa Trust Inc

Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter

Although the promoters of both the Bax and p21 genes are activated by p53, they differ in the effect on this activation of the POU family transcription factor Brn-3a. Thus, Brn-3a inhibits activation of the Bax promoter by p53 but enhances the ability of p53 to activate the p21 promoter. We demonstrate that repression of p53-mediated activation of the Bax promoter involves a complex upstream sequence in which two Brn-3a response elements flank the p53 response element. In contrast, a minimal p21 promoter is activated by Brn-3a and such activation cannot be abolished without abolishing basal promoter activity. Moreover, synergistic activation by Brn-3a and p53 continues to be observed when the p53-binding sites in the p21 promoter are substituted by the Bax p53 site or by the region of the Bax promoter essential for Brn-3a-mediated repression, indicating that the p21 core promoter plays a central role in this response. The significance of these effects is discussed in terms of the different responses of the Bax and p21 promoters and the overlapping but distinct roles of Brn-3a and p53 in neuronal growth arrest and apoptosis