Biotransformation of lanthanum by Aspergillus niger

Lanthanum is an important rare earth element and has many applications in modern electronics and catalyst manufacturing. However, there exist several obstacles in the recovery and cycling of this element due to a low average grade in exploitable deposits and low recovery rates by energy-intensive extraction procedures. In this work, a novel method to transform and recover La has been proposed using the geoactive properties of Aspergillus niger. La-containing crystals were formed and collected after A. niger was grown on Czapek-Dox agar medium amended with LaCl 3. Energy-dispersive X-ray analysis (EDXA) showed the crystals contained C, O, and La; scanning electron microscopy revealed that the crystals were of a tabular structure with terraced surfaces. X-ray diffraction identified the mineral phase of the sample as La 2(C 2O 4) 3·10H 2O. Thermogravimetric analysis transformed the oxalate crystals into La 2O 3 with the kinetics of thermal decomposition corresponding well with theoretical calculations. Geochemical modelling further confirmed that the crystals were lanthanum decahydrate and identified optimal conditions for their precipitation. To quantify crystal production, biomass-free fungal culture supernatants were used to precipitate La. The results showed that the precipitated lanthanum decahydrate achieved optimal yields when the concentration of La was above 15 mM and that 100% La was removed from the system at 5 mM La. Our findings provide a new aspect in the biotransformation and biorecovery of rare earth elements from solution using biomass-free fungal culture systems. </p

Human CLPP reverts the longevity phenotype of a fungal ClpP deletion strain

Mitochondrial maintenance crucially depends on the quality control of proteins by various chaperones, proteases and repair enzymes. While most of the involved components have been studied in some detail, little is known on the biological role of the CLPXP protease complex located in the mitochondrial matrix. Here we show that deletion of PaClpP, encoding the CLP protease proteolytic subunit CLPP, leads to an unexpected healthy phenotype and increased lifespan of the fungal ageing model organism Podospora anserina. This phenotype can be reverted by expression of human ClpP in the fungal deletion background, demonstrating functional conservation of human and fungal CLPP. Our results show that the biological role of eukaryotic CLP proteases can be studied in an experimentally accessible model organism

Oxygen adsorption on the Ru (10 bar 1 0) surface: Anomalous coverage dependence

Oxygen adsorption onto Ru (10 bar 1 0) results in the formation of two ordered overlayers, i.e. a c(2 times 4)-2O and a (2 times 1)pg-2O phase, which were analyzed by low-energy electron diffraction (LEED) and density functional theory (DFT) calculation. In addition, the vibrational properties of these overlayers were studied by high-resolution electron loss spectroscopy. In both phases, oxygen occupies the threefold coordinated hcp site along the densely packed rows on an otherwise unreconstructed surface, i.e. the O atoms are attached to two atoms in the first Ru layer Ru(1) and to one Ru atom in the second layer Ru(2), forming zigzag chains along the troughs. While in the low-coverage c(2 times 4)-O phase, the bond lengths of O to Ru(1) and Ru(2) are 2.08 A and 2.03 A, respectively, corresponding bond lengths in the high-coverage (2 times 1)-2O phase are 2.01 A and 2.04 A (LEED). Although the adsorption energy decreases by 220 meV with O coverage (DFT calculations), we observe experimentally a shortening of the Ru(1)-O bond length with O coverage. This effect could not be reconciled with the present DFT-GGA calculations. The nu(Ru-O) stretch mode is found at 67 meV [c(2 times 4)-2O] and 64 meV [(2 times 1)pg-2O].Comment: 10 pages, figures are available as hardcopies on request by mailing [email protected], submitted to Phys. Rev. B (8. Aug. 97), other related publications can be found at http://www.rz-berlin.mpg.de/th/paper.htm

Chromium (III) complexes and their relationship to the glucose tolerance factor : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Chemistry at Massey University

Two forms of the dinicotinate complex Cr(nic)2(H2O)3OH were formed which were yellow and blue, respectively. For the yellow form the nicotinic acid ligands were coordinated via the pyridine ring nitrogen atom but this complex was biologically inactive, while for the blue form nicotinic acid was coordinated via the carboxylate group and this compound was biologically active. Only Cr(III) formed a stable carboxylate coordinated dinicotinate complex. No stable complexes were formed with Fe(III) and Mn(III) due to significant olation, even at acidic pH's, and the complexes of nicotinic acid with Cr(II), Mn(II), Co(II) and Ni(II) were all pyridine nitrogen atom coordinated and biologically inactive. Several chromium (III) complexes with amino acids possessed biological activity also, and these included the α -carboxylate coordinated species Cr(gly)n(H2O)3+6-nand Cr(glu)n(H2O)3+6-n, the bidentate coordinated Cr(gln)2(H2O)2+ complex, and the NH4OH- eluted complexes obtained when Cr(gly)2(H2O)2+ and Cr(glu)2(H2O) 2+, but not Cr(cys) 2(H2O) 2+, were eluted from a DOWEX 50W-X12 cation-exchange column (loss of the α-amino coordination was postulated to have occurred). The biologically active mixed ligand complex postulated as Cr2(nic) 4(gly) 2(OH) 2 was prepared and found to be stable at neutral pH as a result of coordination of the glycine ligands. The activity of the chromium (III) complexes in the yeast fermentation assay suggested that similar effects would be found in mammalian systems. The yeast assay system was found to be a simple, quick and reproducible method of determining biological activity. All of the active chromium (III) complexes prepared were found to be similar, in structure, to the diguanide compound 1,4-diguanidinobutane which is known to lower blood sugar levels in mammals. This similarity in structure suggested a similar function might be possessed by the complexes reported in this thesi

Carbon Recombination Lines toward the Riegel-Crutcher Cloud and other Cold HI Regions in the inner Galaxy

We report here, for the first time, the association of low frequency CRRL with \HI\ self-absorbing clouds in the inner Galaxy and that the CRRLs from the innermost $\sim 10^{\circ}$ of the Galaxy arise in the Riegel-Crutcher (R-C) cloud. The R-C cloud is amongst the most well known of \HI\ self-absorbing (HISA) regions located at a distance of about 125 pc in the Galactic centre direction. Taking the R-C cloud as an example, we demonstrate that the physical properties of the HISA can be constrained by combining multi-frequency CRRL and \HI\ observations. The derived physical properties of the HISA cloud are used to determine the cooling and heating rates. The dominant cooling process is emission of the \CII\ 158 \mum line whereas dominant heating process in the cloud interior is photoelectric emission. Constraints on the FUV flux (G0 $\sim$ 4 to 7) falling on the R-C cloud are obtained by assuming thermal balance between the dominant heating and cooling processes. The H$_2$ formation rate per unit volume in the cloud interior is $\sim$ 10$^{-10}$ -- 10$^{-12}$ s$^{-1}$ \cmthree, which far exceeds the H$_2$ dissociation rate per unit volume. We conclude that the self-absorbing cold \HI\ gas in the R-C cloud may be in the process of converting to the molecular form. The cold \HI\ gas observed as HISA features are ubiquitous in the inner Galaxy and form an important part of the ISM. Our analysis shows that combining CRRL and \HI\ data can give important insight into the nature of these cold gas. We also estimate the integration times required to image the CRRL forming region with the upcoming SKA pathfinders. Imaging with the MWA telescope is feasible with reasonable observing times.Comment: 11 pages, 4 figures, 5 tables, accepted by MNRA

PPPC 4 DM secondary: A Poor Particle Physicist Cookbook for secondary radiation from Dark Matter

We enlarge the set of recipes and ingredients at disposal of any poor particle physicist eager to cook up signatures from weak-scale Dark Matter models by computing two secondary emissions due to DM particles annihilating or decaying in the galactic halo, namely the radio signals from synchrotron emission and the gamma rays from bremsstrahlung. We consider several magnetic field configurations and propagation scenarios for electrons and positrons. We also provide an improved energy loss function for electrons and positrons in the Galaxy, including synchrotron losses in the different configurations, bremsstrahlung losses, ionization losses and Inverse Compton losses with an updated InterStellar Radiation Field.Comment: 25 pages, many figures. v2: a small clarification on the use of custom galactic magnetic fields added, matches version published on JCAP. All results are available at http://www.marcocirelli.net/PPPC4DMID.htm

Some aspects of electrical conduction in granular systems of various dimensions

We report on measurements of the electrical conductivity in both a 2D triangular lattice of metallic beads and in a chain of beads. The voltage/current characteristics are qualitatively similar in both experiments. At low applied current, the voltage is found to increase logarithmically in a good agreement with a model of widely distributed resistances in series. At high enough current, the voltage saturates due to the local welding of microcontacts between beads. The frequency dependence of the saturation voltage gives an estimate of the size of these welded microcontacts. The DC value of the saturation voltage (~ 0.4 V per contact) gives an indirect measure of the number of welded contact carrying the current within the 2D lattice. Also, a new measurement technique provides a map of the current paths within the 2D lattice of beads. For an isotropic compression of the 2D granular medium, the current paths are localized in few discrete linear paths. This quasi-onedimensional nature of the electrical conductivity thus explains the similarity between the characteristics in the 1D and 2D systems.Comment: To be published in The European Physical Journal