Methyl jasmonate and cis jasmone induce apoptosis in Pc-3 and LncaP prostate cancer lines

Methyl jasmonate (MJ) and cis jasmone (CJ), lipid-derived plant stress hormones, were studied for their effects against both hormone dependent (LNCaP) and hormone independent (PC3) human prostate cancer cell lines. Both 2 mM MJ or CJ inhibited the growth of the cell lines. An alamar blue assay was used to determine IC50 values for MJ and CJ in both cell lines at 24 hrs, 48 hrs and 72 hrs. At 24 hrs the MJ IC50 values were 2.25 and 2.05 while the CJ values were 3.00 and 1.25 in the PC3 and LNCaP lines respectively. Cell cycle analysis revealed that MJ and CJ induced apoptosis in both cell lines as well as activated caspase-3. An in vitro assay showed that MJ and CJ did not significantly inhibit either 5-lipoxygenase or 15-lipoxygenase. 5-HETE was able to stimulate cell growth in the presence or absence of MJ in both the cell lines

Cyclic peptide production using a macrocyclase with enhanced substrate promiscuity and relaxed recognition determinants

This project was supported by grants from the ERC (no. 339367, MJ), BBSRC IBCatalyst (no. BB/M028526/1, MJ, WEH), BBSRC FoF (no. BB/M013669/1, MJ, WEH), IBioIC Exemplar (no. 2014-2-4, MJ, WEH), an AstraZeneca studentship (MJ, WEH, LT, KR), the Academy of Finland (no. 259505, DPF) and the SULSA leaders award (WEH). The authors like to thank the Aberdeen Proteomics Facility and the Aberdeen School of Natural and Computing Sciences MS Facility for LCMS analysis. Electronic supplementary information (ESI) available: Experimental section, Fig. S1–S60 and Tables S1–S3. See DOI: 10.1039/c7cc05913bPeer reviewedPublisher PD

Primary and successive events in the Madden–Julian Oscillation

Conventional analyses of the MJO tend to produce a repeating cycle, such that any particular feature cannot be unambiguously attributed to the current or previous event. We take advantage of the sporadic nature of the MJO and classify each observed Madden-Julian (MJ) event as either primary, with no immediately preceding MJ event, or successive, which does immediately follow a preceding event. 40% of MJ events are primary events. Precursor features of the primary events can be unambiguously attributed to that event. A suppressed convective anomaly grows and decays in situ over the Indian Ocean, prior to the start of most primary MJ events. An associated mid-tropospheric temperature anomaly destabilises the atmosphere, leading to the generation of the active MJ event. Hence, primary MJ events appear to be thermodynamically triggered by a previous dry period, although stochastic forcing may also be important. Other theories predict that boundary-layer convergence, humidity, propagation of dynamical structures around the Equator, sea surface temperatures, and lateral forcing by extratropical transients may all be important in triggering an event. Although precursor signals from these mechanisms are diagnosed from reanalysis and satellite observational data in the successive MJ events, they are all absent in the primary MJ events. Hence, it appears that these apparent precursor signals are part of the MJO once it is established, but do not play a role in the spontaneous generation of the MJO. The most frequent starting location of the primary events is the Indian Ocean, but over half of them start elsewhere, from the maritime continent to the western Pacific

Main Elements of Logistics

Virtually lossless self-compression of 10-mJ 3.9-um sub-100 fs pulses in bulk YAG resulting in 9-mJ 33-fs pulses is reported. Generated peak power exceeds 250 GW which is suitable for filamentation in ambient air

Tracking Report 2009 MJ Soffe, Jordan 460039453H

This document is part of a digital collection provided by the Martin P. Catherwood Library, ILR School, Cornell University, pertaining to the effects of globalization on the workplace worldwide. Special emphasis is placed on labor rights, working conditions, labor market changes, and union organizing.FLA_2009_MJ_Soffe_TR_Jordan_460039453H.pdf: 11 downloads, before Oct. 1, 2020

Spin dependent inelastic collisions between metastable state two-electron atoms and ground state alkali-atoms

Experimentally the spin dependence of inelastic collisions between ytterbium (Yb) in the metastable 3P0 state and lithium (Li) in the Li ground state manifold is investigated at low magnetic fields. Using selective excitation all magnetic sublevels mJ of 174Yb(3P0) are accessed and four of the six lowest lying magnetic sublevels of 6Li are prepared by optical pumping. On the one hand, mJ-independence of collisions involving Li(F=1/2) atoms is found. A systematic mJ-dependence in collisions with Li(F=3/2) atoms, in particular suppressed losses for stretched collisional states, is observed on the other hand. Further, mJ-changing processes are found to be of minor relevance. The span of observed inelastic collision rates is between 1*10^{-11} cm^3/s and 40*10^{-11} cm^3/s, and a possible origin of the observed behavior is discussed.Comment: 12 pages, 4 figure

Local and ecological food consumption - case studies in Finland and Sweden

Preliminary results show that in the Juva district amount of energy according to the purchase diary is 7.6 MJ/person/day and in Järna district the amount is 9.4 MJ/person/day. Our results are in a range that is reasonable

Carbon-ammonia pairs for adsorption refrigeration applications : ice making, air conditioning and heat pumping

A thermodynamic cycle model is used to select an optimum adsorbent-refrigerant pair in respect of a chosen figure of merit that could be the cooling production (MJ m(-3)), the heating production (MJ m(-3)) or the coefficient of performance (COP). This model is based mainly on the adsorption equilibrium equations of the adsorbent-refrigerant pair and heat flows. The simulation results of 26 various activated carbon-ammonia pairs for three cycles (single bed, two-bed and infinite number of beds) are presented at typical conditions for ice making, air conditioning and heat pumping applications. The driving temperature varies from 80 degrees C to 200 degrees C. The carbon absorbents investigated are mainly coconut shell and coal based types in multiple forms: monolithic, granular, compacted granular, fibre, compacted fibre, cloth, compacted cloth and powder. Considering a two-bed cycle, the best thermal performances based on power density are obtained with the monolithic carbon KOH-AC, with a driving temperature of 100 degrees C; the cooling production is about 66 MJ m(-3) (COP = 0.45) and 151 MJ m(-3) (COP = 0.61) for ice making and air conditioning respectively; the heating production is about 236 MJ m(-3) (COP = 1.50)