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Analysis of Super-resolution Single Molecule Localization Microscopy Data: a tutorial
The diffraction of light imposes a fundamental limit on the resolution of
light microscopes. This limit can be circumvented by creating and exploiting
independent behaviors of the sample at length scales below the diffraction
limit. In super-resolution single molecule localization microscopy (SMLM), the
independence arises from individual fluorescent labels stochastically switching
between dark and fluorescent states, which in turn allows the pinpointing of
fluorophores post experimentally using a sequence of acquired sparse image
frames. Finally, the resulting list of fluorophore coordinates is utilized to
produce high resolution images or to gain quantitative insight into the
underlying biological structures. Therefore, image processing and
post-processing are essential stages of SMLM. Here, we review the latest
progress on SMLM data processing and post-processing