Potential of Lactic Acid Bacteria Isolated From Dangke and Indonesian Beef as Hypocholesterolaemic Agent

Lactobacillus fermentum strains were successfully isolated from dangke which was a fresh cheese-like product originating from Enrekang, South Sulawesi Province, Indonesia. In addition, Lactobacillus plantarum and Lactobacillus acidophillus were isolated from beef. This study aimed to investigate the ability of those 8 LAB strains from dangke and beef in lowering cholesterol level by using in vitro study. Strain of Lactic acid bacteria used were L. fermentum strains (A323L, B111K, B323K, C113L, C212L), L. plantarum strains (IIA-1A5 and IIA-2C12), and L. acidophillus IIA-2B4. Variables observed were identification of Bile Salt Hydrolase (BSH) gene by Polymerase Chain Reaction (PCR), BSH activity and cholesterol assimilation. Phylogenetic tree indicated homology of L. plantarum IIA-IA5 was 98% to BSH gene of L. plantarum Lp529 with access code of FJ439771 and FJ439775 obtained from GenBank. The results demonstrated that eight strains of LAB isolated from dangke and beef that potentially showed cholesterol-lowering effects were L. fermentum B111K and L. plantarum IIA-1A5. L. fermentum B111K was able to assimilate cholesterol by 4.10% with assimilated cholesterol of 0.13 mg in 1010 cells. In addition, L. plantarum IIA-1A5 had BSH gene and BSH activity, as well as the ability to assimilate cholesterol by 8.10% with assimilated cholesterol of 0.06 mg in 1010 cells. It is concluded that L. fermentum B111K and L. plantarum IIA-1A5 were strains that showed cholesterol-lowering effects

Screening Lactic Acid Bacteria for Antimicrobial Compound Production

Lactic Acid Bacteria was known as potential probiotic used in food industries and dairy products and probable to produce antimicrobial compound that inhibit variety of microorganisms. The objectives of the research are to determine the optimum condition and glucose utilization in relation to antimicrobial compound production. Two species of Lactic Acid Bacteria namely Lactococcus and Lactobacillus were used as probiotic. The Lactic Acid Bacteria were fermentated in different medium, initial substrate pH and incubation temperature for the production of antimicrobial compound. The test organisms such as E.coli and Salmonella were selected as test organisms. Amongst the two species of Lactic Acid Bacteria, Lactococcus produced the highest amount of antimicrobial compound than Lactobacillus

Controlled overproduction of proteins by lactic acid bacteria

Lactic acid bacteria are widely used in industrial food fermentations, contributing to flavour, texture and preservation of the fermented products. Here we describe recent advances in the development of controlled gene expression systems, which allow the regulated overproduction of any desirable protein by lactic acid bacteria. Some systems benefit from the fact that the expression vectors, marker genes and inducing factors can be used directly in food applications since they are all derived from food-grade lactic acid bacteria. These systems have also been employed for the development of autolytic bacteria, suitable for various industrial applications.

Characterization of Lactic Acid Bacteria as Probiotic Candidate to Overcome the Salmonellosis in Broiler

Can endanger human health if they contain antibiotic residues in livestock products that can cause resistance to germs in the human body when consuming them. Along with the enactment of a policy to limit the use of antibiotics in animal feed, the use of probiotics as a substitute is urgently needed. This research has been conducted in the Research Center for Veterinary Science inorder to produce probiotic candidates to control the growth of Salmonella sp in chickens that have the potential to pollute the environment and cause foodborne disease. Research on probiotics in chicken has conducted in Indonesian Research Center for Veterinary Science in order to produce probiotic candidates to control the growth of Salmonella sp. in chicken that potentially contaminated the environment causing foodborne disease. The present study aims to evaluate the probiotic potential of indigenous bacteria isolated from cattle in Bogor and select candidates to be used as probiotic. The study was initiated by screening localisolates for probiotic, following by inhibition test against a target of pathogenic bacteria (Salmonella enterica serotype of Typhimurium B0046/ATCC 13311 and serotype of Enteritidis B2893/ATCC 13076), pathogenicity in vitro, survival in thechicken gut, and the lifespan in the lyophilized container. The results showed that six isolates bacteria consisting of Aerococcus viridans B2776, Bifidobacterium dentium B2754 and B2755, Enterococcus faecium B2758, Lactobacillus casei B2752, and Streptococcus uberis B2757 had been selected as the candidate for probiotics. They had specifications namely: anti-microbialsubstance (in vitro) against S. enterica serotype Typhimurium BCC B0046 and serotype Enteritidis BCC B2893, not pathogenic, able to form colonies in intestinal broilers for 40 days with concentrations of >1010 CFU/gram with lifespan up to one year (lyophilized), using protectant serum of inositol 5% or 7,5% skim milk at 5C with concentrations >log10 CFU/ml. The potentialcharacteristics qualified them as probiotics against pathogenic bacteria and could be used to control salmonellosis in the broiler

Isolasi Dan Identifikasi Bakteri Asam Laktat Pada Caecum Ayam Daging (Isolation and Ldentitication of Lactic Acid Bacteria in the Broiler's Caecum)

Lactic acid bacteria is one of natural microbe which is often used as fermentation agent. The Purpose of this researchwas isolated and identified lactic acid bacteria in broiler's caecum. Exploration experimental method by 6 repetition with 2 sub research was used. The ldentification of Lactic acid bacteria was based on morphological, biochemical and physiological of the character. The morphological characteristic were shape cell and gram test. The biochemical characteristic was catalase test. The physiological characteristic was clear zone test. The result obtained l2 isolates of Lactic acid bacteria, rod and coccus , gam positive, catalase negative and showed clear zone colony at lok CaCOt in MRS. That were early characteristic of lactic acid bacteria which were apparenly as genus of Lactobacillus and Leuconostoc

ANTAGONISM ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TRADITIONAL FERMENTED MEAT PETIS

The research of antagonism activity of lactic acid bacteria to pathogen bacteria, spoilage bacteria and histamin producer bacteria was done. The goal of the research was to know the influence of lactic acid bacteria on antagonistic activity to pathogen bacteria, spoilage bacteria, and histamin-producer bacteria. The research used exploration experimental method with 4 replications. This research used lactic acid bacteria which was isolated from traditional fermented meat petis The code of lactic acid bacteria were Pediococcus YDA1 sp., Pediococcus YDA2 sp., Pediococcus YDA3 sp., Pediococcus YDA4 sp., Pediococcus YDA5 sp., Pediococcus YDA6 sp., Pediococcus YDA7 sp., Pediococcus YDA8 sp., Pediococcus YDA9 sp., Pediococcus YDA10 sp., and Pediococcus YDA11 sp. The spoilage bacteria was Bacillus substilis FNCC 0061. The pathogen bacteria were Salmonella typimurium FNCC 0134, Escherchia coli FNCC 0047, Staphylococcus aureus FNCC 0097, and Vibrio parahaemolyticus FNCC 0166. While the codes of the histamin-producer bacteria were P1, P2, and P3. The measurement of antagonistic activity of lactic acid bacteria used well diffusion modification method. This was represent by clear zone diametres as capability of inhibitory. The result of research was that 5 lactic acid bacteria had antagonism activity to pathogen bacteria, spoilage bacteria and histamin-producer bacteria. They were Pediococcus YDA1 sp., Pediococcus YDA3 sp., Pediococcus YDA4 sp., Pediococcus YDA10 sp., and Pediococcus YDA11 sp. Keywords : Pediococcus, Fermented Meat, Antagonism Activit