Offspring subcutaneous adipose markers are sensitive to the timing of maternal gestational weight gain

peer-reviewedBackground Excessive maternal weight gain during pregnancy impacts on offspring health. This study focused on the timing of maternal gestational weight gain, using a porcine model with mothers of normal pre-pregnancy weight. Methods Trial design ensured the trajectory of maternal gestational weight gain differed across treatments in early, mid and late gestation. Diet composition did not differ. On day 25 gestation, sows were assigned to one of five treatments: Control sows received a standard gestation diet of 2.3 kg/day (30 MJ DE/day) from early to late gestation (day 25–110 gestation). E sows received 4.6 kg food/day in early gestation (day 25–50 gestation). M sows doubled their food intake in mid gestation (day 50–80 gestation). EM sows doubled their food intake during both early and mid gestation (day 25–80 gestation). L sows consumed 3.5 kg food/day in late gestation (day 80–110 gestation). Offspring body weight and food intake levels were measured from birth to adolescence. Markers of lipid metabolism, hypertrophy and inflammation were investigated in subcutaneous adipose tissue of adolescent offspring. Results The trajectory of gestational weight gain differed across treatments. However total gestational weight gain did not differ except for EM sows who were the heaviest and fattest mothers at parturition. Offspring birth weight did not differ across treatments. Subcutaneous adipose tissue from EM offspring differed significantly from controls, with elevated mRNA levels of lipogenic (CD36, ACACB and LPL), nutrient transporters (FABP4 and GLUT4), lipolysis (HSL and ATGL), adipocyte size (MEST) and inflammation (PAI-1) indicators. The subcutaneous adipose depot from L offspring exhibited elevated levels of CD36, ACACB, LPL, GLUT4 and FABP4 mRNA transcripts compared to control offspring. Conclusions Increasing gestational weight gain in early gestation had the greatest impact on offspring postnatal growth rate. Increasing maternal food allowance in late gestation appeared to shift the offspring adipocyte focus towards accumulation of fat. Mothers who gained the most weight during gestation (EM mothers) gave birth to offspring whose subcutaneous adipose tissue, at adolescence, appeared hyperactive compared to controls. This study concluded that mothers, who gained more than the recommended weight gain in mid and late gestation, put their offspring adipose tissue at risk of dysfunction.This research was funded by Teagasc, under the National Development Plan. LBMcN was in receipt of a Teagasc Walsh Fellowship. Nestle hosted LG on a sabbatical and funded the RT-PCR cost

GESTATION LENGTH OF KARI SHEEP

Previously, the concept of a gestation period of sheep shorter than 121 days was unheard of in the field of sheep biology. Our findings during a farmers’ survey in the Lotkho area of Chitral, Pakistan, revealed that the sheep native to the region, locally called “Kari”, gestates for a period of only three months. This duration is 25-40% short of the usual gestation length and is shorter than ever recorded for the species. The mean and mode gestation length (GL) was 110 and 92 days respectively and was concentrated in three distinct clusters: day 87-95, 120-123 and 151-153, accounting for 52% of all observations. GL was influenced by location, season of conception and lambing and the interaction of location with season; had an estimated repeatability of zero. The mean lambing interval was 224.7 ±5.24 days, ranging between 109 and 467 days. During a trial conducted in a controlled environment, only three out of the 27 copulated ewes conceived, and gestated in 113, 125 and 145 days respectively. Gestation length during the trial supported field findings. The results suggest that GL in Kari sheep is unusual as many ewes gestated in three months, with variations of up to 5 months. Genotype by environment interaction is a possibility. However, reasons for the findings are still not clear and further controlled studies should be carried out to establish and further explore the factor(s) responsible for this unusual and unique manifestation of the Kari with regards to its gestation length

Effects of antenatal betamethasone on preterm human and mouse ductus arteriosus: comparison with baboon data.

BackgroundAlthough studies involving preterm infants ≤34 weeks gestation report a decreased incidence of patent ductus arteriosus after antenatal betamethasone, studies involving younger gestation infants report conflicting results.MethodsWe used preterm baboons, mice, and humans (≤276/7 weeks gestation) to examine betamethasone's effects on ductus gene expression and constriction both in vitro and in vivo.ResultsIn mice, betamethasone increased the sensitivity of the premature ductus to the contractile effects of oxygen without altering the effects of other contractile or vasodilatory stimuli. Betamethasone's effects on oxygen sensitivity could be eliminated by inhibiting endogenous prostaglandin/nitric oxide signaling. In mice and baboons, betamethasone increased the expression of several developmentally regulated genes that mediate oxygen-induced constriction (K+ channels) and inhibit vasodilator signaling (phosphodiesterases). In human infants, betamethasone increased the rate of ductus constriction at all gestational ages. However, in infants born ≤256/7 weeks gestation, betamethasone's contractile effects were only apparent when prostaglandin signaling was inhibited, whereas at 26-27 weeks gestation, betamethasone's contractile effects were apparent even in the absence of prostaglandin inhibitors.ConclusionsWe speculate that betamethasone's contractile effects may be mediated through genes that are developmentally regulated. This could explain why betamethasone's effects vary according to the infant's developmental age at birth

Normal pregnancy is associated with an increase in thrombin generation from the very early stages of the first trimester

Background: Pregnancy is a hypercoagulable state associated with an increased risk of venous thrombosis, which begins during the first trimester, but the exact time of onset is unknown. Thrombin generation, a laboratory marker of thrombosis risk, increases during normal pregnancy but it is unclear exactly how early this increase occurs. Methods: We assessed thrombin generation by Calibrated Automated Thrombography in women undergoing natural cycle in vitro fertilization, who subsequently gave birth at term following a normal pregnancy (n = 22). Blood samples were taken just prior to conception and repeated five times during very early pregnancy, up to Day 59 estimated gestation. Results: Mean Endogenous Thrombin Potential (ETP), peak thrombin generation and Velocity Index (VI) increased significantly from pre-pregnancy to Day 43 gestation (p = 0.024–0.0004). This change persisted to Day 59 gestation. The mean of the percentage change from baseline, accounting for inter-individual variation, in ETP, peak thrombin and VI increased significantly from pre-pregnancy to Day 32 gestation (p = 0.0351–<0.0001) with the mean increase from baseline persisting to Day 59 gestation. Conclusion: Thrombin generation increases significantly during the very early stages of normal pregnancy when compared to the pre-pregnancy state. The increased risk of venous thrombosis therefore likely begins very early in a woman's pregnancy, suggesting that women considered clinically to be at high thrombotic risk should start thromboprophylaxis as early as possible after a positive pregnancy test

Consumer Preferences for Animal Welfare Attributes: The Case of Gestation Crates

Animal welfare concerns are having dramatic impacts on food and livestock markets. Here we examine consumer preferences for pork products with a focus on use of gestation crates. We examine underlying consumer valuations of pork attributes while considering preference heterogeneity as well as voluntary and legislative alternatives in producing gestation crate-free pork. Our results suggest that prohibiting swine producers from using gestation crates fails to improve consumer welfare in the presence of a labeling scheme documenting voluntary disadoption of gestation crates. Consumers are found to implicitly associate animal welfare attributes with smaller farms. Preference heterogeneity drives notably diverse consumer welfare impacts when pork produced with use of gestation crates is no longer available for consumption.animal welfare, consumer welfare, economics of legislation, gestation crates, pork, swine, voluntary labeling, willingness to pay, Agribusiness, Agricultural and Food Policy, Consumer/Household Economics, Industrial Organization, Livestock Production/Industries, Q11, Q13, Q18,

Pre-eclampsia toxaemia

A 33 year old primagravida rhesus positive woman was referred to the Emergency Department by her family doctor at 27 weeks gestation. At 25 weeks of gestation, she had been diagnosed with hypertension and was started on labetalol therapy. At 27 weeks of gestation she was admitted in view of persistently raised blood pressure which was not being controlled with Labetalol, generalised (facial, hands and lower limbs) oedema and frequent frontal headaches. The patient also complained of photophobia. Following examination it was found that the patient was suffering from pre-eclampsia toxaemia. Delivery was expedited in view of the developing complications. Pre-eclampsia toxaemia is a multi-system disorder which manifests as hypertension and proteinuria after 20 weeks of gestation. The disease originates from the placenta and is cured by delivery. There is blood vessel endothelial damage with a maternal inflammatory response which leads to vasospasm, increased capillary permeability and clotting dysfunction which account for hypertension, proteinuria, reduced placental blood flow and reduced cerebral perfusion resulting in eclampsia.peer-reviewe

Effects of gestational age at birth on cognitive performance : a function of cognitive workload demands

Objective: Cognitive deficits have been inconsistently described for late or moderately preterm children but are consistently found in very preterm children. This study investigates the association between cognitive workload demands of tasks and cognitive performance in relation to gestational age at birth. Methods: Data were collected as part of a prospective geographically defined whole-population study of neonatal at-risk children in Southern Bavaria. At 8;5 years, n = 1326 children (gestation range: 23–41 weeks) were assessed with the K-ABC and a Mathematics Test. Results: Cognitive scores of preterm children decreased as cognitive workload demands of tasks increased. The relationship between gestation and task workload was curvilinear and more pronounced the higher the cognitive workload: GA2 (quadratic term) on low cognitive workload: R2 = .02, p<0.001; moderate cognitive workload: R2 = .09, p<0.001; and high cognitive workload tasks: R2 = .14, p<0.001. Specifically, disproportionally lower scores were found for very (<32 weeks gestation) and moderately (32–33 weeks gestation) preterm children the higher the cognitive workload of the tasks. Early biological factors such as gestation and neonatal complications explained more of the variance in high (12.5%) compared with moderate (8.1%) and low cognitive workload tasks (1.7%). Conclusions: The cognitive workload model may help to explain variations of findings on the relationship of gestational age with cognitive performance in the literature. The findings have implications for routine cognitive follow-up, educational intervention, and basic research into neuro-plasticity and brain reorganization after preterm birth

Comparison of transcutaneous ultrasound over the right flank with transrectal ultrasonography in the diagnosis of pregnancy in New Zealand dairy herds : a thesis presented in partial fulfillment of the requirements for the degree of Master of Veterinary Science at Massey University

Application of a 3.5 MHz sector transducer over the right flank allows the rapid and clear visualization of bovine pregnancy (ie: fetus, fetal membranes, fetal fluid and/or placentomes). A total of 1736 cows in ten commercial, pasture-based New Zealand dairy herds were examined for pregnancy by transcutaneous ultrasound across the right flank and transrectal ultrasound between 37 and 198 days of gestation. The gold standard was derived from calving records or examination at slaughter. The overall sensitivity of transrectal ultrasound (96.24%) was markedly higher than flank ultrasound (58.55%) and the overall probability of a correct diagnosis of pregnancy status was also significantly higher (p<0.0001). From 155 days of gestation, however, flank ultrasound represented a more accurate method of pregnancy diagnosis and the probability of a correct diagnosis was significantly higher (p<0.0001) after this gestational age. The gestational age of 225 cows from four Spring-calving dairy herds was determined and ultrasound pregnancy test recorded, to determine possible fetal characteristics able to be visualized via transcutaneous ultrasound over the right flank in order to age pregnancy during mid to late gestation. Linear or quadratic equations and curves were formulated from 60 to 198 days of gestation. The fetal characteristics of thoracic diameter, abdominal diameter or umbilical diameter can be used to age pregnancy from 60 days of gestation. Placentome height and length were not significant in the determination of gestational age