RNA interference knockdown of BRASSINOSTEROID INSENSITIVE1 in maize reveals novel functions for brassinosteroid signaling in controlling plant architecture

Brassinosteroids (BRs) are plant hormones involved in various growth and developmental processes. The BR signaling system is well established in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) but poorly understood in maize (Zea mays). BRASSINOSTEROID INSENSITIVE1 (BRI1) is a BR receptor, and database searches and additional genomic sequencing identified five maize homologs including duplicate copies of BRI1 itself. RNA interference (RNAi) using the extracellular coding region of a maize zmbril complementary DNA knocked down the expression of all five homologs. Decreased response to exogenously applied brassinolide and altered BR marker gene expression demonstrate that zmbriI-RNAi transgenic lines have compromised BR signaling. zmbriI-RNAi plants showed dwarf stature due to shortened internodes, with upper internodes most strongly affected. Leaves of zmbriI-RNAi plants are dark green, upright, and twisted, with decreased auricle formation. Kinematic analysis showed that decreased cell division and cell elongation both contributed to the shortened leaves. A BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1-yellow fluorescent protein (BES1-YFP) transgenic line was developed that showed BR-inducible BES1-YFP accumulation in the nucleus, which was decreased in zmbriI-RNAi. Expression of the BES1-YFP reporter was strong in the auricle region of developing leaves, suggesting that localized BR signaling is involved in promoting auricle development, consistent with the zmbriI-RNAi phenotype. The blade-sheath boundary disruption, shorter ligule, and disrupted auricle morphology of RNAi lines resemble KNOTTED1-LIKE HOMEOBOX (KNOX) mutants, consistent with a mechanistic connection between KNOX genes and BR signaling

Mesocotyl elongation in Digitaria sanguinalis during seedling development

The mesocotyl is an embryonic organ present in Poaceae that plays an important role in seedling emergence. The elongation of this first internode contributes decisively to the coleoptile reaching the soil surface. This study examines the process of mesocotyl elongation under controlled conditions in three caryopsis collection sites of Digitaria sanguinalis (L.) Scop. originating from Spain (Barcelona and Girona) and Argentina that may have two patterns of germination: radicular or coleoptilar. The frequencies of the two germination patterns varied significantly depending on the origin. Light inhibited the elongation of the mesocotyl drastically, resulting in maximum lengths of 3.5mm, while in darkness the maximum length was 57mm. The time-course evolution displayed under dark conditions was quite similar for all sites of origin and both germination patterns; the growth rate ranged from 0.23 to 0.30mmh21. Within localities, caryopses with a coleoptilar pattern of germination showed a lower growth rate than those with a radicular one.Postprint (author's final draft

Efecto de la defoliación post-quema sobre el crecimiento de dos gramíneas nativas en el Caldenal, Argentina

El crecimiento en los pastizales naturales semiáridos puede ser afectado por la longitud de tiempo que el pastoreo es excluido durante el período de regeneración post-fuego. El objetivo de este trabajo a campo fue estudiar el efecto de la defoliación post-fuego en diferentes estados fenológicos: vegetativo (defoliación temprana) y elongación de entrenudos (defoliación tardía) sobre el crecimiento de Piptochaetium napostaense y Poa ligularis, dos gramíneas nativas perennes muy importantes en la región semiárida templada central de Argentina (Caldenal). En general, los tratamientos de defoliación post-quema no afectaron el número de hojas verdes en las macollas de P. napostaense. La defoliación temprana después del tratamiento de fuego generalmente redujo el número de hojas verdes en las macollas de P. ligularis con respecto a las plantas control. Contrariamente, la defoliación tardía usualmente incrementó el número de hojas verdes en las macollas de P. ligularis. Sin embargo, en ambos casos, muy pocas de las diferencias detectadas fueron significativas (p0,05) a aquellas de las plantas no defoliadas. Inmediatamente después de los tratamientos de defoliación temprana y tardía, las tasas de crecimiento relativas de la altura y la longitud verde total de las macollas de las plantas de ambas especies fueron reducidas (p0.05) than values on non-defoliated plants, respectively. Immediately after early- and late-season defoliation treatments, relative growth rates for tiller height and total green length were reduced (p<0.05) on plants of both species with respect to control plants. Our results suggest that a one-year-period without severe defoliations after fire would be at least necessary for not risking the persistence of these perennial grasses in the Caldenal community.Fil: Pelaez, Daniel Valerio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Boo, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Mayor, M. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Elia, Omar Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Martinez, S. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentin

Straw biomass - potential raw material for ethanol production

Barley straw was found to be suitable raw material for ethanol production. Straw could be easily pre-treated with steam explosion, hydrolysed to monosaccharides and fermented to ethanol. The optimum harvesting times for bio-ethanol raw material are at grain maturity for barley straw. Potential raw material, barley harvested at full ear emergence, when straw is rich in soluble sugars, need further research

Photoreceptor cross-talk in UV-B photomorphogenesis in tomato (Solanum lycopersicum) : screening through phytochrome and cryptochrome mutants : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology at Massey University, Palmerston North, New Zealand

Figures 1.1, 1.3, 1.4 and 1.5 removed due to copyright reasonsPlant photoreceptors detect changes in the light environment and induce differential gene expression, resulting in the appropriate physiological and morphological responses. Under full sunlight, phytochromes, cryptochromes and the UV-B photoreceptor, UVR8 (UV-B RESISTANCE LOCUS 8), destabilize PHYTOCHROME INTERACTING FACTORS (PIFs) to inhibit elongation. PIFs are transcriptions factors that inhibit light-regulated genes, including auxin-related genes involved in cell elongation. In the shaded environment, the reduction in the spectral composition detected by the photoreceptors results in the activation of elongation and PIF activity. However, recent studies have shown that low levels of UV-B can still inhibit the elongation under shade. Most photobiology studies that investigated plant responses to shade have concentrated on the model species, Arabidopsis thaliana. In contrast, Solanum lycopersicum (tomato) is another model system, but few studies have investigated plant responses to shade in tomato due to its sympodial architecture and presence of internodes which A. thaliana lacks. In this study, phytochrome and cryptochrome tomato mutants were exposed to low levels of UV-B under photosynthetically active radiation (PAR) as background light to investigate the possible crosstalk between these photoreceptors and the UV-B photoreceptor of tomato in regulating hypocotyl or internode elongation. Out of all the multiple phytochrome and one cryptochrome mutants, phyAphyB2 mutant exhibited an impaired UV-B inhibition of internode elongation after three days of UV-B treatment. End-point PCR on the gene expression of PIF4 together with two UV-B responsive genes and genes involved in the catabolism of active gibberellin could not explain the impaired response of phyAphyB2. Nevertheless, physiological measurements indicate that phyA and phyB2 of tomato may be acting redundantly in mediating the UV-B induced inhibition of internode

Differential coupling of gibberellin responses by Rht-B1c suppressor alleles and Rht-B1b in wheat highlights a unique role for the DELLA N-terminus in dormancy

During the Green Revolution, substantial increases in wheat (Triticum aestivum) yields were realized, at least in part, through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles. In contrast to Rht-B1b and Rht-D1b, the Rht-B1c allele is characterized by extreme dwarfism and exceptionally strong dormancy. Recently, 35 intragenic Rht-B1c suppressor alleles were created in the spring wheat cultivar Maringa, and termed overgrowth (ovg) alleles. Here, 14 ovg alleles with agronomically relevant plant heights were reproducibly classified into nine tall and five semi-dwarf alleles. These alleles differentially affected grain dormancy, internode elongation rate, and coleoptile and leaf lengths. The stability of these ovg effects was demonstrated for three ovg alleles in different genetic backgrounds and environments. Importantly, two semi-dwarf ovg alleles increased dormancy, which correlated with improved pre-harvest sprouting (PHS) resistance. Since no negative effects on grain yield or quality were observed, these semi-dwarf ovg alleles are valuable for breeding to achieve adequate height reduction and protection of grain quality in regions prone to PHS. Furthermore, this research highlights a unique role for the first 70 amino acids of the DELLA protein, encoded by the Rht-1 genes, in grain dormancy

AXR1 acts after lateral bud formation to inhibit lateral bud growth in Arabidopsis

The AXR1 gene of Arabidopsis is required for many auxin responses. The highly branched shoot phenotype of mature axr1 mutant plants has been taken as genetic evidence for a role of auxin in the control of shoot branching. We compared the development of lateral shoots in wild-type Columbia and axr1-12 plants. In the wild type, the pattern of lateral shoot development depends on the developmental stage of the plant. During prolonged vegetative growth, axillary shoots arise and develop in a basal-apical sequence. After floral transition, axillary shoots arise rapidly along the primary shoot axis and grow out to form lateral inflorescences in an apical-basal sequence. For both patterns, the axr1 mutation does not affect the timing of axillary meristem formation; however, subsequent lateral shoot development proceeds more rapidly in axr1 plants. The outgrowth of lateral inflorescences from excised cauline nodes of wild-type plants is inhibited by apical auxin. axr1-12 nodes are resistant to this inhibition. These results provide evidence for common control of axillary growth in both patterns, and suggest a role for auxin during the late stages of axillary shoot development following the formation of the axillary bud and several axillary leaf primordia

Laboratory Evaluation of Mefluidide Effects on Elongation of Hydrilla and Eurasian Watermilfoil

The potential of mefluidide (N-(2,4-dimethyl-5[[trifluromethyl) sulfonyl] amino] phenol) acetamide) to act as a submersed aquatic plant growth regulator was evaluated using a laboratory bioassay system. Main stem elongation of hydrilla (Hydrilla verticillata (L.f.) Royle) and Eurasian watermilfoil (Myriophyllum spicatum L.) was effectively reduced by mefluidide at low concentrations. The lowest effective concentration of mefluidide that reduced stem length in Eurasian watermilfoil (100 yg a.i./L) was 5 times lower than that for hydrilla (500 yg a.i./L). Short-term net photosynthetic rates of these plants were not affected by mefluidide at concentrations as high as 1000 yg a.i./L. The minimum exposure time required to maintain an inhibitory effect for at least 28 days at a concentration of 500 yg ai.i./L was 3 to 7 days for Eurasian watermilfoil and 7 to 14 days for hydrilla. The results suggest that mefluidide is a more effective growth regulator for Eurasian watermilfoil than hydrilla. Exogenously applied gibberellic acid (GA) did not completely overcome the inhibitory effect of mefluidide even when GA was added at a high concentration (10-5 M). In addition, the internodal lengths of stems treated with mefluidide were not reduced as they were when treated with gibberellin synthesis inhibitors. The reduction of main stem elongation by mefluidide appeared to be due to the inhibition of new cell and tissue development at the stem tip rather than from inhibition of GA biosynthesis