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Theoretical Perspectives on Protein Folding
Understanding how monomeric proteins fold under in vitro conditions is
crucial to describing their functions in the cellular context. Significant
advances both in theory and experiments have resulted in a conceptual framework
for describing the folding mechanisms of globular proteins. The experimental
data and theoretical methods have revealed the multifaceted character of
proteins. Proteins exhibit universal features that can be determined using only
the number of amino acid residues (N) and polymer concepts. The sizes of
proteins in the denatured and folded states, cooperativity of the folding
transition, dispersions in the melting temperatures at the residue level, and
time scales of folding are to a large extent determined by N. The consequences
of finite N especially on how individual residues order upon folding depends on
the topology of the folded states. Such intricate details can be predicted
using the Molecular Transfer Model that combines simulations with measured
transfer free energies of protein building blocks from water to the desired
concentration of the denaturant. By watching one molecule fold at a time, using
single molecule methods, the validity of the theoretically anticipated
heterogeneity in the folding routes, and the N-dependent time scales for the
three stages in the approach to the native state have been established. Despite
the successes of theory, of which only a few examples are documented here, we
conclude that much remains to be done to solve the "protein folding problem" in
the broadest sense.Comment: 48 pages, 9 figure
A Comparative Study of Disordered and Ordered Protein Folding Dynamics Using Computational Simulation
Folding protein dynamics has been an area of high interest for quite some
time, especially given the increased focus on the field of Biophysics. Because
folding dynamics occur on such short time scales, empirical techniques
developed for more "static" protein events, such as X-ray crystallography,
nuclear magnetic resonance, and green fluorescent protein (GFP) labelling,
aren't as applicable. Instead, computational methods must often be used to
simulate these short lived yet highly dynamic events. One such computational
method that is proven to provide much valuable insight into protein folding
dynamics is Molecular Dynamics Simulation (MD Simulation). This simulation
method is both highly computationally demanding, yet highly accurate in its
modelling of a proteins physical behaviour. Besides MD Simulation, simulations
in general are quite applicable in the context of these protein events. For
example, the simple Gillespie algorithm, a computational technique which can be
executed on almost any personal computer, provides quite the robust view into
protein dynamics given its computational simplicity. This paper will compare
the results of two simulations, an MD simulation of a disordered, six-residue,
carcinogenic protein fragment, and a Gillespie algorithm based simulation of an
ordered folding protein: the mathematically identical nature of the Gillespie
algorithm time series of the asymptotically stochastic hyperbolic tangent
dynamics for the wild type predicting the exact behaviour of the carcinogenic
protein system time series will show the computational power simulations
provide for analyzing both disordered and ordered protein systems.Comment: 13 pages, draft 1, 8 figure
Introduction to Protein Folding
While many good textbooks are available on Protein Structure, Molecular Simulations, Thermodynamics and Bioinformatics methods in general, there is no good introductory level book for the field of Structural Bioinformatics. This book aims to give an introduction into Structural Bioinformatics, which is where the previous topics meet to explore three dimensional protein structures through computational analysis. We provide an overview of existing computational techniques, to validate, simulate, predict and analyse protein structures. More importantly, it will aim to provide practical knowledge about how and when to use such techniques. We will consider proteins from three major vantage points: Protein structure quantification, Protein structure prediction, and Protein simulation & dynamics. In this chapter we explore basic physical and chemical concepts required to understand protein folding. We introduce major (de)stabilising factors of folded protein structures such as the hydrophobic effect and backbone entropy. In addition, we consider different states along the folding pathway, as well as natively disordered proteins and aggregated protein states. In this chapter, an intuitive understanding is provided about the protein folding process, to prepare for the next chapter on the thermodynamics of protein folding. In particular, it is emphasized that protein folding is a stochastic process and that proteins unfold and refold in a dynamic equilibrium. The effect of temperature on the stability of the folded and unfolded states is also explained
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