Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates

Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format

Constraints Imposed by Rift Inheritance on the Compressional Reactivation of a Hyperextended Margin: Mapping Rift Domains in the North Iberian Margin and in the Cantabrian Mountains

This work has been financed by the Ministry of Science and Innovation of Spain through the Projects MARCAS: CTM2009-11522 and MISTERIOS: MINECO13-CGL2013-48601-C2-2-R and the Government of Asturias and the FEDER funds through the Project GEOCANTABRICA: GRUPIN14-044. P. Cadenas held a Severo Ochoa PhD grant from the Education Council of Asturias. This grant funded two short-term stays of P. Cadenas in the National Oceanography Centre of Southampton and in the University of Strasbourg/EOST of Strasbourg. J. Tugend and G. Manatschal are supported by the MM4 consortium. T.A. Minshull is supported by a Wolfson Research Merit award. The CS01 2-D seismic data and the boreholes used in this study were purchased through the ATH (http://geoportal.minetur.gob.es/ATHvz/). The interpretation of the 2-D time migrated stacks and the boreholes was carried out using Kingdom and Petrel E&P software, which were provided by IHS and Schlumberger, respectively, as part of educational programmes. Maps were produced with ArcGIS-ESRI software

An overview of genus Aesculus L.: ethnobotany, phytochemistry, and pharmacological Activities

The genus Aesculus L. (Hippocastanaceae) has 12 species distributed in eastern Asia, eastern and western North America, and Europe. In Europe and the United States, A. hippocastanum has been used for the treatment of chronic venous insufficiency, hemorrhoids, and postoperative edema. In China, A. chinensis var. chinense has been used as a stomachic and analgesic in the treatment of distention and pain in the chest and the abdomen, malaria, dysentery, and heart disease. The objective of this paper is to review the ethnobotany, phytochemistry, and pharmacological properties of the genus Aesculus. To date, more than 210 compounds have been isolated and identified primarily from the fruits or seeds of Aesculus, with polyhydroxylated triterpenoid saponins as the major active principles. Studies have confirmed that Aesculus seed extracts and isolates possess diverse activities, including anti-inflammatory, antitumor, antiviral, antifungal, antiangiogenic (vascular protection), anti-obesity, antioxidative, and antigenotoxic properties. Interestingly, detailed studies of the saponins from more eastern North American species and other uninvestigated Aesculus species are necessary to further confirm the chemotaxonomic value of the novel cytotoxic saponins discovered recently from A. pavia, an eastern North American species, and to fully understand the chemical profile of the genus. Animal investigations of these novel cytotoxic saponins on the potential for the treatment of cancer are also intriguin

La biblioteca cervantina de Carolina Michaëlis de Vasconcelos en la Universidade de Coimbra

La biblioteca de Carolina Michäelis de Vasconcelos (1851-1925), depositada en la Universidade de Coimbra, de la que fue profesora desde 1911 –como la primera mujer que impartió clase en dicha universidad– presenta numerosos ejemplares de obras cervantinas, algunos de los cuales se expusieron al público a raíz del IV Centenario del Quijote de 2005. Destaca el hecho de que la mayor parte de las obras de Cervantes que conservaba la erudita procedían de editoriales alemanas, de ciudades como Leipzig y Gotha, algunas de las más antiguas de los primeros años del siglo XIX, escritas en español o alemán. Guarda, además, dos Quijotes del siglo XIX, escritos en español, pero editados en París y Leipzig. Mi propósito es dar a conocer el legado cervantino de la filóloga luso-alemana, así como su relación con cervantistas españoles y los intereses cervantinos que demostraba, a través de su biblioteca personal

Department of Metabolic Engineering

薬物代謝工学部門は和漢薬の薬効，毒性発現に関与する代謝系の分子生物学的研究を発展させることを設置目的とし，①和漢薬の薬効発現に関与する腸内細菌遺伝子の解析，②薬物代謝機能調節遺伝子の解明とその応用，③腎毒性物質産生機構の分子生物学的解明とその制御に関する研究を課題として取りあげ，和漢薬の薬効発現機構，生体へのレスポンスなどの基礎的研究を通じて，和漢薬の科学的評価や臨床応用をはかることを目指している。主な研究題目を以下に示す。1. 天然物のバイオトランスフォーメイション2. 和漢薬の薬効発現に関与する腸内細菌遺伝子の解明3. AIDSの予防および治療薬の開発4. 腎疾患における病態の解明と腎臓病治療薬の開発この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されまし

Paleogeographic inversion resulting from large out of sequence breaching thrusts: The León Fault (Cantabrian Zone, NW Iberia). A new picture of the external Variscan Thrust Belt in the Ibero-Armorican Arc

Out of sequence breaching thrusts may give rise to duplication of a former thrust stack in map view in the same manner that stratigraphic units are repeated by initial thrusts. In this way, large breaching thrusts may put an inicial paleogeographic pattern out of order, producing apparent paleogeographic inversions at regional scale. The León Fault, an orogen-scale fault located in the Variscan foreland fold-thrust belt of the Iberian Peninsula, known as the Cantabrian Zone, has caused much controversy in the understanding of Iberian Paleozoic tectonics. The León Fault displays all the features of a breaching thrust and from this interpretation a simple paleogeographic pattern results, once both breaching and former thrusts are restored, in contrasts with previously reported paleogeographic models. This approach implies a redefinition of the geological provinces of the Cantabrian Zone, since some major nappes of that zone, so far considered as different tectonic units, are reinterpreted as one single unit repeated by the León Thrust. It also has implications in discriminating between the various kinematic models proposed for the larger Ibero-Armorican Arc, favouring a late bending of an initial linear belt instead of a progressive change in transport direction during nappe emplacement. Finally, the paleogeographic inversion caused by the León breaching Thrust is compared to other orogen-scale paleogeographic inversions associated with antiformal stacks, such as the preAlps with respect to the Helvetic Nappes, and the Ligurian-Tuscan domains in the Apennines