Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain

Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No. 2012J06008 and 201311180002) and the projects-sponsored by SRF. TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.Peer reviewedPostprin

Aquaculture Asia, Vol. 11, No. 2, pp.1-38. April-June 2006

Selection of high-health postlarvae: a prerequisite for sustainability of the Indian shrimp industry, by S. Saurabh, V. Kumar, S. Karanth and G. Venkateshwarlu. Nucleotides: novel immunostimulants in aquaculture feed, by P. Kalaimani Arasi, N. Felix. Brood stock and all female scampi grow out ponds in south India, by P. Balamurugan, C. Balasundaram, P. Mariappan and S. Dharaneedharan. Genetically modified fish and its potential applications, by M. Karthik, D.B. Akolkar, V. Kumar, and S.D. Singh. Rainbow trout farming in hill terrace of Nuwakot, Nepal, by Tek Bahadur Gurung, Sadhu Ram Basnet and Krishna Prasad Lamsal. Sugar industry by-products as plankton boosters and yield enhancers in carp culture systems, by S. Felix, T. Francis, K. Mohan Naidu, C. Senthil and P. Herald Robins. A note on the growth and production of Penaeus monodon in low saline culture systems of Kerala, by G. Prasad. Rural aquaculture: poor households raise prawns for export, by Peter Edwards. Marine fish marketing systems in coastal Bangladesh: potential for development, by Nesar Ahmed and Helen T. Sturrock

Environmental stress upon hepatopancreatic cells of freshwater prawns (Decapoda: Caridea) from the floodplain of Paraná River

In order to evaluate the influence of stressed environments on hepatopancreatic cells of freshwater prawns, Macrobachium borellii Nobili, 1896 and Palaemonetes argentinus Nobili 1901, (Crustacea, Decapoda, Palaemonidae) were collected at three different aquatic environments with different relationship to urban development in Argentina. Furthermore the effects of several cypermethryn concentration on hepatopancreatic cell of M. borellii and P. argentinus were evaluated in a laboratory assays. The "N° 1" lake (Santa Fe Argentina) which is more affected by the anthropogenic pressure and "Don Felipe" lake which is still not strongly by human activities were the studied sites from the floodplain of Paraná river. While Alejandra lake was the intermedia effects sites. Different damaged ultrastructures were found in F- and R-cells of prawns in the stressed lake. The predominant features were: disrupted the microvillous border, swelled mitochondria, reduction of endoplasmic reticulum, dyctiosomes, glycoproteins, desnaturalization of vacuole membrane and premature autolysis. Moreover the F-cell number was higher in the environment near to city than in the others sites. Similar effects were observed in the cypermethryn assays. The observations clearly indicate that the ultrastructure of midgut gland in the both palaemonids varies depending on the site from which animals are collected and the biocid presence. So, in this case it can be stated that the hepatopancreas histology of freshwater prawns is a good tool to monitor the impact of a stressed environment upon freshwater prawns.Fil: Collins, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentin

Simultaneous observations of haemolymph flow and ventilation in marine spider crabs at different temperatures: a flow weighted MRI study

In vivo magnetic resonance imaging (MRI) and angiography were applied to the marine spider crab Maja squinado for a study of temperature effects and thermal tolerance. Ventilation and haemolymph circulation were investigated during progressive cooling from 12°C to 2°C. The anatomical resolution of MR images from Maja squinado obtained with a standard spin echo sequence were suitable to resolve the structures of various internal organs. The heart of the animal could be depicted without movement artifacts. The use of a flow compensated gradient echo sequence allowed simultaneous observations of ventilation, reflected by water flow through the gill chambers as well as of haemolymph flow. Simultaneous investigation of various arteries was possible by use of flow weighted MRI. In addition to those accessible by standard invasive flow sensitive doppler sensors, flow changes in gill, leg arteries and the venous return could be observed. Both ventilation and haemolymph flow decreased during progressive cooling and changes in haemolymph flow varied between arteries. Haemolymph flow through the Arteria sternalis, some gill and leg arteries was maintained at low temperatures indicating a reduced thermal sensitivity of flow in selected vessels. In support of previous invasive studies of haemolymph flow as well as heart and ventilation rates, the results demonstrate that the operation of gills and the maintenance of locomotor activity are critical for cold tolerance. A shift in haemolymph flow between arteries likely occurs to ensure the functioning of locomotion and ventilation in the cold

Bioaccumulation of Microcystins by Freshwater Mussels in Mystic Lake and Middle Pond, MA

The UNH Center for Freshwater Biology investigated a possible relationship between a cyanobacteria bloom and a large-scale die-off of freshwater mussels in Mystic Lake and Middle Pond (Barnstable, MA). Four mussel species, Elliptio complanata (Eastern Elliptio), Pyganodon cataracta (Eastern Floater), Leptodea ochracea (Tidewater Mucket), and Lampsilis radiata (Eastern Lampmussel) (Nedeau, 2008), along with water samples, were collected from these lakes on August 9, 2010 (during bloom) and again on September 29 and October 8, 2010 (post-bloom). Hepatopancreas tissue, foot tissue, and water samples were tested for the cyanobacteria toxins, microcystins (MC), using ELISA techniques. MC concentrations in the hepatopancreas were generally higher (171.2 ng MC g-1 dry weight (dw)) than in the muscle (foot) tissue (55.8 ng MC g -1 dw) for each species. Average microcystin concentrations in mussels sampled during postbloom tissues were slightly lower (161.6 ng MC g-1 dw) than those collected during the cyanobacteria bloom (171.2 ng MC g1 dw). Live mussels were also subjected to a depuration experiment to determine the release of MC from mussels into the water. Mussels that were placed in cyanobacteria-free water depurated 61-90% MC within the first few days demonstrating their ability to release free MC-cyanotoxins into the lake water

Aquaculture Asia, vol. 8, no. 4, pp.1-52, October - December 2003

CONTENTS: Freshwater prawn, Macrobrachium nobilii a promising candidate for rural nutrition, by Pitchimuthu Mariappan, P. Balamurugan, and Chellam Balasundaram. Snapshots of a clean, innovative, socially responsible fishfarm in Sri Lanka, by Pedro Bueno. Introduction of rainbow trout Onchorynchus mykiss in Nepal: Constraints and prospects, by Tek Bahadur Gurung, Sadhu Ram Basnet. Tilapia seed production in Ho Chi Minh City, Southern Vietnam, by H. P. V. Huy, A. MacNiven, N. V. Tu, Ram C. Bhujel and David C. Little. Seaweed Mariculture: Scope And Potential In India, by Sajid I. Khan and S. B. Satam. Growth enhancement of carp and prawn through dietary sodium chloride supplementation, by P.Keshavanath, B. Gangadhara and Savitha Khadri. Fertilization, soil and water quality management in small-scale ponds, by S. Adhikari Shrimp harvesting technology on the south west coast of Bangladesh, by S. M. Nazmul Alam, Michael J. Phillips and C. K. Lin. The “Gher Revolution”, by M.C. Nandeesha [Farmers as Scientists series] Aquaculture Compendium – case study component, by Peter Edwards. Rice-Fish Culture in China, by Fang Xiuzhen. Exercising responsibilities to tackle aquatic animal diseases, by CV Mohan. Application of immunostimulants in larviculture: Feasibility and challenges, by ZhouJin. Marine Finfish Sectio

Per os infectivity of white spot syndrome virus (WSSV) in white-legged shrimp (Litopenaeus vannamei) and role of peritrophic membrane

As earlier observations on peroral infectivity of WSSV in white-legged shrimp are conflicting, here, a standardized peroral intubation technique was used to examine (i) the role of the physical composition of the viral inoculum and (ii) the barrier function of the PM. In a first experiment, the infectivity of a WSSV stock was compared by determining the SID50 by intramuscular injection, peroral inoculation or via feeding. The following titers were obtained: 108.77 SID50/g by intramuscular injection, 10(1.23) SID50/g by peroral inoculation and 100.73 SID50/g by feeding. These results demonstrated that 10(7.54)-10(8.03) infectious virus is needed to infect shrimp by peroral inoculation and via feeding. Next, it was examined if damage of the PM may increase the susceptibility for WSSV by peroral route. The infectivity of a virus stock was tested upon peroral inoculation of shrimp with and without removal of the PM and compared with the infectivity upon intramuscular inoculation. The virus titers obtained upon intramuscular injection and peroral inoculation of shrimp with and without PM were 10(8.63), 10(1.13) and 10(1.53) SID50/mL, respectively. This experiment confirmed the need of 10(7.1)-10(7.5) infectious virus to infect shrimp via peroral route and showed that the removal of the PM slightly but not significantly (p > 0.05) facilitated the infection of shrimp. This study indicated that WSSV contaminated feed is poorly infectious via peroral route, whereas it is highly infectious when injected into shrimp. The PM plays a minor role as internal barrier of shrimp against WSSV infection

Laboratory study of the impact of repetitive electrical and mechanical stimulation on brown shrimp Crangon crangon

Pulse trawling is currently the best available alternative to beam trawling in the brown shrimp Crangon crangon and Sole Solea solea (also known as Solea vulgaris) fisheries. To evaluate the effect of repetitive exposure to electrical fields, brown shrimp were exposed to the commercial electrodes and pulse settings used to catch brown shrimp (shrimp startle pulse) or Sole (Sole cramp pulse) 20 times in 4 d and monitored for up to 14 d after the first exposure. Survival, egg loss, molting, and the degree of intranuclear bacilliform virus (IBV) infection were evaluated and compared with those in stressed but not electrically exposed (procedural control) and nonstressed, nonexposed (control) brown shrimp as well as brown shrimp exposed to mechanical stimuli. The lowest survival at 14 d (57.3%) occurred in the Sole cramp pulse treatment, and this was significantly lower than in the group with the highest survival, the procedural control (70.3%). No effect of electrical stimulation on the severity of IBV infection was found. The lowest percentage of molts occurred in the repetitive mechanical stimulation treatment (14.0%), and this was significantly lower than in the group with the highest percentage of molts, the procedural control (21.7%). Additionally, the mechanically stimulated brown shrimp that died during the experiment had a significantly larger size than the surviving individuals. Finally, no effect of the shrimp startle pulse was found. Therefore, it can be concluded that repetitive exposure to a cramp stimulus and mechanical stimulation may have negative effects on the growth and/or survival of brown shrimp. However, there is no evidence that electrical stimulation during electrotrawls would have a larger negative impact on brown shrimp stocks than mechanical stimulation during conventional beam trawling