Speech Communication

Contains research objectives and summary of research on six research projects and reports on three research projects.National Institutes of Health (Grant 5 RO1 NS04332-13)National Institutes of Health (Fellowship 1 F22 MH5825-01)National Institutes of Health (Grant 1 T32 NS07040-01)National Institutes of Health (Fellowship 1 F22 NS007960)National Institutes of Health (Fellowship 1 F22 HD019120)National Institutes of Health (Fellowship 1 F22 HD01919-01)U. S. Army (Contract DAAB03-75-C-0489)National Institutes of Health (Grant 5 RO1 NS04332-12

Extracellular proteinases in natural isolates of Staphylococci

U ovom radu su detaljnije biohemijski okarakterisane proteinaze prirodnih izolata Staphylococcus sp. F22, F86, M104, S2007 i S2105. Utvrđeno je da se radi o proteinazama relativno male molekulske mase (od 20 do 32 kDa) koje spadaju u prave ekstracelularne enzime, pošto se sa površine ćelije odvajaju u medijum za rast. Temperaturni optimumi ovih proteinaza se kreću od 30 do 37°C, a pH optimumi u opsegu od 6,5 do 8. Joni bakra inhibiraju njihovu aktivnost, dok joni kalcijuma stimulišu aktivnost proteinaza iz izolata F22 M104 i S2007. Pored kazeinskih frakcija, proteinaze stafilokoka hidrolizuju i druge proteinske supstrate, kao što su želatin i BSA. U eksperimentima sa proteinaznim inhibitorima utvrđeno je da proteinaze izolata F22 i M104 pripadaju serinskoj klasi, proteinaze S2007 i S2105 klasi metaloproteinaza dok proteinazu izolata F86 na ovaj način nije bilo moguće precizno klasifikovati.Biochemical characteristics of proteinases from natural isolates of Staphylococcus sp. F22, F86, M104, S2007 and S2105 have been studied. It was found that these proteinases have relatively low molecular masses (from 20 to 32 kDa), and that they are released from the cell envelope in to the growth medium. Their temperature optima are between 30 and 37°C and their pH optima range from 6,5 to 8. Copper ions inhibit their activity, but the presence of calcium ions stimulates the activity of proteinases from isolates F22, M104 and S2007. Beside casein fractions, they also hydrolyze heterologous protein substrates, such as BSA and gelatin. Experiments with specific proteinase inhibitors revealed that proteinases from isolates F22 and M104 belong to the serine group of proteinases, S2007 and S2105 proteinases were classified as metalloproteinases. Type of F86 proteinase in these experiments could not be clearly determinated

Application of sludge-based carbonaceous materials in a hybrid water treatment process based on adsorption and catalytic wet air oxidation

This paper describes a preliminary evaluation of the performance of carbonaceous materials prepared from sewage sludges (SBCMs) in a hybrid water treatment process based on adsorption and catalytic wet air oxidation; phenol was used as the model pollutant. Three different sewage sludges were treated by either carbonisation or steam activation, and the physico-chemical properties of the resultant carbonaceous materials (e.g. hardness, BET surface area, ash and elemental content, surface chemistry) were evaluated and compared with a commercial reference activated carbon (PICA F22). The adsorption capacity for phenol of the SBCMs was greater than suggested by their BET surface area, but less than F22; a steam activated, dewatered raw sludge (SA_DRAW) had the greatest adsorption capacity of the SBCMs in the investigated range of concentrations (<0.05 mol L−1). In batch oxidation tests, the SBCMs demonstrated catalytic behaviour arising from their substrate adsorptivity and metal content. Recycling of SA_DRAW in successive oxidations led to significant structural attrition and a hardened SA_DRAW was evaluated, but found to be unsatisfactory during the oxidation step. In a combined adsorption–oxidation sequence, both the PICA carbon and a selected SBCM showed deterioration in phenol adsorption after oxidative regeneration, but a steady state performance was reached after 2 or 3 cycles

Speech Communication

Contains research objectives and summary of research on four research projects.National Institutes of Health (Grant 5 RO1 NS04332-14)National Institutes of Health (Grant 5 T32 NS07040-02)National Institutes of Health (Fellowship 1 F22 NS00796-01)National Institutes of Health (Grant 1 ROI NS13028-01)National Institutes of Health (Grant 5 T3Z NS07040-02)National Institutes of Health (Fellowship 1 F22 MH58258-02)U. S. Army- Maryland Procurement Office (Contract MDA904-76-C-0331

The reactions \gwl\ and \gzl\ in SU(N)SU(N) strongly interacting theories

Building on recent phenomenology of \gpi, we discuss the expectation for two photon production of longitudinal gauge boson pairs in $SU(N)$ technicolor theories. The treatment involves a matching of dispersive techniques with the methodology of chiral perturbation theory.Comment: 25 LaTeX pages (incl. 10 figures on 7 pages, also in plain LaTeX, also available from the authors in PostScript form via regular E_mail), UMHEP-39