3D characterization of bone strains in the rat tibia loading model

Bone strain is considered one of the factors inducing bone tissue response to loading. Nevertheless, where animal studies can provide detailed data on bone response, they only offer limited information on experimental bone strains. Including micro-CT-based finite element (micro FE) models in the analysis represents a potent methodology for quantifying strains in bone. Therefore, the main objective of this study was to develop and validate specimen-specific micro FE models for the assessment of bone strains in the rat tibia compression model. Eight rat limbs were subjected to axial compression loading; strain at the medio-proximal site of the tibiae was measured by means of strain gauges. Specimen-specific micro FE models were created and analyzed. Repeated measurements on each limb indicated that the effect of limb positioning was small (COV= 6.45 ± 2.27 %). Instead, the difference in the measured strains between the animals was high (54.2%). The computational strains calculated at the strain gauge site highly correlated to the measured strains (R 2=0.95). Maximum peak strains calculated at exactly 25% of the tibia length for all specimens were equal to 435.11 ± 77.88 microstrains (COV=17.19%). In conclusion, we showed that strain gauge measurements are very sensitive to the exact strain gauge location on the bone; hence, the use of strain gauge data only is not recommended for studies that address at identifying reliable relationships between tissue response and local strains. Instead, specimen-specific micro FE models of rat tibiae provide accurate estimates of tissue-level strain

Bone mechanical properties in healthy and diseased states

The mechanical properties of bone are fundamental to the ability of our skeletons to support movement and to provide protection to our vital organs. As such, deterioration in mechanical behavior with aging and/or diseases such as osteoporosis and diabetes can have profound consequences for individuals’ quality of life. This article reviews current knowledge of the basic mechanical behavior of bone at length scales ranging from hundreds of nanometers to tens of centimeters. We present the basic tenets of bone mechanics and connect them to some of the arcs of research that have brought the field to recent advances. We also discuss cortical bone, trabecular bone, and whole bones, as well as multiple aspects of material behavior, including elasticity, yield, fracture, fatigue, and damage. We describe the roles of bone quantity (e.g., density, porosity) and bone quality (e.g., cross-linking, protein composition), along with several avenues of future research.Author manuscrip

Vsini-s for late-type stars from spectral synthesis in K-band region

We analyse medium-resolution spectra (R\sim 18000) of 19 late type dwarfs in order to determine vsini-s using synthetic rather than observational template spectra. For this purpose observational data around 2.2 $\mu$m of stars with spectral classes from G8V to M9.5V were modelled. We find that the Na I (2.2062 and 2.2090 $\mu$m) and $^{12}$CO 2-0 band features are modelled well enough to use for vsini determination without the need for a suitable observational template spectra. Within the limit of the resolution of our spectra, we use synthetic spectra templates to derive vsini values consistent with those derived in the optical regime using observed templates. We quantify the errors in our vsini determination due to incorrect choice of model parameters \Teff, log $g$, $v_{\rm micro}$, [Fe/H] or FWHM and show that they are typically less than 10 per cent. We note that the spectral resolution of our data(\sim 16 km/s) limited this study to relatively fast rotators and that resolutions of 60000 will required to access most late-type dwarfs.Comment: 8 pages, 4 figures, 3 tables, accepted to the MNRA

Iron and zinc grain density in common wheat grown in Central Asia

Sixty-six spring and winter common wheat genotypes from Central Asian breeding programs were evaluated for grain concentrations of iron (Fe) and zinc (Zn). Iron showed large variation among genotypes, ranging from 25 mg kg1 to 56 mg kg1 (mean 38 mg kg1). Similarly, Zn concentration varied among genotypes, ranging between 20 mg kg1 and 39 mg kg1 (mean 28 mg kg1). Spring wheat cultivars possessed higher Fe-grain concentrations than winter wheats. By contrast, winter wheats showed higher Zn-grain concentrations than spring genotypes. Within spring wheat, a strongly significant positive correlation was found between Fe and Zn. Grain protein content was also significantly (P < 0.001) correlated with grain Zn and Fe content. There were strong significantly negative correlations between Fe and plant height, and Fe and glutenin content. Similar correlation coefficients were found for Zn. In winter wheat, significant positive correlations were found between Fe and Zn, and between Zn and sulfur (S). Manganese (Mn) and phosphorus (P) were negatively correlated with both Fe and Zn. The additive main effects and multiplicative interactions (AMMI) analysis of genotype × environment interactions for grain Fe and Zn concentrations showed that genotype effects largely controlled Fe concentration, whereas Zn concentration was almost totally dependent on location effects. Spring wheat genotypes Lutescens 574, and Eritrospermum 78; and winter wheat genotypes Navruz, NA160/HEINEVII/BUC/3/F59.71//GHK, Tacika, DUCULA//VEE/MYNA, and JUP/4/CLLF/3/II14.53/ODIN//CI13431/WA00477, are promising materials for increasing Fe and Zn concentrations in the grain, as well as enhancing the concentration of promoters of Zn bioavailability, such as S-containing amino acids

Bending and springback prediction method based on multi-scale finite element analyses for high bendability and low springback sheet generation

In this study, a sheet bendability and springback property evaluation technology through bending test simulations is newly developed using our multi-scale finite element analysis code, which is based on the crystallographic homogenization method

Strain-rate Dependence for Ni/Al Hybrid Foams

Shock absorption often needs stiff but lightweight materials that exhibit a large kinetic energy absorption capability. Open-cell metal foams are artificial structures, which due to their plateau stress, including a strong hysteresis, can in principle absorb large amounts of energy. However, their plateau stress is too low for many applications. In this study we use highly novel and promising Ni/Al hybrid foams which consist of standard, open-cell aluminium foams, where nanocrystalline nickel is deposited by electrodeposition as coating on the strut surface. The mechanical behaviour of cellular materials, also under higher strain-rates, is governed by their micro-structure due to the properties of the strut material, pore/strut geometry and mass distribution over the struts. Micro inertia effects are strongly related to the micro structure. For a conclusive model the exact real micro-structure is needed. In this study a micro-focus computer tomography (μCT) system has been used for the analysis of the micro-structure of the foam samples and for the development of a micro-structural Finite Element (micro-FE) mesh. The micro-structural FE models have been used to model the mechanical behaviour of the Ni/Al hybrid foams under dynamic loading conditions. The simulations are validated by quasi-static compression tests and dynamic split Hopkinson pressure bar tests.JRC.G.4-European laboratory for structural assessmen

Homogenization-based multiscale crack modelling: from micro-diffusive damage to macro-cracks

The existence of a representative volume element (RVE) for a class of quasi-brittle materials having a random heterogeneous microstructure in tensile, shear and mixed mode loading is demonstrated by deriving traction–separation relations, which are objective with respect to RVE size. A computational homogenization based multiscale crack modelling framework, implemented in an FE2 setting, for quasi-brittle solids with complex random microstructure is presented. The objectivity of the macroscopic response to the micro-sample size is shown by numerical simulations. Therefore, a homogenization scheme, which is objective with respect to macroscopic discretization and microscopic sample size, is devised. Numerical examples including a comparison with direct numerical simulation are given to demonstrate the performance of the proposed method.Peer ReviewedPostprint (author's final draft

Trabecular fracture zone might not be the higher strain region of the trabecular framework

Trabecular bone fracture is a traumatic and localized event studied worldwide in order to predict it. During the years, researchers focused over the mechanical characterization of the trabecular tissue to understand its mechanics. Several studies pointed out the very local nature of the trabecular failure, finally identifying the fracture zone with the aim to study it separately. The complexity of the three-dimensional trabecular framework and the local nature of the fracture event do not allow the direct evaluation of a single trabecula’s behavior within its natural environment. For this reason, micro-Finite Element Modeling has been seen as the best way to investigate this biomechanical issue. Mechanical strain analysis is adopted in the literature for the identification of micro fracture using criteria based on principal strains. However, it was never verified if the fracture zone is actually the zone where principal strains are concentrated. Here, we show how the maximum strain of the tissue might not be directly correlated to the fracture. In the present work, a previously validated technique was used to identify the fracture zone of 10 trabecular specimen mechanically tested in compression and scanned in micro-CT before and after the mechanical test. Before-compression datasets were used to develop 10 micro-FE models were the same boundary conditions of the mechanical test were reproduced. Our results show how the known linear behavior of the trabecular framework might not be directly related to the development of the fracture suggesting other non-linear phenomenon, like buckling or microdamage, as actual cause of the traumatic event. This result might have several implications both in micro-modeling and in clinical applications for the study of fracture related pathology, like osteoporosis.The micro-CT datasets were produced by Laboratorio di Tecnologia Medica, Istituto Ortopedico Rizzoli, Bologna, Italy, with the financial support of the EU project LHDL (IST-2004-026932). This work was partially supported by the Spanish Government (project number RYC—2015-18888) and by Chair QUAES-UPF Computational Technologies for Healthcare