Microbiome profile associated with malignant pleural effusion.

INTRODUCTION:There is ongoing research into the development of novel molecular markers that may complement fluid cytology malignant pleural effusion (MPE) diagnosis. In this exploratory pilot study, we hypothesized that there are distinct differences in the pleural fluid microbiome profile of malignant and non-malignant pleural diseases. METHOD:From a prospectively enrolled pleural fluid biorepository, samples of MPE were included. Non-MPE effusion were included as comparators. 16S rRNA gene V4 region amplicon sequencing was performed. Exact Sequence Variants (ESVs) were used for diversity analyses. The Shannon and Richness indices of alpha diversity and UniFrac beta diversity measures were tested for significance using permutational multivariate analysis of variance. Analyses of Composition of Microbiome was used to identify differentially abundant bacterial ESVs between the groups controlled for multiple hypothesis testing. RESULTS:38 patients with MPE and 9 with non-MPE were included. A subgroup of patients with metastatic adenocarcinoma histology were identified among MPE group (adenocarcinoma of lung origin (LA-MPE) = 11, breast origin (BA-MPE) = 11). MPE presented with significantly greater alpha diversity compared to non-MPE group. Within the MPE group, BA-MPE was more diverse compared to LA-MPE group. In multivariable analysis, ESVs belonging to family S24-7 and genera Allobaculum, Stenotrophomonas, and Epulopiscium were significantly enriched in the malignant group compared to the non-malignant group. CONCLUSION:Our results are the first to demonstrate a microbiome signature according to MPE and non-MPE. The role of microbiome in pleural effusion pathogenesis needs further exploration

Full-field pulsed magneto-photoelasticity – Experimental Implementation

This paper contains a description of the experimental procedure employed when using a pulsed-magneto-polariscope (PMP) and some initial full-field through-thickness measurements of the stress distribution present in samples containing 3D stresses. The instrument uses the theory of magneto-photoelasticity (MPE), which is an experimental stress analysis technique that involves the application of a magnetic field to a birefringent model within a polariscope. MPE was developed for through-thickness stress measurement where the integrated through-thickness birefringent measurement disguises the actual stress distribution. MPE is mainly used in toughened glass where the through-thickness distribution can reduce its overall strength and so its determination is important. To date MPE has been a single-point 2D through-thickness measurement and the analysis time is prohibitive for the investigation of an area which may contain high localised stresses. The pulsed-magneto-polariscope (PMP) has been designed to enable the application of full-field 3D MPE [ ]. Using a proof-of concept PMP several experimental measurements were made, these were promising and demonstrate the potential of the new instrument. Further development of this technique presents several exciting possibilities including a tool for the measurement of the distribution of principal stress difference seen in a general 3D model

Penggunaan modul pembelajaran elektronik (MPE) pengenalan pelancongan H 111 : tinjauan terhadap pensyarah dan pelajar Diploma Pengurusan Pelancongan, Politeknik Johor Bahru

Era Teknologi Maklumat dan Komunikasi (ICT) telah melihat perkembangan penggunaan komputer sebagai alat berpotensi untuk meningkatkan proses pengajaran dan pembelajaran. Oleh itu, kajian ini meninjau Penggunaan Modul Pembelajaran Elektronik (MPE) dalam mata pelajaran Pengenalan Pelancongan H 111 di kalangan pensyarah dan pelajar semester satu Diploma Pengurusan Pelancongan, Politeknik Johor Bahru. Seramai 40 responden yang terdiri daripada pelajar dan tiga orang pensyarah telah dipilih bagi menjawab tiga persoalan kajian yang dinyatakan. Maklum balas melalui soal selidik pelajar telah dianalisis menggunakan perisian Sran'sf/caV Package /or <Sbc;'a/ Aaence (SPSS) versi 11.0, manakala maklum balas soalan terbuka pensyarah pula dipersembahkan dalam bentuk jadual serta penerangan. Dalam persoalan kajian pertama, skor min keseluruhan yang diperolehi terhadap penerimaan pelajar ke atas MPE Pengenalan Pelancongan H i l l yang dihasilkan adalah di tahap baik iaitu 3.74. Manakala persoaian kajian kedua, pelajar turut bersetuju bahawa isi kandungan MPE Pengenalan Pelancongan H i l l dapat meningkatkan tahap pemahaman mereka (skor min keseluruhan 3.654). Seterusnya dalam persoalan kajian ketiga, skor min keseluruhan yang diperolehi adalah 3.634 menunjukkan satu dapatan keputusan yang baik terhadap penggunaan MPE dalam membantu proses pembelajaran yang berkesan. Begitu juga dengan hasil dapatan yang diterima dari pensyarah, mendapati keputusan ketiga-tiga persoalan kajian berada di tahap setuju atau baik. Di akhir bab kajian ini, beberapa pandangan telah dikemukakan untuk mempertingkatkan lagi kecekapan MPE melalui pembangunan perisian yang lebih mantap di masa hadapan

Molecular Gas and Star Formation in the Host Galaxy of I Zw 1

A recent analysis of high angular resolution NIR imaging and spectroscopic data in conjunction with Plateau de Bure interferometric mm-line observations indicate the presence of a circum-nuclear starburst ring of about 1.5 arcsec (1.5 kpc) diameter in I Zw 1. High angular resolution NIR imaging, using the MPE SHARP camera at the ESO NTT, HST V-band images, as well as NIR spectroscopy with MPE 3D provide an improved analysis of the star formation activity in the disk and nucleus of I Zw 1. We present first results from subarcsecond interferometric imaging in the 12CO(2-1) line using the Plateau de Bure Interferometer.Comment: Contributed talk presented at the Joint MPE,AIP,ESO workshop on NLS1s, Bad Honnef, Dec. 1999, to appear in New Astronomy Reviews; also available at http://wave.xray.mpe.mpg.de/conferences/nls1-worksho

Full-field pulsed-magneto-photoelasticity - a description of the instrument

This paper describes a novel instrument used for the analysis of full-field through-thickness stress distributions using the theory of magneto-photoelasticity (MPE) developed by Aben and Clarke et al. [ , , ]. MPE is an experimental stress analysis technique which involves the application of a magnetic field parallel to an electromagnetic wave propagating through a birefringent model within a polariscope. The effect viewed through the polariscope is then a combination of the model’s birefringence and the Faraday rotation created in the model by the magnetic field. Aben developed this technique especially for use in the measurement of stress profiles where the integrated photoelastic pattern alone yields little information. Clarke et al. developed MPE in order to study toughened glass. To date, the technique of MPE has been a single-point measurement and this is of limited utility in the investigation of 3D stress in toughened glasses. The pulsed-magneto-polariscope (PMP), described here, enables the full-field application of MPE. This paper contains a description of the novel apparatus, and demonstrations used to validate the performance of a proof-of-concept PMP instrument. The paper also highlights improvements in the application of MPE which are now possible with this new equipment. These improvements include the extension of MPE to larger areas of analysis, 3D stress analysis and the possibility of analysing a general unknown stress distribution