Novel parvoviruses from wild and domestic animals in Brazil provide new insights into parvovirus distribution and diversity

Parvoviruses (family Parvoviridae) are small, single-stranded DNA viruses. Many parvoviral pathogens of medical, veterinary and ecological importance have been identified. In this study, we used high-throughput sequencing (HTS) to investigate the diversity of parvoviruses infecting wild and domestic animals in Brazil. We identified 21 parvovirus sequences (including twelve nearly complete genomes and nine partial genomes) in samples derived from rodents, bats, opossums, birds and cattle in Pernambuco, São Paulo, Paraná and Rio Grande do Sul states. These sequences were investigated using phylogenetic and distance-based approaches and were thereby classified into eight parvovirus species (six of which have not been described previously), representing six distinct genera in the subfamily Parvovirinae. Our findings extend the known biogeographic range of previously characterized parvovirus species and the known host range of three parvovirus genera (Dependovirus, Aveparvovirus and Tetraparvovirus). Moreover, our investigation provides a window into the ecological dynamics of parvovirus infections in vertebrates, revealing that many parvovirus genera contain well-defined sub-lineages that circulate widely throughout the world within particular taxonomic groups of hosts

On the development of the chondrocranium and the histological anatomy of the head in perinatal stages of marsupial mammals

An overview of the literature on the chondrocranium of marsupial mammals reveals a relative conservatism in shape and structures. We document the histological cranial anatomy of individuals representing Monodelphis domestica, Dromiciops gliroides, Perameles sp. and Macropus eugenii. The marsupial chondrocranium is generally characterized by the great breadth of the lamina basalis, absence of pila metoptica and large otic capsules. Its most anterior portion (cupula nasi anterior) is robust, and anterior to it there are well-developed tactile sensory structures, functionally important in the neonate. Investigations of ossification centers at and around the nasal septum are needed to trace the presence of certain bones (e.g., mesethmoid, parasphenoid) across marsupial taxa. In many adult marsupials, the tympanic floor is formed by at least three bones: alisphenoid (alisphenoid tympanic process), ectotympanic and petrosal (rostral and caudal tympanic processes); the squamosal also contributes in some diprotodontians. The presence of an entotympanic in marsupials has not been convincingly demonstrated. The tubal element surrounding the auditory tube in most marsupials is fibrous connective tissue rather than cartilage; the latter is the case in most placentals recorded to date. However, we detected fibrocartilage in a late juvenile of Dromiciops, and a similar tissue has been reported for Tarsipes. Contradictory reports on the presence of the tegmen tympani can be found in the literature. We describe a small tegmen tympani in Macropus. Several heterochronic shifts in the timing of development of the chondocranium and associated structures (e.g., nerves, muscles) and in the ossification sequence have been interpreted as largely being influenced by functional requirements related to the altriciality of the newborn marsupial during early postnatal life. Comparative studies of chondocranial development of mammals can benefit from a solid phylogenetic framework, research on non-classical model organisms, and integration with imaging and sectional data derived from computer-tomography.Fil: Sánchez Villagra, Marcelo R.. Universitat Zurich; SuizaFil: Forasiepi, Analia Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentin

Spatial epidemiological patterns suggest mechanisms of land-sea transmission for Sarcocystis neurona in a coastal marine mammal.

Sarcocystis neurona was recognised as an important cause of mortality in southern sea otters (Enhydra lutris nereis) after an outbreak in April 2004 and has since been detected in many marine mammal species in the Northeast Pacific Ocean. Risk of S. neurona exposure in sea otters is associated with consumption of clams and soft-sediment prey and is temporally associated with runoff events. We examined the spatial distribution of S. neurona exposure risk based on serum antibody testing and assessed risk factors for exposure in animals from California, Washington, British Columbia and Alaska. Significant spatial clustering of seropositive animals was observed in California and Washington, compared with British Columbia and Alaska. Adult males were at greatest risk for exposure to S. neurona, and there were strong associations with terrestrial features (wetlands, cropland, high human housing-unit density). In California, habitats containing soft sediment exhibited greater risk than hard substrate or kelp beds. Consuming a diet rich in clams was also associated with increased exposure risk. These findings suggest a transmission pathway analogous to that described for Toxoplasma gondii, with infectious stages traveling in freshwater runoff and being concentrated in particular locations by marine habitat features, ocean physical processes, and invertebrate bioconcentration

The Overlap Package

Camera traps - cameras linked to detectors so that they fire when an animal is present - are a major source of information on the abundance and habitat preferences of rare or shy forest animals. Modern cameras record the time of the photo, and the use of this to investigate diel activity patterns was immediately recognised (Gri?ffiths and van Schaik, 1993). Initially this resulted in broad classfication of taxa as diurnal, nocturnal, crepuscular, or cathemeral (van Schaik and Gri?ths, 1996). More recently, researchers have compared activity patterns among species to see how overlapping patterns may relate to competition or predation (Linkie and Ridout, 2011; Carver et al., 2011; Ramesh et al., 2012; Carter et al., 2012; Kamler et al., 2012; Ross et al., 2013). Ridout and Linkie (2009) presented methods to fit kernel density functions to times of observations of animals and to estimate the coe?cient of overlapping, a quantitative measure ranging from 0 (no overlap) to 1 (identical activity patterns). The code they used forms the basis of the overlap package. Although motivated by the analysis of camera trap data, overlap could be applied to data from other sources such as data loggers, provided data collection is carried out around the clock. Nor is it limited to diel cycles: tidal cycles or seasonal cycles, such as plant flowering or fruiting or animal breeding seasons could also be investigated

Some Aspects of the Ecology of the Virginia Opossum (Didelphis virginiana virginiana Kerr 1792) in an Urban Environment

Eighty-three Didelphis virginiana from the Portland metropolitan area were collected and studied from December 1974 through May 1976. Males averaged 3394 g, females 2318 g. An annual sex ratio of 68.7:31.3 was recorded. Data indicate the possibility of a year-round breeding season and suggest the occurrence of three peaks of reproductive activity: January-February, April-May and October-November. The six primary foods consumed annually are: leaf litter, mammals, gastropods, earthworms, grasses and pet food. Seasonal composition and diversity in the diet is marked. Five new parasite-host records were established. These include mites: Androlaelaps fahrenholsi, Haemogamasus nidi, Eulaelape stabularis tick, Ixodes angustus, and a nematode, Toxocara canis. Both minimum and maximum temperature, and perhaps precipitation to a lesser degree, affect the activity of the species. Nest building by an immature opossum is described

Relationship between moonlight and nightly activity patterns of the ocelot (Leopardus pardalis) and some of its prey species in Formosa, Northern Argentina

The moon can profoundly influence the activity patterns of animals. If predators are more successful under bright moonlight, prey species are likely to respond by shifting their own activity patterns (predator-avoidance hypothesis). However, the assumption that prey will necessarily avoid full-moon nights does not take into account that moonlight also allows prey to more easily detect predators, and to forage more efficiently. Thus, nightly activity patterns could depend on night vision capabilities (visual-acuity hypothesis). To consider the possible influences of moonlight and to distinguish between these hypotheses, we used camera-trapping records of a predator, the ocelot (Leopardus pardalis), and several of its night-active prey to compare activity patterns under different moonlight conditions. The ocelots' activity patterns were not strongly related to moonlight, but showed a slight tendency for higher activity during brighter nights. Tapeti rabbits (Sylvilagus brasiliensis) and brocket deer (Mazama americana) showed a clear preference for brighter nights. White-eared opossums (Didelphis albiventris) also showed a trend to be less active in new moon light. In contrast, smaller grey four-eyed opossums (Philander opossum) and the poor eye-sight nine-banded armadillo (Dasypus novemcinctus) showed similar activity patterns across all moon phases. Since activity patterns of most prey species were not shifted away from the activity of the ocelot, the differences between species are probably linked to their night vision capabilities, and emphasise the need for more information on the visual system of these taxa. Their activity patterns seem to be less strongly linked to avoidance of predation than previously thought, suggesting that foraging and predator detection benefits may play a more important role than usually acknowledged.The cameras were partly funded by a National Geographic Society/Waitt grant to MH (grant number NGS 1072-78) and partly by quality-related funding through the Research Excellence Framework to the Biological Sciences Research Group of the University of Derby. The long-term camera-trap monitoring was in part made possible through grants to EFD and the Owl Monkey Project from the L.S.B. Leakey Foundation, National Geographic Society and the National Science Foundation of the USA (NSF-BCS-0621020, 0837921 (REU), 0924352 (REU), 1026991 (REU), 1219368 (RAPID) and 1232349 (2012)

Establishing macroecological trait datasets: digitalization, extrapolation, and validation of diet preferences in terrestrial mammals worldwide

Ecological trait data are essential for understanding the broad-scale distribution of biodiversity and its response to global change. For animals, diet represents a fundamental aspect of species’ evolutionary adaptations, ecological and functional roles, and trophic interactions. However, the importance of diet for macroevolutionary and macroecological dynamics remains little explored, partly because of the lack of comprehensive trait datasets. We compiled and evaluated a comprehensive global dataset of diet preferences of mammals (“MammalDIET”). Diet information was digitized from two global and cladewide data sources and errors of data entry by multiple data recorders were assessed. We then developed a hierarchical extrapolation procedure to fill-in diet information for species with missing information. Missing data were extrapolated with information from other taxonomic levels (genus, other species within the same genus, or family) and this extrapolation was subsequently validated both internally (with a jack-knife approach applied to the compiled species-level diet data) and externally (using independent species-level diet information from a comprehensive continentwide data source). Finally, we grouped mammal species into trophic levels and dietary guilds, and their species richness as well as their proportion of total richness were mapped at a global scale for those diet categories with good validation results. The success rate of correctly digitizing data was 94%, indicating that the consistency in data entry among multiple recorders was high. Data sources provided species-level diet information for a total of 2033 species (38% of all 5364 terrestrial mammal species, based on the IUCN taxonomy). For the remaining 3331 species, diet information was mostly extrapolated from genus-level diet information (48% of all terrestrial mammal species), and only rarely from other species within the same genus (6%) or from family level (8%). Internal and external validation showed that: (1) extrapolations were most reliable for primary food items; (2) several diet categories (“Animal”, “Mammal”, “Invertebrate”, “Plant”, “Seed”, “Fruit”, and “Leaf”) had high proportions of correctly predicted diet ranks; and (3) the potential of correctly extrapolating specific diet categories varied both within and among clades. Global maps of species richness and proportion showed congruence among trophic levels, but also substantial discrepancies between dietary guilds. MammalDIET provides a comprehensive, unique and freely available dataset on diet preferences for all terrestrial mammals worldwide. It enables broad-scale analyses for specific trophic levels and dietary guilds, and a first assessment of trait conservatism in mammalian diet preferences at a global scale. The digitalization, extrapolation and validation procedures could be transferable to other trait data and taxa

Catalogue of the ectoparasitic insects of the bats of Argentina

Taxonomy and distribution of the ectoparasitic insects of bats from Argentina, are reviewed. Seventeen species of Diptera (six of Nycteribiidae and eleven of Streblidae), six species of Siphonaptera (four ofIschnopsyllidae, one of Pulicidae, and one ofStephanocircidae), and seven species of Hemiptera (Polyctenidae) are known presently for Argentina. The information was obtained by reviewing the literature and collecting in the field between 1989 and 1998. The specimens collected in the field were compared with the type material deposited at the Field Museum of Natural History (CHNM).En este primer catalogo de insectos ectoparasitos de murcielagos de la Argentina, se ofrece informacion sobre taxonomia y distribucion. Se conocen actualmente en el pais 17 especies de Diptera (seis de Nycteribiidae yonce de Streblidae), seis de Siphonaptera (cuatro de Ischnopsyllidae, una de Pulicidae y una de Stephanocircidae) y siete especies de Hemiptera (Polyctenidae). Se consulto numerosa literatura sobre los distintos grupos y se reviso abundante material obtenido en viajes de campana realizados desde 1989 a 1998, a numerosas localidades de la Argentina. Se realizaron comparaciones con material tipo del Field Museum of Natural History (CHNM)

Common distribution patterns of marsupials related to physiographical diversity in Venezuela

The aim of this study is to identify significant biotic regions (groups of areas with similar biotas) and biotic elements (groups of taxa with similar distributions) for the marsupial fauna in a part of northern South America using physiographical areas as Operational Geographical Units (OGUs). We considered Venezuela a good model to elucidate this issue because of its high diversity in landscapes and the relatively vast amount of information available on the geographical distribution of marsupial species. Based on the presence-absence of 33 species in 15 physiographical sub-regions (OGUs) we identified Operational Biogeographical Units (OBUs) and chorotypes using a quantitative analysis that tested statistical significance of the resulting groups. Altitudinal and/or climatic trends in the OBUs and chorotypes were studied using a redundancy analysis. The classification method revealed four OBUs. Strong biotic boundaries separated: i) the xerophytic zone of the Continental coast (OBU I); ii) the sub-regions north of the Orinoco River (OBU III and IV); and those south to the river (OBU II). Eleven chorotypes were identified, four of which included a single species with a restricted geographic distribution. As for the other chorotypes, three main common distribution patterns have been inferred: i) species from the Llanos and/or distributed south of the Orinoco River; ii) species exclusively from the Andes; and iii) species that either occur exclusively north of the Orinoco River or that show a wide distribution throughout Venezuela. Mean altitude, evapotranspiration and precipitation of the driest month, and temperature range allowed us to characterize environmentally most of the OBUs and chorotypes obtained