Tracking nitrogen losses in a greenhouse crop rotation experiment in North China using the EU-Rotate_N simulation model

Vegetable production in China is associated with high inputs of nitrogen, posing a risk of losses to the environment. Organic matter mineralisation is a considerable source of nitrogen (N) which is hard to quantify. In a two-year greenhouse cucumber experiment with different N treatments in North China, non-observed pathways of the N cycle were estimated using the EU-Rotate_N simulation model. EU-Rotate_N was calibrated against crop dry matter and soil moisture data to predict crop N uptake, soil mineral N contents, N mineralisation and N loss. Crop N uptake (Modelling Efficiencies (ME) between 0.80 and 0.92) and soil mineral N contents in different soil layers (ME between 0.24 and 0.74) were satisfactorily simulated by the model for all N treatments except for the traditional N management. The model predicted high N mineralisation rates and N leaching losses, suggesting that previously published estimates of N leaching for these production systems strongly underestimated the mineralisation of N from organic matter

A reconnaissance geochemical drainage survey of the Criffel-Dalbeattie granodiorite complex and its environs

Regional geochemical reconnaissance by stream sediments and panned concentrates was undertaken over an area of 850 km 2 of south-west Scotland. Rocks of Ordovician to Permian age are exposed within the area but the major part is occupied by the Criffel-Dalbeattie granodiorite complex. Multi-element analysis of the samples collected demonstrates patterns of trace element distribution related to the geology and Broad-scale patterns in the distribution of some elements reflect compositional anumber of mineralisation. variations in both the Lower Palaeozoic turbidite sequence and the Criffel- Dalbeattie granodiorite complex, both of which may be sub-divided into specific units on the basis of the geochemical data. The follow-up of copper anomalies in drainage samples from the Black Stockarton Moor area led to the discovery of porphyry-style copper mineralisation within an arcuate belt at least 5 km by 1 km within the Black Stockarton Moor subvolcanic complex and of related disseminated copper mineralisation at Screel Burn. The area to the west of the Criffel-Dalbeattie plutonic complex is also characterised by relatively high boron levels in stream sediments reflecting the widespread occurrence of tourmaline both in association with and peripheral to the copper mineralisation. Vein mineralisation, usually containing baryte in addition to base.metals, is also identifiable from the drainage survey at the eastern margin of the Criffel-Dalbeattie granodiorite; in association with the Lower Carboniferous rocks along the Solway coast; and within the Lower Palaeozoic turbidites in the west of the area

Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation

Previous studies have shown that exogenous ATP (>1µM) prevents bone formation in vitro by blocking mineralisation of the collagenous matrix. This effect is thought to be mediated via both P2 receptor-dependent pathways and a receptor-independent mechanism (hydrolysis of ATP to produce the mineralisation inhibitor pyrophosphate, PPi). Osteoblasts are also known to release ATP constitutively. To determine whether this endogenous ATP might exert significant biological effects, bone-forming primary rat osteoblasts were cultured with 0.5-2.5U/ml apyrase (which sequentially hydrolyses ATP to ADP to AMP + 2Pi). Addition of 0.5U/ml apyrase to osteoblast culture medium degraded extracellular ATP to <1% of control levels within 2 minutes; continuous exposure to apyrase maintained this inhibition for up to 14 days. Apyrase treatment for the first 72 hours of culture caused small decreases (≤25%) in osteoblast number, suggesting a role for endogenous ATP in stimulating cell proliferation. Continuous apyrase treatment for 14 days (≥0.5U/ml) increased mineralisation of bone nodules by up to 3-fold. Increases in bone mineralisation were also seen when osteoblasts were cultured with the ATP release inhibitors, NEM and brefeldin A, as well as with P2X1 and P2X7 receptor antagonists. Apyrase decreased alkaline phosphatase (TNAP) activity by up to 60%, whilst increasing the activity of the PPi-generating ecto-nucleotide pyrophosphatase/phosphodiesterases (NPPs) up to 2.7-fold. Both collagen production and adipocyte formation were unaffected. These data suggest that nucleotides released by osteoblasts in bone could act locally, via multiple mechanisms, to limit mineralisation

Stratabound arsenic and vein antimony mineralisation in Silurian greywackes at Glendinning, south Scotland

Stratiform and disseminated pyrite-arsenopyrite concentrations are overprinted by fracturecontrolled polymetallic mineralisation including stibnite through at least tens of metres of Silurian sediments at Glendinning, near Langholm. Three shallow boreholes were drilled on an anomaly defined by VLF-EM and II? surveys and by antimony values X0 ppm in thin B-C horizon soils. A parallel conductive zone with an accompanying soil anomaly but lacking an IP response was investigated by a fourth hole. The stratabound sulphides form disseminations and bands parallel to the bedding and are particularly concentrated in intraformational breccia units regarded as debris flows, which, together with the presence of smaIl scale slump folds in the greywackes, testify to the existence of an unstable slope during sedimentation. The thickest such unit has a true thickness of 4 m and together with 8 m of adjoining greywackes grades 0.7% As. Phases of fracture-controlled Fe-As-Sb-Pb- Zn-Cu-(?)Hg mineralisation associated with widespread dolomite and quartz veinlets and narrow breccia veins are superimposed on the stratabound mineralisation. Their spatial association with the stratabound mineralisation, the presence of up to 0.33% Sb in the stratiform arsenopyrite and as much as 5% As in the stratiform pyrite, favour a common source for the arsenic and antimony. This source was probably a synsedimentary metal accumulation in a mid or lower fan environment where euxinic conditions periodically developed

Report on geophysical and geological surveys at Blackmount, Argyllshire

Blackmount, on the southern fringe of Rannoch Moor is largely drift covered but, where exposed, the underlying Precambrian Moine psammite contains granitic veins which probably stem from the adjacent (Devonian) Moor of Rannoch granite. These veins are generally pyritiferous and, at one locality, carry small amounts of molybdenite. Blackmount is also traversed by the Ericht-Laidon Fault, which, in theory, and by analogy with a similar fault to the south-east (the Tyndrum Fault), could be a site of significant sulphide mineralisation. Magnetic, very low frequency electro-magnetic (VLF EM), slingram EM and induced polarisation measurements carried out in the area of the veins suggest that the mineralisation has little or no lateral or depth continuation. Similar surveys were successful in locating the Ericht-Laidon Fault beneath drift, but suggest no associated mineralisation down to the\ud greatest depth investigated

Metal sources for the Katanga Copperbelt deposits (DRC) insights from Sr and Nd isotope ratios

The ore deposits of the Central African Copperbelt formed during a multiphase mineralisation process. The basement underlying the Neoproterozoic Katanga Supergroup that hosts the ore, demonstrates the largest potential as metal source. Various ore deposits that formed during different mineralisation phases are taken as case studies, i.e. Kamoto, Luiswishi, Kambove West, Dikulushi and Kipushi (Democratic Republic of Congo, DRC). The Sr and Nd isotopic compositions of gangue carbonates associated with these deposits is determined and compared with those of rocks from several basement units, bordering or underlying the Copperbelt, to infer the metal sources. The mineralising fluid of diagenetic stratiform Cu-Co mineralisation interacted with felsic basement rocks underlying the region. The Co from these deposits is most likely derived from mafic rocks, but this is not observed in the isotopic signatures. Syn-orogenic, stratabound Cu-Co mineralisation resulted mainly from remobilisation of diagenetic sulphides. A limited, renewed contribution of metals from felsic basement rocks might be indicated by the isotope ratios in the western part of the Copperbelt, where the metamorphic grade is the lowest. The mineralising fluid of syn- and post-orogenic, vein-type mineralisations interacted with local mafic rocks, and with felsic basement or siliciclastic host rocks

Regional geochemical and geophysical surveys in the Berwyn Dome and adjacent areas, north Wales

This report describes stream sediment and gravity surveys carried out across the Berwyn Dome and adjacent areas. The gravity survey confirmed the presence of a broad regional Bouguer anomaly low in the central part of the Dome, on which is superimposed several smaller irregular highs and lows. Some of these local anomalies possibly reflect small igneous bodies but more detailed gravity surveys would be needed to determine their form. Near Corwen the Bryneglwys Fault coincides with a 4.5 mGa1 anomaly but southwards the two features diverge, suggesting that the density interface is related either to a splay fault or to the eastern margin of the Lower Palaeozoic Montgomery trough. Some other structural trends are weakly reflected on the Bouguer anomaly and aeromagnetic maps, but there is no clear correlation with known base metal mineralisation. The Bouguer anomalies cannot be attributed to particular structures with any certainty but are probably due to a number of factors, including variation in the Precambrian basement and changes in the lithology and thickness of Lower Palaeozoic sedimentary rocks. There is no evidence for a large granitic body in Lower Palaeozoic rocks underlying the mineralisation at Llangynog. The aeromagnetic map suggests the presence of a magnetic basement at a depth of 3-4 km centred beneath the northwestern margin of the Dome. The stream sediment survey involved the collection of a - 100 mesh stream sediment, panned concentrate and water sample from each of the 399 sites sampled. The sample density was 1 site per 1.5 km*. Cu, Pb, Zn, Ba, Fe, Mn, Co, V, Cr, Ni, Zr, MO and Sn were determined in the stream sediments, Cu, Pb, Zn, Ba, Fe, Mn, Ce, Sn, Sb, Ti, Ni and As in the panned concentrates and Cu, Pb and Zn in stream waters. Major variations in the results are related to (i) hydrous oxide precipitation processes, (ii) contamination from human activities, (iii) base metal and baryte mineralisation, (iv) monazite concentrations in panned concentrates, (v) hitherto unrecorded gold mineralisation and (vi) lithological variations. The latter were related principally to shale-sandstone variation, but groups of elements attributable to the presence of basic intrusions, phosphatic rocks, coal measures, sandstones, limestones and volcanics were also discerned. Threshold levels were established from cumulative frequency curve analysis, and some anomalous sites were examined in the field. Anomalies did not form prominent coherent groups and were generally weak and scattered, with a wide variety of element groupings reflecting a range of causes. Many anomalous panned concentrates were examined mineralogically to try to . determine whether anomalies were related to chemically extreme background lithologies, contamination, or mineralisation. All the anomalies were related to one or more of the major causes of variation, although because of the very limited amount of follow-up work carried out the precise cause of many anomalies remains uncertain. No anomaly is considered to represent a strong prospect but several deserve further limited investigation, notably those associated with (i) gold mineralisation in the northwest of the area, (ii) baryte, perhaps accompanied by base metal ’ mineralisation, associated with Caradocian volcanics and phosphatic rocks at several localities, (iii) mineralisation associated with Llandeilian limestones and volcanic rocks north of Llanrhaeadr, and (iv) copper mineralisation associated with intrusives near the eastern margin of the Dome, where survey data is most incomplete

A mineral reconnaissance survey of the Abington-Biggar-Moffat-area, south-central Scotland

Panned heavy mineral concentrates, mostly obtained from stream sediments, were collected from 195 sites in the Abington-Biggar-Moffat area. The project area, which lies immediately east of the formerly important mining district of Wanlockhead-Leadhills, covers approximately 500 km2 of the north-central sector of the Southern Uplands. It incorporates the Hart Fell range of hills, the headwaters catchment for the River Tweed and River Annan, some tributaries of the River Clyde, and, to the east, the Culter Water, Talla Reservoir, Megget Water and the head of the Ettrick valley. Numerous new occurrences of lead, zinc, copper and barium minerals were found and nine areas are recommended for further investigation. Minor amounts of baryte and traces of cupriferous pyrite were identified in the basal breccia of the-New Red Sandstone deposits in Annandale. The mercury mineral, cinnabar, was identified for the first time in Scotland, occurring in trace amounts in stream sediment concentrates in the Coulter area close to the Southern Upland Fault. Chromiferous spine1 was recognised as a major constituent in the majority of panned samples. It is present as a detrital mineral in greywackes but must have been originally derived from ultrabasic rocks. An unusual mineral widely dispersed in trace amounts is corundum (including some gem-quality sapphire). Historical references (Lauder Lindsay, 1868-9, 1871) to a wide distribution for particle gold were confirmed and many new occurrences found. A local provenance for the element is now considered certain. Some placer concentration of gold and chromiferous spine1 is likely in the alluvium of the valleys of the River Tweed and the Megget Water. Six greywacke formations, previously defined in other parts of the Southern Uplands, were mapped in the project area, each distinguished by a characteristic lithology and heavy mineral content

Living roots magnify the response of soil organic carbon decomposition to temperature in temperate grassland.

Increasing atmospheric carbon dioxide (CO2) concentration is both a strong driver of primary productivity and widely believed to be the principal cause of recent increases in global temperature. Soils are the largest store of the world's terrestrial C. Consequently, many investigations have attempted to mechanistically understand how microbial mineralisation of soil organic carbon (SOC) to CO2 will be affected by projected increases in temperature. Most have attempted this in the absence of plants as the flux of CO2 from root and rhizomicrobial respiration in intact plant-soil systems confounds interpretation of measurements. We compared the effect of a small increase in temperature on respiration from soils without recent plant C with the effect on intact grass swards. We found that for 48 weeks, before acclimation occurred, an experimental 3 °C increase in sward temperature gave rise to a 50% increase in below ground respiration (ca.0.4 kg C m−2; Q10=3.5), whereas mineralisation of older SOC without plants increased with a Q10 of only 1.7 when subject to increases in ambient soil temperature. Subsequent 14C dating of respired CO2 indicated that the presence of plants in swards more than doubled the effect of warming on the rate of mineralisation of SOC with an estimated mean C age of ca.8 y or older relative to incubated soils without recent plant inputs. These results not only illustrate the formidable complexity of mechanisms controlling C fluxes in soils, but also suggest that the dual biological and physical effects of CO2 on primary productivity and global temperature have the potential to synergistically increase the mineralisation of existing soil C

Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity

Previous work has shown that acidosis prevents bone nodule formation by osteoblasts in vitro by inhibiting mineralisation of the collagenous matrix. The ratio of phosphate (Pi) to pyrophosphate (PPi) in the bone microenvironment is a fundamental regulator of bone mineralisation. Both Pi and PPi, a potent inhibitor of mineralisation, are generated from extracellular nucleotides by the actions of ecto‐nucleotidases. This study investigated the expression and activity of ecto‐nucleotidases by osteoblasts under normal and acid conditions. We found that osteoblasts express mRNA for a number of ecto‐nucleotidases including NTPdase 1–6 (ecto‐nucleoside triphosphate diphosphohydrolase) and NPP1‐3 (ecto‐nucleotide pyrophosphatase/phosphodiesterase). The rank order of mRNA expression in differentiating rat osteoblasts (day 7) was Enpp1 > NTPdase 4 > NTPdase 6 > NTPdase 5 > alkaline phosphatase > ecto‐5‐nucleotidase > Enpp3 > NTPdase 1 > NTPdase 3 > Enpp2 > NTPdase 2. Acidosis (pH 6.9) upregulated NPP1 mRNA (2.8‐fold) and protein expression at all stages of osteoblast differentiation compared to physiological pH (pH 7.4); expression of other ecto‐nucleotidases was unaffected. Furthermore, total NPP activity was increased up to 53% in osteoblasts cultured in acid conditions (P < 0.001). Release of ATP, one of the key substrates for NPP1, from osteoblasts, was unaffected by acidosis. Further studies showed that mineralised bone formation by osteoblasts cultured from NPP1 knockout mice was increased compared with wildtypes (2.5‐fold, P < 0.001) and was partially resistant to the inhibitory effect of acidosis. These results indicate that increased NPP1 expression and activity might contribute to the decreased mineralisation observed when osteoblasts are exposed to acid conditions