Intratumoral chemotherapy in an integumentary squamous cell carcinoma in a cockatiel (Nymphicus hollandicus)

An eight-year-old, female cockatiel (Nymphicus hollandicus) was presented with anorexia, lethargy, a mass at the lower side of the wing and discoloration of the feathers. Physical examination showed an ulcerated nodular integumentary lesion of approximately 4 cm(3) ventromedial on the wing at the side of the propatagium and the humerus. Lateral and ventrodorsal radiographs revealed only hepatomegaly. After a stabilization period, surgical excision of the tumor was performed. Based on histopathological evaluation and bacterial culture of the surgically removed tissue, the lesion was typed as an integumentary squamous cell carcinoma with secondary bacterial infection (Corynebacterium sp). Four weeks postoperative, the tumor had recurred. Chemotherapeutic treatment was started with intratumoral carboplatin (1.5 mg/cm(3)) once a week. Because of further tumor growth after the second administration of carboplatin, resection of the mass was performed before the next infiltration. However, the bird died during anesthesia. Despite increase in tumor size, histopathological and immunohistochemical evaluations showed degeneration of the tumor with intercellular edema and vacuolization of the tumor cells, presumably resulting from carboplatin administration. More research is needed to investigate the efficacy and safety of the intratumoral administration of carboplatin as a treatment option in birds with integumentary squamous cell carcinoma

Health Effects Associated With Electronic Cigarette Use: Automated Mining of Online Forums.

BACKGROUND:Our previous infodemiological study was performed by manually mining health-effect data associated with electronic cigarettes (ECs) from online forums. Manual mining is time consuming and limits the number of posts that can be retrieved. OBJECTIVE:Our goal in this study was to automatically extract and analyze a large number (>41,000) of online forum posts related to the health effects associated with EC use between 2008 and 2015. METHODS:Data were annotated with medical concepts from the Unified Medical Language System using a modified version of the MetaMap tool. Of over 1.4 million posts, 41,216 were used to analyze symptoms (undiagnosed conditions) and disorders (physician-diagnosed terminology) associated with EC use. For each post, sentiment (positive, negative, and neutral) was also assigned. RESULTS:Symptom and disorder data were categorized into 12 organ systems or anatomical regions. Most posts on symptoms and disorders contained negative sentiment, and affected systems were similar across all years. Health effects were reported most often in the neurological, mouth and throat, and respiratory systems. The most frequently reported symptoms and disorders were headache (n=939), coughing (n=852), malaise (n=468), asthma (n=916), dehydration (n=803), and pharyngitis (n=565). In addition, users often reported linked symptoms (eg, coughing and headache). CONCLUSIONS:Online forums are a valuable repository of data that can be used to identify positive and negative health effects associated with EC use. By automating extraction of online information, we obtained more data than in our prior study, identified new symptoms and disorders associated with EC use, determined which systems are most frequently adversely affected, identified specific symptoms and disorders most commonly reported, and tracked health effects over 7 years

Genome-wide screening of mouse knockouts reveals novel genes required for normal integumentary and oculocutaneous structure and function.

Oculocutaneous syndromes are often due to mutations in single genes. In some cases, mouse models for these diseases exist in spontaneously occurring mutations, or in mice resulting from forward mutatagenesis screens. Here we present novel genes that may be causative for oculocutaneous disease in humans, discovered as part of a genome-wide screen of knockout-mice in a targeted single-gene deletion project. The International Mouse Phenotyping Consortium (IMPC) database (data release 10.0) was interrogated for all mouse strains with integument abnormalities, which were then cross-referenced individually to identify knockouts with concomitant ocular abnormalities attributed to the same targeted gene deletion. The search yielded 307 knockout strains from unique genes with integument abnormalities, 226 of which have not been previously associated with oculocutaneous conditions. Of the 307 knockout strains with integument abnormalities, 52 were determined to have ocular changes attributed to the targeted deletion, 35 of which represent novel oculocutaneous genes. Some examples of various integument abnormalities are shown, as well as two examples of knockout strains with oculocutaneous phenotypes. Each of the novel genes provided here are potentially relevant to the pathophysiology of human integumentary, or oculocutaneous conditions, such as albinism, phakomatoses, or other multi-system syndromes. The novel genes reported here may implicate molecular pathways relevant to these human diseases and may contribute to the discovery of novel therapeutic targets

Thermal Burns and Smoke Inhalation Injuries

In this pathophysiology paper, the reader is presented with a profile of an elderly patient who recently suffered thermal burns and smoke inhalation injuries as a result of a nursing home fire. This patient’s severe burns were classified as deep partial-thickness and full-thickness and her total body surface area (TBSA) of burns was over 15%. This paper details the different types of burns, the varying clinical manifestations of thermal burns, smoke inhalation injuries, laboratory values associated with burns, and the multitude of treatment necessary for each stage of burn management. Wound healing is described as well as potential risks and complications associated with burns. Suggestions for nursing care are also given in order to properly care for a patient similar to the one in this profile

Associations between adjustment disorder and hospital-based infections in the Danish population.

OBJECTIVE:There is some evidence that posttraumatic stress disorder (PTSD) is associated with increased risk of infections, and it is unknown whether adjustment disorder is as well. We assessed the association between adjustment disorder and subsequent infections, and assessed additive interaction with sex. METHODS:The study population included a nationwide cohort of all Danish-born residents of Denmark diagnosed with adjustment disorder between 1995 and 2011, and an age- and sex-matched general population comparison cohort. We compared rates of infections requiring inpatient or outpatient hospitalization in the two cohorts. We fit Cox proportional hazards models to compute adjusted hazard ratios (aHR) for the associations between adjustment disorder and 32 types of infections, and calculated interaction contrasts to assess interaction between adjustment disorder and sex. RESULTS:Adjustment disorder was associated with increased rates of infections overall (n = 19,838 infections, aHR = 1.8, 95% confidence interval = 1.8. 1.9), and increased rates of each individual infection type (aHRs for 30 infections ranged from 1.5 to 2.3), adjusting for baseline psychiatric and somatic comorbidities and marital status. For many infection types (e.g., skin infections, pneumonia), interaction contrasts indicated rate differences were greater among men than women, while for two (urinary tract infections and sexually transmitted infections), rate differences were greater for women. CONCLUSIONS:These findings are consistent with studies examining the relationship between psychological stress and infections, and between PTSD and infections. They may be explained by a combination of the triggering of unhealthy behaviors as well as immune responses to stress

A phylogeny of the tinamous (Aves: Palaeognathiformes) based on integumentary characters

A cladistic analysis of the tinamous, including the 47 currently recognized species and some distinct subspecies, was conducted based on 80 integumentary characters from adult and natal plumage, ramphoteca (corneum sheath of bill), and podoteca (horny scales of legs). For the adult plumage (50 characters), we studied feather pigmentation patterns from different pterylae (feather tracts). A criterion of overlap of basic pigmentation elements was used to assign costs to the transformation between the states in most of these characters in such a way that transformations between more similar conditions were less costly. The consensus tree was almost fully resolved, and about 50% of its groups were relatively well supported. Because the only outgroup that could be used provided a poor root, two possible rootings of the ingroup subtree were considered; in both cases, only one of the two traditional subfamilies (the steppe tinamous) was recovered, and the other (the forest tinamous) appeared as paraphyletic. The results of the present analysis are compared with those from an osteological data set, using a strict supertree technique. The combined tree has a large number of nodes, indicating a high degree of congruence between the two data sets.Fil: Bertelli, Sara Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Giannini, Norberto Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Goloboff, Pablo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Superior de Entomología; Argentin

Suppression of cell-spreading and phagocytic activity on nano-pillared surface: in vitro experiment using hemocytes of the colonial ascidian Botryllus schlosseri.

Nano-scale nipple array on the body surface has been described from various invertebrates including endoparasitic and mesoparasitic copepods, but the functions of the nipple array is not well understood. Using the hydrophilized nanopillar sheets made of polystyrene as a mimetic material of the nipple arrays on the parasites\u2019 body surface, we assayed the cell spreading and phagocytosis of the hemocytes of the colonial ascidian Botryllus schlosseri. On the pillared surface, the number of spreading amebocytes and the number of phagocytizing hemocytes per unit area were always smaller than those on the flat surface (Mann-Whitney test, p < 0.05 - 0.001), probably because the effective area for the cell attachment on the pillared surface is much smaller than the area on the flat sheet. The present results supports the idea that the nipple array on the parasites' body surface reduces the innate immune reaction from the host hemocytes

Disposition and Success of Patients Following Discharge in the Acute Setting

Abstract: Background & Purpose: Many patients are seen in the hospital by physical therapists who also help decide where the patient will go after discharge (e.g., home, rehab hospital, skilled nursing facility) with the goal being the safest and best quality of life. Therapists may use different tests and measures along with their own professional judgment to help make a recommendation for a discharge location. The purpose of this study was to examine how PT’s recommendation for discharge location, POMA score, “6-clicks” score, age, diagnosis, and gender determine patient’s fall/readmission status after being discharged by hospital. Subjects: There were 113 patients in this study. The inclusion criteria for the patients were that they had to have been seen by a physical therapist in the acute care hospital, were given a physical therapist discharge recommendation, and were over the age of 18. Methods: Data was collected from a single community hospital in the Pacific Northwest. The following data were collected: reason for patient admission to the hospital, POMA score, “6-clicks” score, the therapist’s discharge recommendation, patient age, patient gender, and the actual discharge location of the patient. Approximately 30 days following discharge, the patients were contacted via telephone to determine where they went after discharge, if they were readmitted to the hospital within 30 days, or if they experienced a fall since leaving the hospital. Data were analyzed using independent t-tests, chi-square analysis, and receiver operating characteristic (ROC) curves. Results: There were no significant differences between patients’ “6-clicks” scores (p=0.667), POMA scores (p=0.890), or age (p=0.940) when comparing those who experienced a post-discharge fall and those that did not have a fall. No differences in “6-clicks” scores (p=0.815), POMA scores (p=0.753), and age (p=0.735) were found between patients’ who were readmitted and those not readmitted within 30 days of discharge. No associations were found with mismatch-when the actual discharge location was not the same as the physical therapist’s recommendation for discharge (fall p=0.090, readmission p=0.087), medical diagnosis (fall p=0.989, readmission p=0.002*) or gender (fall p=0.737, readmission p=0.250) with patients’ outcomes (reported falls or no falls and readmitted or not readmitted after 30 days from discharge). Areas under the ROC curves with “6-clicks” for fall status (patients who had either fallen or not fallen post discharge 30 days) was 0.463 (95% confidence interval (CI)=0.288, 0.637) and for readmission status (patients who had either readmitted or did not 30 days after discharge) was 0.477 (95% CI=0.351, 0.604). Areas under the curves with POMA scores for fall status was 0.505 (95% CI=0.331, 0.678) and for readmission status was 0.497 (95% CI=0.376, 0.617). Discussion: The POMA, “6-clicks,” and the physical therapist recommendations were not associated with patient falls or readmissions. Because other studies have shown a relationship between “6-clicks” and discharge location, there is a need for more studies that examine this relationship. Future studies should include a larger patient population, from multiple locations to diversify the participants and facilities

NASA Ames Institutional Scientific Collection (ISC)

NASA's current human space flight research is directed towards enabling human space exploration beyond Low Earth Orbit (LEO). The Space Flight Payload Projects; Rodent Research, Cell Science, and Microbial Labs, flown on the International Space Station (ISS), benefit both the global life sciences and commercial space communities. Verified data sets, science results, peer-reviewed publications, and returned biospecimens, collected and analyzed for flight and ground investigations, are all part of the knowledge base within NASAs Human Exploration and Operations Mission Directorates Space Life and Physical Sciences Research and Applications (SLPSRA) Division, specifically the Human Research and Space Biology Programs. These data and biospecimens are made available through the public LSDA website. The Ames Institutional Scientific Collection (ISC), or ARC Biobank, stores flight and ground biospecimens from Space Shuttle and ISS programs. These specimens are curated and managed by the Ames Life Sciences Data Archive (ALSDA), an internal node of NASA's Life Sciences Data Archive (LSDA). The ARC Biolbank stores over 15,000 specimens from experiments dating from 1984 to present. Currently available specimens include tissues from the circulatory, digestive, endocrine, excretory, integumentary, muscular, neurosensory, reproductive, respiratory and skeletal systems. The most recent contributions include RNA, DNA and protein extracts from Rodent Research 1 and tissues from Rodent Research 4. NASA's biospecimen collection represents a unique and limited resource. The use of these biospecimens maximizes utilization and scientific return from these unique spaceflight payload and ground control research subjects. These biospecimens are harvested following complex, costly NASA research activities to meet primary scientific objectives. Once the primary scientific objectives have been met, the remaining specimens are made available to provide secondary opportunities for complementary studies or new investigations to broaden research without large expenditures of time or resources. Innovative ways of sharing this information ultimately advances the frontiers of human space exploration as well as scientific understanding of the effects of gravity on life on earth