Comprehensive compendium of Arabidopsis RNA-seq data, A

2020 Spring.Includes bibliographical references.In the last fifteen years, the amount of publicly available genomic sequencing data has doubled every few months. Analyzing large collections of RNA-seq datasets can provide insights that are not available when analyzing data from single experiments. There are barriers towards such analyses: combining processed data is challenging because varying methods for processing data make it difficult to compare data across studies; combining data in raw form is challenging because of the resources needed to process the data. Multiple RNA-seq compendiums, which are curated sets of RNA-seq data that have been pre-processed in a uniform fashion, exist; however, there is no such resource in plants. We created a comprehensive compendium for Arabidopsis thaliana using a pipeline based on Snakemake. We downloaded over 80 Arabidopsis studies from the Sequence Read Archive. Through a strict set of criteria, we chose 35 studies containing a total of 700 biological replicates, with a focus on the response of different Arabidopsis tissues to a variety of stresses. In order to make the studies comparable, we hand-curated the metadata, pre-processed and analyzed each sample using our pipeline. We performed exploratory analysis on the samples in our compendium for quality control, and to identify biologically distinct subgroups, using PCA and t-SNE. We discuss the differences between these two methods and show that the data separates primarily by tissue type, and to a lesser extent, by the type of stress. We identified treatment conditions for each study and generated three lists: differentially expressed genes, differentially expressed introns, and genes that were differentially expressed under multiple conditions. We then visually analyzed these groups, looking for overarching patterns within the data, finding around a thousand genes that participate in stress response across tissues and stresses

Cross-Platform Text Mining and Natural Language Processing Interoperability - Proceedings of the LREC2016 conference

No abstract available

Cross-Platform Text Mining and Natural Language Processing Interoperability - Proceedings of the LREC2016 conference

No abstract available

Information Integration for Machine Actionable Data Management Plans

Data management plans are free-form text documents describing the data used and produced in scientific experiments. The complexity of data-driven experiments requires precise descriptions of tools and datasets used in computations to enable their reproducibility and reuse. Data management plans fall short of these requirements. In this paper, we propose machine-actionable data management plans that cover the same themes as standard data management plans, but particular sections are filled with information obtained from existing tools. We present mapping of tools from the domains of digital preservation, reproducible research, open science, and data repositories to data management plan sections. Thus, we identify the requirements for a good solution and identify its limitations. We also propose a machine-actionable data model that enables information integration. The model uses ontologies and is based on existing standards

On the Challenges of Implementing Machine Learning Systems in Industry

RÉSUMÉ : Dans l’optique de ce mémoire, nous nous concentrons sur les défis de l’implantation de sys-tèmes d’apprentissage automatique dans le contexte de l’industrie. Notre travail est réparti sur deux volets: dans un premier temps, nous explorons des considérations fondamentales sur le processus d’ingénierie de systèmes d’apprentissage automatique et dans un second temps, nous explorons l’aspect pratique de l’ingénierie de tels systèmes dans un cadre industriel. Pour le premier volet, nous explorons un des défis récemment mis en évidence par la com-munauté scientifique: la reproducibilité. Nous expliquons les défis qui s’y rattachent et, à la lueur de cette nouvelle compréhension, nous explorons un des effets rattachés, omniprésent dans l’ingénierie logicielle: la présence de défaut logiciels. À l’aide d’une méthodologie rigoureuse nous cherchons à savoir si la présence de défauts logiciels, parmis un échantillon de taille fixe, dans un cadriciel d’apprentissage automatique impacte le résultat d’un processus d’apprentissage.----------ABSTRACT : Software engineering projects face a number of challenges, ranging from managing their life-cycle to ensuring proper testing methodologies, dealing with defects, building, deploying, among others. As machine learning is becoming more prominent, introducing machine learn-ing in new environments requires skills and considerations from software engineering, machine learning and computer engineering, while also sharing their challenges from these disciplines. As democratization of machine learning has increased by the presence of open-source projects led by both academia and industry, industry practitioners and researchers share one thing in common: the tools they use. In machine learning, tools are represented by libraries and frameworks used as software for the various steps necessary in a machine learning project. In this work, we investigate the challenges in implementing machine learning systems in the industry

The Federal Big Data Research and Development Strategic Plan

This document was developed through the contributions of the NITRD Big Data SSG members and staff. A special thanks and appreciation to the core team of editors, writers, and reviewers: Lida Beninson (NSF), Quincy Brown (NSF), Elizabeth Burrows (NSF), Dana Hunter (NSF), Craig Jolley (USAID), Meredith Lee (DHS), Nishal Mohan (NSF), Chloe Poston (NSF), Renata Rawlings-Goss (NSF), Carly Robinson (DOE Science), Alejandro Suarez (NSF), Martin Wiener (NSF), and Fen Zhao (NSF). A national Big Data1 innovation ecosystem is essential to enabling knowledge discovery from and confident action informed by the vast resource of new and diverse datasets that are rapidly becoming available in nearly every aspect of life. Big Data has the potential to radically improve the lives of all Americans. It is now possible to combine disparate, dynamic, and distributed datasets and enable everything from predicting the future behavior of complex systems to precise medical treatments, smart energy usage, and focused educational curricula. Government agency research and public-private partnerships, together with the education and training of future data scientists, will enable applications that directly benefit society and the economy of the Nation. To derive the greatest benefits from the many, rich sources of Big Data, the Administration announced a “Big Data Research and Development Initiative” on March 29, 2012.2 Dr. John P. Holdren, Assistant to the President for Science and Technology and Director of the Office of Science and Technology Policy, stated that the initiative “promises to transform our ability to use Big Data for scientific discovery, environmental and biomedical research, education, and national security.” The Federal Big Data Research and Development Strategic Plan (Plan) builds upon the promise and excitement of the myriad applications enabled by Big Data with the objective of guiding Federal agencies as they develop and expand their individual mission-driven programs and investments related to Big Data. The Plan is based on inputs from a series of Federal agency and public activities, and a shared vision: We envision a Big Data innovation ecosystem in which the ability to analyze, extract information from, and make decisions and discoveries based upon large, diverse, and real-time datasets enables new capabilities for Federal agencies and the Nation at large; accelerates the process of scientific discovery and innovation; leads to new fields of research and new areas of inquiry that would otherwise be impossible; educates the next generation of 21st century scientists and engineers; and promotes new economic growth. The Plan is built around seven strategies that represent key areas of importance for Big Data research and development (R&D). Priorities listed within each strategy highlight the intended outcomes that can be addressed by the missions and research funding of NITRD agencies. These include advancing human understanding in all branches of science, medicine, and security; ensuring the Nation’s continued leadership in research and development; and enhancing the Nation’s ability to address pressing societal and environmental issues facing the Nation and the world through research and development