Physical and electrophysiological motor unit characteristics are revealed with simultaneous high-density electromyography and ultrafast ultrasound imaging

Electromyography and ultrasonography provide complementary information about electrophysiological and physical (i.e. anatomical and mechanical) muscle properties. In this study, we propose a method to assess the electrical and physical properties of single motor units (MUs) by combining High-Density surface Electromyography (HDsEMG) and ultrafast ultrasonography (US). Individual MU firings extracted from HDsEMG were used to identify the corresponding region of muscle tissue displacement in US videos. The time evolution of the tissue velocity in the identified region was regarded as the MU tissue displacement velocity. The method was tested in simulated conditions and applied to experimental signals to study the local association between the amplitude distribution of single MU action potentials and the identified displacement area. We were able to identify the location of simulated MUs in the muscle cross-section within a 2 mm error and to reconstruct the simulated MU displacement velocity (cc > 0.85). Multiple regression analysis of 180 experimental MUs detected during isometric contractions of the biceps brachii revealed a significant association between the identified location of MU displacement areas and the centroid of the EMG amplitude distribution. The proposed approach has the potential to enable non-invasive assessment of the electrical, anatomical, and mechanical properties of single MUs in voluntary contractions

A denoising algorithm for surface EMG decomposition

The goal of the present thesis was to investigate a novel motor unit potential train (MUPT) editing routine, based on decreasing the variability in shape (variance ratio, VR) of the MUP ensemble. Decomposed sEMG data from 20 participants at 60% MVC of wrist flexion was used. There were two levels of denoising (relaxed and strict) criteria for removing discharge times associated with waveforms that did not decrease the VR and increase its signal-to-noise ratio (SNR) of the MUP ensemble. The peak-to-peak amplitude and the duration between the positive and negative peaks for the MUP template were dependent on the level of denoising (p’s 0.05). The same was true between denoising criteria (p>0.05). Editing the MUPT based on MUP shape resulted in significant differences in measures extracted from the MUP template, with trivial difference between the standard error of estimate for mean IDIs between the complete and denoised MUPTs

Applications of EMG in Clinical and Sports Medicine

This second of two volumes on EMG (Electromyography) covers a wide range of clinical applications, as a complement to the methods discussed in volume 1. Topics range from gait and vibration analysis, through posture and falls prevention, to biofeedback in the treatment of neurologic swallowing impairment. The volume includes sections on back care, sports and performance medicine, gynecology/urology and orofacial function. Authors describe the procedures for their experimental studies with detailed and clear illustrations and references to the literature. The limitations of SEMG measures and methods for careful analysis are discussed. This broad compilation of articles discussing the use of EMG in both clinical and research applications demonstrates the utility of the method as a tool in a wide variety of disciplines and clinical fields

Analysis of forearm muscles activity by means of new protocols of multichannel EMG signal recording and processing

Los movimientos voluntarios del cuerpo son controlados por el sistema nervioso central y periférico a través de la contracción de los músculos esqueléticos. La contracción se inicia al liberarse un neurotransmisor sobre la unión neuromuscular, iniciando la propagación de un biopotencial sobre la membrana de las fibras musculares que se desplaza hacia los tendones: el Potencial de Acción de la Unidad Motora (MUAP). La señal electromiográfica de superficie registra la activación continua de dichos potenciales sobre la superficie de la piel y constituye una valiosa herramienta para la investigación, diagnóstico y seguimiento clínico de trastornos musculares, así como para la identificación de la intención movimiento tanto en términos de dirección como de potencia. En el estudio de las enfermedades del sistema neuromuscular es necesario analizar el nivel de actividad, la capacidad de producción de fuerza, la activación muscular conjunta y la predisposición a la fatiga muscular, todos ellos asociados con factores fisiológicos que determinan la resultante contracción mioeléctrica. Además, el uso de matrices de electrodos facilita la investigación de las propiedades periféricas de las unidades motoras activas, las características anatómicas del músculo y los cambios espaciales en su activación, ocasionados por el tipo de tarea motora o la potencia de la misma. El objetivo principal de esta tesis es el diseño e implementación de protocolos experimentales y algoritmos de procesado para extraer información fiable de señales sEMG multicanal en 1 y 2 dimensiones del espacio. Dicha información ha sido interpretada y relacionada con dos patologías específicas de la extremidad superior: Epicondilitis Lateral y Lesión de Esfuerzo Repetitivo. También fue utilizada para identificar la dirección de movimiento y la fuerza asociada a la contracción muscular, cuyos patrones podrían ser de utilidad en aplicaciones donde la señal electromiográfica se utilice para controlar interfaces hombre-máquina como es el caso de terapia física basada en robots, entornos virtuales de rehabilitación o realimentación de la actividad muscular. En resumen, las aportaciones más relevantes de esta tesis son: * La definición de protocolos experimentales orientados al registro de señales sEMG en una región óptima del músculo. * Definición de índices asociados a la co-activación de diferentes músculos * Identificación de señales artefactuadas en registros multicanal * Selección de los canales mas relevantes para el análisis Extracción de un conjunto de características que permita una alta exactitud en la identificación de tareas motoras Los protocolos experimentales y los índices propuestos permitieron establecer que diversos desequilibrios entre músculos extrínsecos del antebrazo podrían desempeñar un papel clave en la fisiopatología de la epicondilitis lateral. Los resultados fueron consistentes en diferentes ejercicios y pueden definir un marco de evaluación para el seguimiento y evaluación de pacientes en programas de rehabilitación motora. Por otra parte, se encontró que las características asociadas con la distribución espacial de los MUAPs mejoran la exactitud en la identificación de la intención de movimiento. Lo que es más, las características extraídas de registros sEMG de alta densidad son más robustas que las extraídas de señales bipolares simples, no sólo por la redundancia de contacto implicada en HD-EMG, sino también porque permite monitorizar las regiones del músculo donde la amplitud de la señal es máxima y que varían con el tipo de ejercicio, permitiendo así una mejor estimación de la activación muscular mediante el análisis de los canales mas relevantes.Voluntary movements are achieved by the contraction of skeletal muscles controlled by the Central and Peripheral Nervous system. The contraction is initiated by the release of a neurotransmitter that promotes a reaction in the walls of the muscular fiber, producing a biopotential known as Motor Unit Action Potential (MUAP) that travels from the neuromuscular junction to the tendons. The surface electromyographic signal records the continuous activation of such potentials over the surface of the skin and constitutes a valuable tool for the diagnosis, monitoring and clinical research of muscular disorders as well as to infer motion intention not only regarding the direction of the movement but also its power. In the study of diseases of the neuromuscular system it is necessary to analyze the level of activity, the capacity of production of strength, the load-sharing between muscles and the probably predisposition to muscular fatigue, all of them associated with physiological factors determining the resultant muscular contraction. Moreover, the use of electrode arrays facilitate the investigation of the peripheral properties of the active Motor Units, the anatomical characteristics of the muscle and the spatial changes induced in their activation of as product of type of movement or power of the contraction.The main objective of this thesis was the design and implementation of experimental protocols, and algorithms to extract information from multichannel sEMG signals in 1 and 2 dimensions of the space. Such information was interpreted and related to pathological events associated to two upper-limb conditions: Lateral Epicondylitis and Repetitive Strain Injury. It was also used to identify the direction of movement and contraction strength which could be useful in applications concerning the use of biofeedback from EMG like in robotic- aided therapies and computer-based rehabilitation training.In summary, the most relevant contributions are:§The definition of experimental protocols intended to find optimal regions for the recording of sEMG signals. §The definition of indices associated to the co- activation of different muscles. §The detection of low-quality signals in multichannel sEMG recordings.§ The selection of the most relevant EMG channels for the analysis§The extraction of a set of features that led to high classification accuracy in the identification of tasks.The experimental protocols and the proposed indices allowed establishing that imbalances between extrinsic muscles of the forearm could play a key role in the pathophysiology of lateral epicondylalgia. Results were consistent in different types of motor task and may define an assessment framework for the monitoring and evaluation of patients during rehabilitation programs.On the other hand, it was found that features associated with the spatial distribution of the MUAPs improve the accuracy of the identification of motion intention. What is more, features extracted from high density EMG recordings are more robust not only because it implies contact redundancy but also because it allows the tracking of (task changing) skin surface areas where EMG amplitude is maximal and a better estimation of muscle activity by the proper selection of the most significant channels

Age-Sensitive Features for Detection of Muscle Fatigue using the High-Density Electromyogram

The processes behind fatigue development within the muscles have been a topic of interest for exercise scientists for decades. This is because fatigue is one of the primary reasons for a decrease in performance and increase in likelihood of injury during exercise[1]. Typically, muscle fatigue is detected through modifications of the amplitude and spectral characteristics of a surface electromyogram (sEMG), or the variability of torque signals recorded throughout a sustained contraction. However, the behaviour of these parameters with the generation of fatigue depends on a variety of factors. One major factor is age, where the age-related loss of muscle fibers, and changes in neuromuscular system impact how muscles adapt to and develop fatigue. The purpose of this study was to examine age-sensitive High Density Surface Electromyogram (HD-sEMG) features and investigate the effect of spatial filter type on intramuscular coherence analysis in fatigue detection. Fatiguing submaximal isometric contractions of the bicep brachii was performed by eight young (24.40 ± 2.42 years) and five elderly (72.90 ± 2.21 years) males, while HD-sEMG recorded signals from the biceps brachii and a dynamometer recorded torque signals. The task was performed at 20% maximal voluntary contraction (MVC). From the HD-sEMG signals, the mean intramuscular coherence was calculated in the alpha (11-15Hz), beta (16-29Hz), and gamma (30-50Hz) frequency bands each of which stems from different neurological origins. Statistical differences were only found in the alpha (p=0.0006), and beta (p=0.0207) bands between the pre-and post-fatigue conditions of the young group. Furthermore, a correlation between mean coherence and torque variability during the final 25% of the contraction before task failure revealed that both the age groups had positive correlation in the alpha band. Different correlations were found in the beta and gamma bands, with positive correlations being observed in the elderly group and negative correlations in the young group. These results suggest that age-related changes in the corticospinal pathway exist causing the elderly to be less fatigable when compared to the young population. This proposes that the introduced intramuscular coherence analysis can be used to obtain fatigue related features from HD-sEMG signals that are age-sensitive

Evaluation of performance fatigability through surface EMG in health and muscle disease: state of the art

In literature, it is commonly reported that the progress of performance fatigability may be indirectly assessed through the changes in the features of the surface electromyogram (sEMG) signal. In particular, during isometric constant force contractions, changes in the sEMG signal are caused by several physiological factors, such as a decay in muscle fibers conduction velocity (CV), an increase of the degree of synchronization between the firing times of simultaneously active motor units (MUs), by the central nervous system, and a reduction of the recruitment threshold and a modulation of MUs firing rate. Amplitude and spectral parameters may be used to characterize the global contributions to performance fatigability, such as MU control properties and fiber membrane properties, or central and peripheral factors, respectively. In addition, being CV a physiological parameter, its estimation is of marked interest to the study of fatigue both in physiological and in presence of neuromuscular diseases

Surface Electromyographic (sEMG) Transduction of Hand Joint Angles for Human Interfacing Devices (HID)

This is an investigation of the use of surface electromyography (sEMG) as a tool to improve human interfacing devices (HID) information bandwidth through the transduction of the fingertip workspace. It combines the work of Merletti et al and Jarque-Bou et al to design an open-source framework for Fingertip Workspace based Human Interfacing Devices (HID). In this framework, the fingertip workspace is defined as the system of forearm and hand muscle force through a tensor which describes hand anthropometry. The thesis discusses the electrophysiology of muscle tissue along with the anatomy and physiology of the arm in pursuit of optimizing sensor location, muscle force measurements, and viable command gestures. Algorithms for correlating sEMG to hand joint angle are investigated using MATLAB for both static and moving gestures. Seven sEMG spots and Fingertip Joint Angles recorded by Jarque Bou et al are investigated for the application of sEMG to Human Interfacing Devices (HID). Such technology is termed Gesture Computer Interfacing (GCI) and has been shown feasible through devices such as CTRL Labs interface, and models such as those of Sartori, Merletti, and Zhao. Muscles under sEMG spots in this dataset and the actions related to them are discussed, along with what muscles and hand actions are not visible within this dataset. Viable gestures for detection algorithms are discussed based on the muscles discerned to be visible in the dataset through intensity, spectral moment, power spectra, and coherence. Detection and isolation of such viable actions is fundamental to designing an EMG driven musculoskeletal model of the hand needed to facilitate GCI. Enveloping, spectral moment, power spectrum, and coherence analysis are applied to a Sollerman Hand Function Test sEMG dataset of twenty-two subjects performing 26 activities of living to differentiate pinching and grasping tasks. Pinches and grasps were found to cause very different activation patterns in sEMG spot 3 relating to flexion of digits I - V. Spectral moment was found to be less correlated with differentiation and provided information about the degree of object manipulation performed and extent of fatigue during each task. Coherence was shown to increase between flexors and extensors with intensity of task but was found corrupted by crosstalk with increasing intensity of muscular activation. Some spectral results correlated between finger flexor and extensor power spectra showed anticipatory coherence between the muscle groups at the end of object manipulation. An sEMG amplification system capable of capturing HD-sEMG with a bandwidth of 300 and 500 Hz at a sampling frequency of 2 kHz was designed for future work. The system was designed in ordinance with current IEEE research on sensor-electrode characteristics. Furthermore, discussion of solutions to open issues in HD-sEMG is provided. This work did not implement the designed wristband but serves as a literature review and open-source design using commercially available technologies

Biomechanics and Electromyography Inassessing Female Stress Urinary Incontinence

Introduction: Stress urinary incontinence (SUI), the involuntary urinary leakage associated with increases in intra-abdominal pressure, has a prevalence of 25–50% in U.S. women and the number of those who will undergo surgery will increase by half in the next forty years. SUI negatively affects the patient’s quality of life and places a great burden to the society. The functional anatomy of the continence mechanism remains vaguely understood. Hence my dissertation aims at offering a complete description of the pelvic floor muscles (PFM), the key contributor to the continence, thorough biomechanical and neurophysiological approaches. Methods: The biomechanical approach involves the development of a subject-specific finite element (FE) model of the female pelvic floor region. Subsequent computer simulations are targeted at finding the most contributive muscle to the urethral support function and evaluating current treatment strategies using a mini-sling. The neurophysiological approach involves the implementation of a novel surface electromyography (EMG) probe to acquire bioelectrical information of PFMs and the assessment of their innervations in healthy subjects and patients. Results: An FE pelvic floor model was developed which incorporates 40+ anatomical structural in the pelvis, representing the most complete model in the field. Simulation results showed that the vaginal walls, puborectalis, and pubococcygeus are the most important structures and that mid-distal post-urethral implantation represents the optimal location. Innervation zones of PFMs have been successfully identified and described for multiple PFMs. An high-density surface EMG-based motor unit number estimation approach was developed, providing a novel tool to evaluate the condition of neurologically impaired PFM. Conclusions: The combined information greatly advances our understanding of the physiology of PFM and would lay a firm foundation to novel, non-invasive, patient-specific interventional strategies in the future.Biomedical Engineering, Department o

Deep Learning Methods for Hand Gesture Recognition via High-Density Surface Electromyogram (HD-sEMG) Signals

Hand Gesture Recognition (HGR) using surface Electromyogram (sEMG) signals can be considered as one of the most important technologies in making efficient Human Machine Interface (HMI) systems. In particular, sEMG-based hand gesture has been a topic of growing interest for development of assistive systems to improve the quality of life in individuals suffering from amputated limbs. Generally speaking, myoelectric prosthetic devices work by classifying existing patterns of the collected sEMG signals and synthesizing intended gestures. While conventional myoelectric control systems, e.g., on/off control or direct-proportional, have potential advantages, challenges such as limited Degree of Freedom (DoF) due to crosstalk have resulted in the emergence of data-driven solutions. More specifically, to improve efficiency, intuitiveness, and the control performance of hand prosthetic systems, several Artificial Intelligence (AI) algorithms ranging from conventional Machine Learning (ML) models to highly complicated Deep Neural Network (DNN) architectures have been designed for sEMG-based hand gesture recognition in myoelectric prosthetic devices. In this thesis, we, first, perform a literature review on hand gesture recognition methods and elaborate on the recently proposed Deep Learning/Machine Learning (DL/ML) models in the literature. Then, our utilized High-Density sEMG (HD-sEMG) dataset is introduced and the rationales behind our main focus on this particular type of sEMG dataset are explained. We, then, develop a Vision Transformer (ViT)-based model for gesture recognition with HD-sEMG signals and evaluate its performance under different conditions such as variable window sizes, number of electrode channels, and model's complexity. We compare its performance with that of two conventional ML and one DL algorithm that are typically adopted in this domain. Furthermore, we introduce another capability of our proposed framework for instantaneous training, which is its ability to classify hand gestures based on a single frame of HD-sEMG dataset. Following that, we introduce the idea of integrating the macroscopic and microscopic neural drive information obtained from HD-sEMG data into a hybrid ViT-based framework for gesture recognition, which outperforms a standalone ViT architecture in terms of classification accuracy. Here, microscopic neural drive information (also called Motor Unit Spike Trains) refers to the neural commands sent by the brain and spinal cord to individual muscle fibers and are extracted from HD-sEMG signals using Blind Source Separation (BSP) algorithms. Finally, we design an alternative and novel hand gesture recognition model based on the less-explored topic of Spiking Neural Networks (SNN), which performs spatio-temporal gesture recognition in an event-based fashion. As opposed to the classical DNN architectures, SNNs are of the capacity to imitate human brain's cognitive function by using biologically inspired models of neurons and synapses. Therefore, they are more biologically explainable and computationally efficient