Application of a rule-based knowledge system using CLIPS for the taxonomy of selected Opuntia species

A rule based knowledge system was developed in CLIPS (C Language Integrated Production System) for identifying Opuntia species in the family Cactaceae, which contains approx. 1500 different species. This botanist expert tool system is capable of identifying selected Opuntia plants from the family level down to the species level when given some basic characteristics of the plants. Many plants are becoming of increasing importance because of their nutrition and human health potential, especially in the treatment of diabetes mellitus. The expert tool system described can be extremely useful in an unequivocal identification of many useful Opuntia species

Gas exchanges and water-use efficiency of Nopalea cochenillifera intercropped under edaphic practices

The Nopalea forage cactus (Nopalea cochenillifera) is the main xerophilic species cultivated in Brazil, highlighted as a compatible biological element with the semiarid environment. However, studies on its physiological aspects are still scarce. In this context, an experiment was performed in the Pendência Experimental Station, belonging to the State Company of Agricultural Research of Paraíba (EMEPA-PB), municipality of Soledade, state of Paraíba, Brazil, aiming to evaluate the gas exchanges and the water-use efficiency of intercropped Nopalea Cochenillifera plants in the soil under different edaphic managements. The treatments were distributed in randomized blocks, with three replications in a 2 × 5 factorial scheme, corresponding to the soil without and with mulch and five types of cultivation of Nopalea forage cactus, in monoculture and intercropped with forage watermelon, millet, sorghum, and buffelgrass. The analyzed variables were: stomatal conductance, transpiration rate, net photosynthesis rate, and internal CO2 concentration, besides the instantaneous water-use efficiency, intrinsic water-use efficiency, and instantaneous carboxylation efficiency. The use of soil mulch satisfactorily increased the photosynthetic rate of the forage cactus; this type of practice can aid in the productive development of the crop in semiarid zones, whereas the intercropping with buffelgrass and forage watermelon stimulated photosynthesis and the water-use efficiency.The Nopalea forage cactus (Nopalea cochenillifera) is the main xerophilic species cultivated in Brazil, highlighted as a compatible biological element with the semiarid environment. However, studies on its physiological aspects are still scarce. In this context, an experiment was performed in the Pendência Experimental Station, belonging to the State Company of Agricultural Research of Paraíba (EMEPA-PB), municipality of Soledade, state of Paraíba, Brazil, aiming to evaluate the gas exchanges and the water-use efficiency of intercropped Nopalea Cochenillifera plants in the soil under different edaphic managements. The treatments were distributed in randomized blocks, with three replications in a 2 × 5 factorial scheme, corresponding to the soil without and with mulch and five types of cultivation of Nopalea forage cactus, in monoculture and intercropped with forage watermelon, millet, sorghum, and buffelgrass. The analyzed variables were: stomatal conductance, transpiration rate, net photosynthesis rate, and internal CO2 concentration, besides the instantaneous water-use efficiency, intrinsic water-use efficiency, and instantaneous carboxylation efficiency. The use of soil mulch satisfactorily increased the photosynthetic rate of the forage cactus; this type of practice can aid in the productive development of the crop in semiarid zones, whereas the intercropping with buffelgrass and forage watermelon stimulated photosynthesis and the water-use efficiency

Occurrence of pests and diseases in cactus pear genotypes / Ocorrência de pragas e doenças em genótipos de palma forrageira

The objective was to evaluate the occurrence of pests, diseases and mortality rate in nine genotypes of cactus pear (Nopalea cochenillifera) destined for forage production. The genotypes were implanted in a complete randomized block design, with nine treatments and three replications. After 330 days of cultivation, the occurrence of pests and diseases and verification of plant mortality were carried out. Among all the pests and diseases observed in this experimental trial, the most prevalent disease regardless of the evaluated genotype was the anthracnose stain “Colletotrichum gloeosporioides” (49.20%) and the less frequent diseases were the resine “Dothiorella ribis” (6.87%) and soft rot “Erwinia carotovora” (2.58%). The only occurrence pest was the cochineal in scales “Diaspis echinocacti” (22.69%). The Texas (V13) and Negro Michoacan (F07) genotypes showed the highest occurrence of pests and diseases, from 50% of the total plants, followed by anthracnose stain and cochineal in scales. On the contrary, the genotypes Tamazunchale (V12) and California (V14) were not affected by any pest or disease. It was observed that the genotypes Nopalea Uruapan (V20) and Blanco San Pedro (V19), had a lower occurrence of pests and diseases, less than 20% of the total plants. The genotypes that presented the highest mortality rate were Texas (V13), Blanco San Pedro (V19) and Polotitlan (V09), with 80, 70 and 65% mortality rate, respectively. The genotypes Nopalea Uruapan (V20) and California (V14) had the lowest mortality rate (20 and 35%), respectively. The genotypes that were least affected by pests and diseases and had the lowest mortality rate are Tamazunchale (V12), California (V14) and Nopalea Uruapan (V20)

Nutritive Value, In Vitro Fermentation, and Methane Production of Cactus Cladodes, Sugarcane Bagasse, and Urea

Simple SummaryCyclical droughts affecting arid and semiarid regions promote direct negative impacts on agriculture, with deficits of water availability for the maintenance of crops destined for human supply and animal production, with direct and indirect socioeconomic effects. Although livestock rearing is one of the few viable economic activities for these regions, forage production in terms of quantity and quality is a critical obstacle to support the herd over the year. Research was developed to find a forage adapted to these climates. Cactus cladodes have been used as a traditional ingredient in ruminant diets in dry areas as a solution to forage scarcity. Many traditionally used sources of forage, fresh or conserved, can be added to diets with cactus. However, the limiting factor to their inclusion would be market availability and price. This study showed that cactus cladodes associated with sugarcane bagasse (a high available crop residue) present the same nutritive value of conserved crops as silages and hay. Cactus (Opuntia and Nopalea) are essential for ruminant production systems in arid and semiarid regions due to the limitation of forage production caused by the low precipitation.This study evaluated the effect of different roughages fed to sheep on nutrient and water intake, ingestive behavior, nitrogen balance, microbial protein synthesis, fermentation parameters, and methane production using an in vitro gas production system. The treatments consisted of five diets: cactus cladodes Nopalea (NUB) and Opuntia (OUB), both with the addition of sugarcane bagasse (SB) and urea/ammonium sulfate (urea/as); Tifton hay (TH); corn silage (CS); and sorghum silage (SS), also with added urea/as. The NUB provided greater (p <= 0.03) intakes of dry matter (1024 g/d), digestible organic matter (670 g/d), and crude protein (161 g/d) than those on the SS. The NUB provided greater (p < 0.01) dietary water intake (3023 g/d) than TH. The time spent on rumination was shorter (p < 0.01), and the idle time was longer in animals fed NUB and OUB than TH and CS. Microbial protein synthesis was not affected (p = 0.27). The final pH (6.4) of the incubation fluid and the concentration of NH3-N (39.05 mg/dL) were greater for NUB and OUB. Ruminal parameters and methane production were little or not affected by tested forages. We recommend using cactus cladodes in combination with sugarcane bagasse and urea/as in sheep diets

The Effect of Nopalea™, a Nutritional Supplement Containing Cactus Fruit Juice, on C-Reactive Protein Levels in Healthy Adults Assessed using a Randomized, Double-Blind, and Controlled Experimental Design

This study was developed to investigate the anti-inflammatory effects of a commercial product (Nopalea™) containing Prickly Pear Cactus Fruit Juice in 286 healthy adults. The research method employed was a double blind, placebo controlled, and time-series design with C-Reactive Protein (CRP) serving as a marker for inflammation. CRP levels were measured at baseline as well as at eight weeks and 12 weeks post-treatment

In vitro antimicrobial activity, total polyphenols and flavonoids contents of Nopalea cochenillifera (L.) Salm-Dyck (Cactaceae)

This study evaluated the antimicrobial activity in vitro qualitative and quantitative methods, and made the determination of total polyphenols and flavonoids in the ethanol extract of Nopalea cochenillifera. The assessment determined the antimicrobial minimum inhibitory concentration (MIC) against Escherichia coli, Salmonella typhi, Micrococcus, Klebsiella pneumoniae, Staphylococcus aureus, Candida albicans, Candida glabrata, Prototheca zopffi, Cryptococcus neoformans, Saccharomyces cervisiae e Malassezia furfur. The determination of total polyphenols and flavonoids were significant when compared respectively to the standards of gallic acid and rutin

Cactus Cladodes Opuntia or Nopalea and By-Product of Low Nutritional Value as Solutions to Forage Shortages in Semiarid Areas

Simple Summary In the different livestock production systems, forage is the main feed resource. However, the availability and quality of the forage fluctuate throughout the year due to variable environmental conditions, such as temperature, humidity, location, or lack of rainfall. In semiarid regions, this fact is even more critical. The option for forage plants adapted to the semiarid climate, such as cactus cladodes, becomes indispensable for the sustainability of the systems. Nonetheless, it is necessary to combine the cactus with high-fiber-content feeds (silage, hay, and agroindustry residues, among others) to increase fiber contents in the diet to promote ideal rumen conditions. Based on the knowledge that cactus cladodes (Opuntia spp. and Nopalea spp.) are one of the most viable crops in semiarid regions, the association with a by-product rich in NDF proves to be a more feasible alternative in terms of price and availability, with the producer making the final decision. We aimed to evaluate the effect of the cactus cladodes Nopalea cochenillifera (L). Salm-Dyck. (NUB) and cactus cladodes Opuntia stricta (Haw.) Haw. (OUB), both combined with sugarcane bagasse (SB) plus urea, Tifton hay (TH), corn silage (CS), and sorghum silage (SS) plus urea on nutrient intake and digestibility, ruminal dynamics, and parameters. Five male sheep, fistulated in the rumen, were assigned in a 5 x 5 Latin square design. The NUB provided a higher intake of dry matter (DM) and any nutrients than SS. TH provided larger pools of DM and iNDF. The OUB and CS provided a higher DM degradation. CS provided a higher NDF degradation rate. OUB provided a lower ruminal pH. Depending on the collection time, the lowest pH value was estimated at 3.79 h after the morning feeding. There was an interaction between treatments and collection time on VFA concentrations. Due to the high degradation rate, greater energy intake, less change in rumen pH, greater volatile fatty acid production, and feasibility, we recommend using cactus associated with sugarcane bagasse plus urea in sheep diets

Production of Spineless Cactus in Brazilian Semiarid

The term “spineless cactus” is used in Brazil to designate cultivars of Opuntia ficus indica Mill and Nopalea cochenillifera Salm Dyck. The spineless cactus was consolidated in Brazilian semiarid as a strategic fundamental food resource in several production livestock systems, constituting a plant with enormous productive potential. Thus, the spineless cactus has been widely cultivated and used for several decades, by enabling the animal feeding in critical periods of year because of its characteristics, morpho‐anatomical and physiological (CAM), which makes it tolerant to long droughts, being a crop that presents high productivity in droughts conditions, when compared to other forages. Nevertheless, the spineless cactus is a crop relatively picky about soil and climate characteristics of region, presenting greater growth in fertile soils, as well as in regions where nighttime temperatures are cool and the air humidity is relatively high. Although the crop be adapted to long droughts periods, many times it’s necessary to perform irrigation in its production system, mainly in regions of low rainfall, for to supply its water needs, thus ensuring productivity and survival of crop. Therefore, the knowledge of characteristics of plant, as well as of appropriate management techniques to crop, is essential for the good performance of spineless cactus

Production and selection of accessions of Opuntia spp. with resistance to false carmine cochineal

The genetic improvement program for Opuntia spp. aims to select new genotypes to meet the demands of rural producers in the Brazilian Semiarid region and to expand the genetic base of the crop. The aim of this study was to select accessions of Opuntia spp. with foraging potential and resistance to false cochineal carmine. The research was carried out at the Active Germplasm Bank (BAG) of Opuntia spp. of Paraíba Company Research, Rural Extension and Land Regularization (EMPAER), Pendência Experimental Station, Soledade, Paraíba, Brazil. Accessions were cultivated in rainfed and evaluated in August 2019, 12 months after the implementation of the BAG. The measurement of the Total Green Mass weight of the plants of 121 accessions of the BAG was carried out and the productivity (ton/ha-1) was estimated in a dense cultivation of 100 thousand plants/ha-1. The accessions of Opuntia spp. nº 10, 110, 115 and 119 have growing potential in the Brazilian Semiarid region, as a function of productivity (532.6, 118, 164 and 481 tons/ha-1, respectively) and resistance to false carmine cochineal, in addition to genetic divergence (specific agronomic characteristics) in relation to currently cultivated varieties.The genetic improvement program for Opuntia spp. aims to select new genotypes to meet the demands of rural producers in the Brazilian Semiarid region and to expand the genetic base of the crop. The aim of this study was to select accessions of Opuntia spp. with foraging potential and resistance to false cochineal carmine. The research was carried out at the Active Germplasm Bank (BAG) of Opuntia spp. of Paraíba Company Research, Rural Extension and Land Regularization (EMPAER), Pendência Experimental Station, Soledade, Paraíba, Brazil. Accessions were cultivated in rainfed and evaluated in August 2019, 12 months after the implementation of the BAG. The measurement of the Total Green Mass weight of the plants of 121 accessions of the BAG was carried out and the productivity (ton/ha-1) was estimated in a dense cultivation of 100 thousand plants/ha-1. The accessions of Opuntia spp. nº 10, 110, 115 and 119 have growing potential in the Brazilian Semiarid region, as a function of productivity (532.6, 118, 164 and 481 tons/ha-1, respectively) and resistance to false carmine cochineal, in addition to genetic divergence (specific agronomic characteristics) in relation to currently cultivated varieties