Extract of Caesalpinia Sappan L. as Antibacterial Feed Additive on Intestinal Microflora of Laying Quail

Caesalpinia sappan L. is a phytobiotic plant that serves as an antibacterial. Active compound such as flavonoids in the C. sappan L. extract acts as an antibacterial. The protein food sources in Indonesia including poultry such as quail. Several studies demonstrated that quail are very susceptible to bacterial infections including Escherichia coli and Salmonella. Thus C. sappan has been used as food additive for quail in laying eggs. This research objective was to study the effects of C. sappan L. extract against intestinal miclofora of quail in laying eggs. This study used 168 quails with 4 treatments and 5 replications, each treatment used 7 quails. This study used 4 Wood Extract C. sappan L. treatment with different concentrations including 0%, 0.2%, 0.4%, 0.6%. The results showed that administration of Extract Wood C. sappan L. was not significantly different (P> 0.05) to the total number of bacteria E. coli with the average P0 (6.0903 Log CFU), P1 (6.0903 log CFU), P2 (6.0887 Log CFU), and P3 (6.0868 Log CFU). Meanwhile, it was significantly different (P <0.05) to the total number of Salmonella bacteria in the gut laying with the average number of bacteria P0 (5.4059 Log CFU), P1 (5.4048 Log CFU), P2 (5.4045 Log CFU), and P3 (5.4039 Log CFU). It is concluded that flavonoids compounds in C. sappan L. extract could decrease the number of Salmonella, but not E. coli, in the intestine of quail

The Northern Bobwhite Quail, 2011

Data sheet produced by the Iowa Department of Natural Resources is about different times of animals, insects, snakes, birds, fish, butterflies, etc. that can be found in Iowa

Budidaya Puyuh (Coturnix Coturnix Japonica ) Di Pekarangan Sebagai Sumber Protein Hewani Dan Penambah Income Keluarga

Yard can provide great benefits to society, because it can planted vegetables, fruit and herbs such as turmeric, lemongrass etc. Yard can also be used as a source of animal protein and an additional source of family income. The advantages of cultivate quail is it has small body size and weighs around 130 grams, short life cycle, high egg productivity ( in the period peak daily egg production can produce about 90 % of the total population in the cage ), feed intake slightly ( about 20 grams / head / day ), easily cultivated and does not require high investment. Quail can deliver results in the form of daily egg production, meat from culled quail ( quail that had stopped laying eggs or egg productivity plummeted start that after about 8 months old ), as well as produce manure that can be processed into organic fertilizer. Yard with an area of approximately 2 m2 can be used to maintain approximately 250 quail at a cost of approximately Rp.3,887,000. With the average cost of feed per month Rp.375,000, of the quail maintenance can provide net income from egg production per day on average Rp.11,500 and income from the sale of quail has not productived Rp.787,500. Based on the advantages of the quail and the results of the economic analysis showed that the cultivation from quail in the yard can provide family income and improve nutrition

Assembly of the Inner Perivitelline Layer, a Homo log of the Mammalian Zona Pellucida: An Immunohistochemical and Ultrastructural Study

The avian inner perivitelline layer (IPVL), a homologous structure to the mammalian zona pellucida, is deposited between the granulosa cells and the oocyte cell membrane during folliculogenesis. The glycoprotein meshwork of the IPVL forms a 3-dimensional matrix and possesses important functions in the fertilization process: it contributes to the binding of avian spermatozoa to the oocyte and induces acrosomal exocytosis. In contrast to the zona pellucida of mammals, the IPVL does not prevent the physiological polyspermy found in birds. Previous studies have shown that in the Japanese quail (Cotumix japonica) at least 5 glycoproteins are constituents of the IPVL (ZP1, ZP2, ZP3, ZP4, and ZPD). In this study, we investigated the spatiotennporal assembly pattern of the IPVL during folliculogenesis using immunohistochemical and ultrastructural methods. The obtained results clearly show that these glycoproteins are incorporated into the IPVL at distinct points during follicular development, supporting the hypothesis that ZP2 and ZP4 form a type of prematrix into which ZP1, ZP3, and ZPD are integrated at a later stage of development. Copyright (C) 2011 S. Karger AG, Base

Resíduos de aflatoxina B1 em fígado de codornas japonesas (Coturnix japonica) expostas a alimentos contaminados e à corticosterona exógena

The combined effect of aflatoxin B1 (AFB1 ) exposition and stress induced by corticosterone (CORT) upon the residual aflatoxin levels in livers of Japanese quail was examined. A total of 144 Japanese quail (Coturnix japonica) were divided into 6 treatments with 6 replicates per treatment, each containing 2 males and 2 females. The different treatments resulted from the combination of the presence or absence of CORT in drinking water (5 mg/L) with the presence or absence of AFB1 supplementation (100 or 500 ng/g). Dietary treatments were offered from 5 to 11 weeks of age. The different diets were: Treatment (T) 1: basal diet (B) AFB1 (15 ng/g); T2: B plus AFB1 (100 ng/g); T3: B plus AFB1 (500 ng/g); T4: B plus CORT (5 mg/L); T5: B plus AFB1 (100 ng/g) and CORT (5 mg/L); and T6: B plus AFB1 (500 ng/g) and CORT (5 mg/L). The residual levels of AFB1 were remarkably higher in livers of birds fed with AFB1 in comparison with those belonging to the group that received a combination of AFB1 and CORT (P< 0.05). A plausible explanation about the facts that could be responsible for observed reduction in the toxin carryover in liver was suggested. These results are very striking and may constitute the start point for a series of studies that aim to elucidate the precise influence of hypothalamic-pituitary-adrenal axis (HPA) response to stress in birds exposed to AFB.Estudou-se o efeito combinado da exposição à aflatoxina B1 (AFB1 ) e o estresse induzido por corticosterona (CORT) sobre os níveis de residuais de AFB1 em fígados de codornas japonesas. Um total de 144 codornas japonesas (Coturnix japonica) foram divididas em 6 tratamentos com 6 repetições por tratamento, cada uma contendo 2 machos e 2 fêmeas. As dietas dos tratamentos foram oferecidas da 5ª até a 11ª semana de idade. Os diferentes tratamentos resultaram da combinação da presença ou ausência de CORT na água de beber (5 mg / L), com a presença ou ausência de suplementação AFB1 (100 ou 500 ng /g) As dietas diferentes foram: Tratamento (T) 1: dieta basal (B) AFB1 (15 ng/g); T2: B mais AFB1 (100 ng / g); T3: B mais AFB1 (500 ng / g); T4: B mais CORT (5 mg / L); T5: B mais AFB1 (100 ng / g) e CORT (5 mg / L), e T6: B mais AFB1 (500 ng / g) e CORT (5 mg / L). Os níveis residuais de AFB1 foram significativamente mais elevados em fígados de aves alimentadas com AFB1 em comparação com os que pertencem ao grupo que recebeu uma combinação de AFB1 e CORT (P< 0,05). Sugere-se uma possível explicação para a redução dos níveis da toxina observada naqueles animais submetidos a dieta com CORT. Estes resultados constituem-se de um ponto de início para uma série de estudos que visam a elucidar a influência da resposta do eixo hipotalâmico-hipofisário-adrenais (HPA) ao stress em aves expostas a AFB.Fil: Magnoli, Alejandra Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosa, Carlos Alberto da Rocha. Universidade Federal Rural de Rio de Janeiro; BrasilFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marin, Raul Hector. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Stress induced polarization of immune-neuroendocrine phenotypes in Gallus gallus

Immune-neuroendocrine phenotypes (INPs) stand for population subgroups differing in immune-neuroendocrine interactions. While mammalian INPs have been characterized thoroughly in rats and humans, avian INPs were only recently described in Coturnix coturnix (quail). To assess the scope of this biological phenomenon, herein we characterized INPs in Gallus gallus (a domestic hen strain submitted to a very long history of strong selective breeding pressure) and evaluated whether a social chronic stress challenge modulates the individuals’ interplay affecting the INP subsets and distribution. Evaluating plasmatic basal corticosterone, interferon-γ and interleukin-4 concentrations, innate/acquired leukocyte ratio, PHA-P skin-swelling and induced antibody responses, two opposite INP profiles were found: LEWIS-like (15% of the population) and FISCHER-like (16%) hens. After chronic stress, an increment of about 12% in each polarized INP frequency was found at expenses of a reduction in the number of birds with intermediate responses. Results show that polarized INPs are also a phenomenon occurring in hens. The observed inter-individual variation suggest that, even after a considerable selection process, the population is still well prepared to deal with a variety of immune-neuroendocrine challenges. Stress promoted disruptive effects, leading to a more balanced INPs distribution, which represents a new substrate for challenging situations.Fil: Nazar, Franco Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Estevez, Inma. Centro de Investigación. Neiker - Tecnalia; EspañaFil: Correa, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentin

Effects on the reproductive system in domestic dowl (Gallus domesticus) after embryonic exposure to estrogenic substances

Environmental pollutants with estrogenic activity have a potential to disrupt oestrogen-dependent developmental processes. Thus, the aim of this thesis was to investigated how embryonic exposure to the estrogenic pollutants EE2 (17 alpha- ethynyloestradiol) and o,p´-DDT (1-[2-chlorophenyl]-1-[4-chlorophenyl]-2,2,2-trichloro-ethane) affects the reproductive system in the domestic hen (Gallus domesticus). Hens exposed in ovo to 20 or 60 ng EE2/g egg or 37 or 75 micro g o,p´-DDT/g egg produced a normal number of eggs but with thinner shells as adults. These hens also showed a reduction of shell gland capillaries with carbonic anhydrase (CA) activity. CA is considered a key enzyme in shell formation and the developmentally induced disruption of this enzyme may explain the observed eggshell thinning. Left oviduct and infundibulum was shortened in exposed hens and severely malformed in hens exposed to 150 or 300 micro g o,p´-DDT/g egg, which likely explained the inhibited egg production in these birds. Semen output was significantly reduced in both o,p´-DDT and EE2 exposed roosters, whereas sperm quality was unaffected. The left testis was deformed with an atrophied epididymis. Altered plasma inhibin concentration and histological evaluation implied a disturbed Sertoli cell function resulting in reduced germ cell production. Structural malformations in the epididymis indicating disrupted fluid transfer and transport of spermatozoa are possible contributors to the reduced semen output. Exposure to o,p´-DDT resulted in a wider range of effects than EE2 such as feminisation of the cloaca at hatch, cloacal deformation in adult roosters and a reduced comb weight, while hens showed retained right oviducts. Right spur diameter was affected by both substances.Localisation of epididymal progesterone receptors and cytoplasmic and membrane associated oestrogen receptor alfa in late spermatids, spermatozoa and epididymal tissues were novel findings in rooster. Embryonic exposure of domestic fowl to o,p´-DDT or EE2 resulted in persistent malformations of the reproductive organs of both sexes, with eggshell thinning and reduced semen production as a consequence. The results provide a possible explanation for how eggshell thinning may be induced in wild birds and show that also male birds may suffer from reduced reproductive success due to oestrogen-like pollutants

Eggshell pigment composition covaries with phylogeny but not with life history or with nesting ecology traits of British passerines

No single hypothesis is likely to explain the diversity in eggshell coloration and patterning across birds, suggesting that eggshell appearance is most likely to have evolved to fulfill many nonexclusive functions. By controlling for nonindependent phylogenetic associations between related species, we describe this diversity using museum eggshells of 71 British breeding passerine species to examine how eggshell pigment composition and concentrations vary with phylogeny and with life-history and nesting ecology traits. Across species, concentrations of biliverdin and protoporphyrin, the two main pigments found in eggshells, were strongly and positively correlated, and both pigments strongly covaried with phylogenetic relatedness. Controlling for phylogeny, cavity-nesting species laid eggs with lower protoporphyrin concentrations in the shell, while higher biliverdin concentrations were associated with thicker eggshells for species of all nest types. Overall, these relationships between eggshell pigment concentrations and the biology of passerines are similar to those previously found in nonpasserine eggs, and imply that phylogenetic dependence must be considered across the class in further explanations of the functional significance of avian eggshell coloration