End-use related physical and mechanical properties of selected fast-growing poplar hybrids (Populus trichocarpa x P-deltoides)

This study focused on physical and mechanical properties of fast-growing poplar clones in relation to potential end uses with high added value. A total of 14 trees from three different clones, all P. trichocarpa x deltoides (T x D) hybrids, were felled in a poplar plantation in Lille (Belgium): six 'Beaupre', four 'Hazendans' and four 'Hoogvorst'. Growth rate was found to have no significant influence on the physical mechanical properties. Although the investigated clones are genetically closely related, important variations in physical and mechanical properties were observed. Specific features such as spatial distribution of tension wood and dimensional stability are the main quality factors. It was concluded that 'Beaupre' is suitable for a wide range of high value added applications, such as plywood or construction wood. 'Hazendans' and 'Hoogvorst' will need adapted technology in processing. Further research is needed to characterize clonally induced variation in properties and to assess adequate processing strategies for multiclonal poplar stands

Meta-analysis of a large industrial latex diagnosis database provides insight on Hevea brasiliensis clonal adaptation and site-specific yield potential in Western Africa

A meta-analysis of the Latex Diagnosis (LD) database gathering all LD data stored from 2006 to 2018 in SIFCA/SIPH plantations of Côte d'Ivoire (SAPH), Ghana (GREL) and Nigeria (RENL) has been performed. Average clonal LD data comprising Sucrose (Suc), Inorganic Phosphorus (Pi), Reduced Thiols (RSH) and latex Total Solid Content (TSC) were analyzed and compared for different plantation sites of SAPH (Toupah, Ousrou, Bongo, Rapides Grah, Digahio, Divo and Bettie), GREL (Abura) and RENL (Osse River, New Land, Utagba Uno, Araromi, Waterside and Iguobazuwa). The database was filtered in order to keep only the LD data obtained either from conventional and standard tapping systems (S/2 downward and S/4 upward), excluding the intensified period before slaughtering. Data were processed and analyzed on clones GT1, RRIM600, PR107, AV2037, RRIC100, AF261, PB5/51, PB217, PB235, PB260, PB312, PB314, IRCA18, IRCA41, IRCA109, IRCA111, IRCA130, IRCA209 and IRCA230. All clones were tapped either in d4 or d5 6d/7 tapping frequencies. Stimulation was applied on panel (Pa), under industrial stimulation rates adapted to each clone metabolism and clonal sugar loading characteristics. Based on the relation between Suc and Pi, the analysis of these LD data confirms the latex physiological positioning in clonal typology of all clones. It confirms in particular the high latex sugar loading capacity of clones PB217 (as this clone obtains the highest latex Suc level whatever the plantation site), IRCA109, IRCA41 and IRCA230. It also reveals a systematic positive correlation for all sites between RSH and Suc latex contents: Clones with high latex Suc maintain higher RSH latex concentrations than clones with lower Suc, confirming at industrial scale earlier research results. The physiological hypothesis is that a high clonal latex sugar loading, allowing higher stimulation rates and improved stimulation response, would as well maintain higher latex RSH levels resulting in an improved resistance to oxidative stress resulting from latex metabolic activation. This improved scavenging protection would therefore have positive effects on membranes integrity, on latex stability, on latex flow and on tapping panel dryness (TPD) onset. Moreover, as tapping intensity (tapping frequency and stimulation) was almost similar in all sites, we also make the hypothesis that the latex RSH level and the Pi x RSH value might be accurate indicators to describe, at plantation site level, the local clonal suitability as well as the global stress conditions of trees on each plantation site. This study will be completed later on in order to set up a standard interpretation method of such LD databases

Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge's disease.

In this study, because excessive polycythemia is a predominant trait in some high-altitude dwellers (chronic mountain sickness [CMS] or Monge's disease) but not others living at the same altitude in the Andes, we took advantage of this human experiment of nature and used a combination of induced pluripotent stem cell technology, genomics, and molecular biology in this unique population to understand the molecular basis for hypoxia-induced excessive polycythemia. As compared with sea-level controls and non-CMS subjects who responded to hypoxia by increasing their RBCs modestly or not at all, respectively, CMS cells increased theirs remarkably (up to 60-fold). Although there was a switch from fetal to adult HgbA0 in all populations and a concomitant shift in oxygen binding, we found that CMS cells matured faster and had a higher efficiency and proliferative potential than non-CMS cells. We also established that SENP1 plays a critical role in the differential erythropoietic response of CMS and non-CMS subjects: we can convert the CMS phenotype into that of non-CMS and vice versa by altering SENP1 levels. We also demonstrated that GATA1 is an essential downstream target of SENP1 and that the differential expression and response of GATA1 and Bcl-xL are a key mechanism underlying CMS pathology

Effect of environmental stress on clonal structure of Eucypris virens (Crustacea, Ostracoda)

Environmental stress imposes strong natural selection on clonal populations, promoting evolutionary change in clonal structure. Environmental stress may also lead to reduction in population size, which together with clonal selection may reduce genotypic diversity of the local populations. We examined how clonal structure in wild-collected samples of two parthenogenetic populations of the freshwater ostracod Eucypris virens responded to hypersalinity and starvation, and the combination of the two stressors. We applied the stress treatments in a factorial design for one generation. When 60% of the individuals per experimental unit had died, post-experimental clonal structure was compared to that of the start of the experiment, which reflected the field conditions. We used five polymorphic allozyme loci as genotype markers. All stress treatments reduced survival compared to the control treatment. In the population "Rivalazzetto”, we observed a reduction of clonal richness in the control treatment, with the initially dominant clone maintaining dominance. This may have resulted from interclonal competition and clone-specific survival under the different laboratory conditions. Clonal richness remained high in the salinity treatment while it was reduced in the combined stress and starvation treatments. In the population "Fornovo”, clonal richness reduced in all treatments including control, while the salinity and combined stress treatment reduced clonal evenness. The clone dominating at the start of the experiment increased in frequency in all treatments, but the change in clonal structure during the experiment was more pronounced in this population. These results suggest that in some conditions an intermediate level of environmental stress may lessen the decline in genetic diversity by strong inter-clonal competition. Moreover, the variation in clonal structure among the stress treatments and distinct genetic backgrounds indicates that more general predictions of stress effects on clonal structure may be difficul

Impaired ATP synthase assembly associated with a mutation in the human ATP synthase subunit 6 gene

Mutations in human mitochondrial DNA are a well recognized cause of disease. A mutation at nucleotide position 8993 of human mitochondrial DNA, located within the gene for ATP synthase subunit 6, is associated with the neurological muscle weakness, ataxia, and retinitis pigmentosa (NARP) syndrome. To enable analysis of this mutation in control nuclear backgrounds, two different cell lines were transformed with mitochondria carrying NARP mutant mitochondrial DNA. Transformant cell lines had decreased ATP synthesis capacity, and many also had abnormally high levels of two ATP synthase sub-complexes, one of which was F1-ATPase. A combination of metabolic labeling and immunoblotting experiments indicated that assembly of ATP synthase was slowed and that the assembled holoenzyme was unstable in cells carrying NARP mutant mitochondrial DNA compared with control cells. These findings indicate that altered assembly and stability of ATP synthase are underlying molecular defects associated with the NARP mutation in subunit 6 of ATP synthase, yet intrinsic enzyme activity is also compromised

Senescence in vitro and ionising radiations—the human diploid fibroblast model

The influence of ionising radiations on ageing is still controversial. Since Hayflick established the concept that diploid cells have finite lifespan in vitro, human diploid fibroblast (HDF) cultures have been recognised as a potent experimental model for cytogerontological investigations. In this study HDF cultures in phase II were exposed to acute irradiation with either X-rays on fast neutrons. The replicative potentials and labelling indices with [3H]thymidine were measured post irradiation until the cultures ceased growth in phase III. Cell mortality was measured by cloning. The apparent loss in replicative potential of irradiated mass cultures was wholly attributable to the loss of viable clonogenic cells. The current concept of precocious clonal senescence in vitro as a late effect of irradiation in clonogenic survivors is not supported by the present experiments. Instead, our results suggest that exposure to a single dose of ionising radiations either causes total replicative incapacitation (killing) of HDF cells and their progeny early after irradiation or leaves their replicative potentials unperturbed

Aging-dependent functional alterations of mitochondrial DNA (mtDNA) from human fibroblasts transferred into mtDNA-less cells

To investigate the role that aging-dependent accumulation of mitochondrial DNA (mtDNA) mutations plays in the senescence processes, mitochondria from fibroblasts of 21 normal human individuals between 20 weeks (fetal) and 103 years of age were introduced into human mtDNA-less (ρ0) 206 cells by cytoplast × ρ0 cell fusion, and 7-31 transformant clones were isolated from each fusion. A slight cell donor age-dependent decrease in growth rate was detected in the transformants. Using an O2 consumption rate of 1 fmol/min/cell, which was not observed in any transformant among 158 derived from individuals 20 weeks (fetal) to 37 years of age, as a cut-off to identify respiratory-deficient clones, 11 such clones were found among 198 transformants derived from individuals 39-103 years of age. Furthermore, conventional and nonparametric analysis of the respiratory rates of 356 clones revealed a very significant decrease with donor age. In other analyses, a very significant age-dependent decline in the mtDNA content of the clones was observed, without, however, any significant correlation with the decrease in O2 consumption rate in the defective transformants. These observations clearly indicate the occurrence in the fibroblast-derived transformants of two independent, age-related functional alterations of mtDNA, presumably resulting from structural damage to this genome

Polyploid races, genetic structure and morphological features of earthworm Aporrectodea rosea (Savigny, 1826) (Oligohaeta: Lumbricidae) in Ukraine

Four chromosomal races (2n=36, 3n=54, 6n=108, 8n=144) and 96 clones have been revealed among 224 specimens of the earthworm A. rosea over the territory of Ukraine by means of karyological analysis and biochemical genetic marking. Each population has been showed by several clones at least; moreover the clones from different places have never been identical. The clones in the range of one population can be identified with the set of quantitative and qualitative parameters