Bloodlines: mammals, leeches, and conservation in southern Asia

Southern Asia is a biodiversity hotspot both for terrestrial mammals and for leeches. Many small-mammal groups are under-studied in this region, while other mammals are of known conservation concern. In addition to standard methods for surveying mammals, it has recently been demonstrated that residual bloodmeals within leeches can be sequenced to find mammals in a given area. While these invertebrate-parasite-derived DNA (iDNA) methods are promising, most of the leech species utilized for this type of survey remain unevaluated, notwithstanding that their diversity varies substantially. Here we examine approximately 750 individual leech specimens in the genus Haemadipsa across a large range in southern Asia (Bangladesh, Cambodia, and China), specifically reviewing the diversity of mammals they feed on and their own genetic structuring. Leeches were found to feed on a considerable variety of mammals, corroborating prior studies. Additionally, leeches were found to have fed both on bats and on birds, neither of which has previously been recorded with this method. The genetic structuring of the leeches themselves revealed 15 distinct clades of which only two precisely corresponded to previously characterized species, indicating that much work is needed to finalize classifications in this genus. Most importantly, with regards to mammal conservation, leeches in these clades appear to feed on a broad range of mammals

iDNA from terrestrial haematophagous leeches as a wildlife surveying and monitoring tool - prospects, pitfalls and avenues to be developed

Invertebrate-derived DNA (iDNA) from terrestrial haematophagous leeches has recently been proposed as a powerful non-invasive tool with which to detect vertebrate species and thus to survey their populations. However, to date little attention has been given to whether and how this, or indeed any other iDNA-derived data, can be combined with state-of-the-art analytical tools to estimate wildlife abundances, population dynamics and distributions. In this review, we discuss the challenges that face the application of existing analytical methods such as site-occupancy and spatial capture-recapture (SCR) models to terrestrial leech iDNA, in particular, possible violations of key assumptions arising from factors intrinsic to invertebrate parasite biology. Specifically, we review the advantages and disadvantages of terrestrial leeches as a source of iDNA and summarize the utility of leeches for presence, occupancy, and spatial capture-recapture models. The main source of uncertainty that attends species detections derived from leech gut contents is attributable to uncertainty about the spatio-temporal sampling frame, since leeches retain host-blood for months and can move after feeding. Subsequently, we briefly address how the analytical challenges associated with leeches may apply to other sources of iDNA. Our review highlights that despite the considerable potential of leech (and indeed any) iDNA as a new survey tool, further pilot studies are needed to assess how analytical methods can overcome or not the potential biases and assumption violations of the new field of iDNA. Specifically we argue that studies to compare iDNA sampling with standard survey methods such as camera trapping, and those to improve our knowledge on leech (and other invertebrate parasite) physiology, taxonomy, and ecology will be of immense future value

A new species of Tritetrabdella (Hirudinida: Hirudiniformes: Haemadipsidae) from northern Indochina

A new species of the terrestrial haemadipsid genus Tritetrabdella, Tritetrabdella longiducta, from northern Indochina is described. The new species is distinguished from all congeners by the combination of triannulate somite VII, uniannulate somite XXV, three lobes of respiratory auricles in somites XXV–XXVII, 57 friction rays on caudal sucker, male gonopore (in somite XI b5/b6) and female gonopore (in somite XII b5) positions and slightly folded vaginal sac. Phylogenetic analyses using nuclear 18S rRNA and 28S rRNA, in addition to mitochondrial cytochrome c oxidase subunit I markers, confirmed that the new species forms a unique lineage among the known congeners. A key to all the species of Tritetrabdella is provided

Poly-paraphyly of Hirudinidae: many lineages of medicinal leeches

<p>Abstract</p> <p>Background</p> <p>Medicinal leeches became infamous for their utility in bloodletting popularized in the 19^thcentury, and have seen a recent resurgence in post-operative treatments for flap and replantation surgeries, and in terms of characterization of salivary anticoagulants. Notorious throughout the world, the quintessential leech family Hirudinidae has been taken for granted to be monophyletic, as has the non-bloodfeeding family Haemopidae.</p> <p>Results</p> <p>This study is the first to evaluate molecular evidence from hirudinid and haemopid leeches in a manner that encompasses the global scope of their taxonomic distributions. We evaluated the presumed monophyly of the Hirudinidae and assessed previous well-accepted classification schemes. The Hirudinidae were found not to be monophyletic, falling instead into two distinct and unrelated clades. Members of the non-bloodfeeding family Haemopidae were scattered throughout the tree and among traditional hirudinid genera. A combination of nuclear 18S rDNA and 28S rDNA with mitochondrial 12S rDNA and cytochrome <it>c </it>oxidase I were analyzed with Parsimony and with Bayesian methods.</p> <p>Conclusion</p> <p>The family Hirudinidae must be refined to include only the clade containing <it>Hirudo medicinalis </it>(European medicinal leech) and related leeches irrespective of bloodfeeding behavior. A second clade containing <it>Macrobdella decora </it>(North American medicinal leech) and its relatives may yet be recognized in Semiscolecidae in order to avoid paraphyly. The African distribution of species from each of the divergent hirudinid clades suggests that a deep divergence took place in the history of the medicinal leeches hundreds of millions of years ago.</p

Feeding strategies and competition between terrestrial Haemadipsa leeches (Euhirudinea : Arhynchobdellida) in Danum Valley rainforest (Borneo, Sabah)

Haemadipsid leeches are among the most successful terrestrial invertebrates in Bornean rainforests. They are very common ectoparasites of vertebrates, and their abundance has facilitated the conduction of numerous projects in the fields of ecology, zoogeography and taxonomy. We undertook research on two species inhabiting lowland dipterocarp forest, Haemadipsa picta Moore, 1929 and Haemadipsa subagilis (Moore, 1929), in order to address the following questions: (a) is there a difference in leech abundance between trails and off-trails?; (b) is ambush location dependent on specimen size or is species-specific?; (c) is intra- and interspecific competition limited by differences in foraging behaviours or vertical niche partitioning? Our results clearly show that H. picta is more abundant on trails than on off-trails and is vertically dispersed within the understory; the size of a specimen is strongly correlated with plant height. Haemadipsa subagilis was found not to exhibit such patterns. We suggest a possible lowering of interspecific competition between these species as a result of: (i) size-dependent dispersion of H. picta (together with reduction of intraspecific competition); and (ii) habitat specialisation of H. subagilis. Moreover, we provide new observations on their foraging behaviour

국내 미기록인 흡혈성 산거머리 Haemadipsa rjukjuana Oka, 1910 보고

The terrestrial leeches from the peripheral island of the Korean Peninsula were identified as Haemadipsa rjukjuanaOka, 1910. The arhynchobdellid family Haemadipsidae and H. rjukjuana are newly added into the Korean leechfauna. This species is blood-feeding leech that attacks birds and medium or large sized mammals primarily,including human. The sequence of mitochondrial cytochrome c subunit I (COI), and the additional biology for thisspecies are presented. This is the first study of terrestrial blood-feeding leeches in Korea.OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000051105/10SEQ:10PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000051105ADJUST_YN:NEMP_ID:A077262DEPT_CD:551FILENAME:haemadipsa rjukjuana (131204).pdfDEPT_NM:수의학과SCOPUS_YN:NCONFIRM:

Ideating IDNA: Lessons and Limitations From Leeches in Legacy Collections

Indirect methods for conducting faunal inventories present great promise, and genomic inventories derived from environmental sources (eDNA) are improving. Invertebrate ingested DNA (iDNA) from terrestrial leeches in the family Haemadipsidae has shown potential for surveying vertebrates and biodiversity monitoring in protected areas. Here we present an initial, and critical, evaluation of the limitations and biases of current iDNA protocols for biodiversity monitoring using both standard and NGS barcoding approaches. Key findings include the need for taxon relevant multi-locus markers and reference databases. In particular, the limitations of available reference databases have profound potential to mislead and bias eDNA and iDNA results if not critically interpreted. Nevertheless, there is great potential for recovery of amplifiable DNA from gut contents of invertebrate museum specimens which may reveal both temporal patterns and cryptic diversity in protected areas with increased efficiency. Our analyses of ingested DNA (iDNA) from both freshly stored and previously collected (legacy) samples of terrestrial leeches successfully identified vertebrates from Myanmar, Australia and Madagascar and indicate the potential to characterize microbial communities, pathogen diversity and interactions at low cost

Diversity, distribution, and abundance of annelida in UiTM Negeri Sembilan forest reserve / Siti Khadijah Baniran and Nursyazni Abdul Rahim

Annelida is a universal, abundant, and varied collection of creatures that can be discovered on terrestrial, freshwater as well as in marine. Their biological importance relies not only on the comparatively high number of species but also on their often-high abundance. However, knowledge of Annelida in UiTM Negeri Sembilan Forest Reserve is poorly known and documented resulted due to a lack of awareness of their role in the ecosystem. Hence, this research was done to identify the species and investigate the diversity and abundance of Annelida in three different areas in UiTM Negeri Sembilan Forest Reserve. This research was advantageous in helping to manage, preserve, protect, and conserve Annelida’s habitat for ecological sustainability and may contribute to society's knowledge and information to other researchers about Annelida’s diversity. A sampling collection was conducted seven times in April 2022 at Site A, Site B and Site C. The specimens were hand-sorted and placed in a plastic pail. Then, the specimens were brought to the laboratory for the identification process. A total of 134 individual Annelida samples were collected from Site A (N = 85), Site B (N = 11) and Site C (N = 38). From detailed identifications and observations based on morphological characteristics, they were classified into two species which are Pontoscolex corethrurus (N = 110) and Haemadipsa zeylanica (N = 24). The statistical analysis shows the richness (R) = 0.20, diversity (H) = 0.47, evenness (E) = 0.68. The Analysis of Variance (ANOVA) of this study shows both length and weight were significant to distinguish between P. corethrurus and H. zeylanica species. Based on this research, the Annelida diversity in the UiTM Forest Reserve is relatively low. However, further detailed research is needed to discover more about the variety of annelids in this area