19,598 research outputs found

    Two-Stream Action Recognition-Oriented Video Super-Resolution

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    We study the video super-resolution (SR) problem for facilitating video analytics tasks, e.g. action recognition, instead of for visual quality. The popular action recognition methods based on convolutional networks, exemplified by two-stream networks, are not directly applicable on video of low spatial resolution. This can be remedied by performing video SR prior to recognition, which motivates us to improve the SR procedure for recognition accuracy. Tailored for two-stream action recognition networks, we propose two video SR methods for the spatial and temporal streams respectively. On the one hand, we observe that regions with action are more important to recognition, and we propose an optical-flow guided weighted mean-squared-error loss for our spatial-oriented SR (SoSR) network to emphasize the reconstruction of moving objects. On the other hand, we observe that existing video SR methods incur temporal discontinuity between frames, which also worsens the recognition accuracy, and we propose a siamese network for our temporal-oriented SR (ToSR) training that emphasizes the temporal continuity between consecutive frames. We perform experiments using two state-of-the-art action recognition networks and two well-known datasets--UCF101 and HMDB51. Results demonstrate the effectiveness of our proposed SoSR and ToSR in improving recognition accuracy.Comment: Accepted to ICCV 2019. Code: https://github.com/AlanZhang1995/TwoStreamS

    Complementarity of PALM and SOFI for super-resolution live cell imaging of focal adhesions

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    Live cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenging task for super-resolution microscopy. We have addressed this important issue by combining photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI). Using simulations and fixed cell focal adhesion images, we investigated the complementarity between PALM and SOFI in terms of spatial and temporal resolution. This PALM-SOFI framework was used to image focal adhesions in living cells, while obtaining a temporal resolution below 10 s. We visualized the dynamics of focal adhesions, and revealed local mean velocities around 190 nm per minute. The complementarity of PALM and SOFI was assessed in detail with a methodology that integrates a quantitative resolution and signal-to-noise metric. This PALM and SOFI concept provides an enlarged quantitative imaging framework, allowing unprecedented functional exploration of focal adhesions through the estimation of molecular parameters such as the fluorophore density and the photo-activation and photo-switching rates
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