EFFECT OF COENZYME Q10 ALONE AND ITS COMBINATION WITH ROSUVASTATIN ON STREPTOZOTOCIN-NICOTINAMIDE INDUCED DIABETIC NEUROPATHY IN RATS

Objectives: This study was aimed to investigate the effect of coenzyme Q10 and its combination with rosuvastatin on STZ-nicotinamide induced diabetic neuropathy.Methods: Diabetic neuropathy in rats were induced with streptozotocin-nicotinamide. The diabetic rats were treated with coenzyme Q10 or rosuvastatin or their combination. Various parameters like muscular grip strength, paw withdrawal response, tail flick response and markers of oxidative stress such as malondialdehyde (MDA) level, superoxide dismutase (SOD) and reduced glutathione (GSH) in the sciatic nerve were measured. All treated animal was subjected to histopathological changes of sciatica nerve.Results: In diabetic control group, muscular grip strength was significantly decreased and increased paw withdrawal response, tail flick response as compared to normal control rats. In addition, STZ-nicotinamide caused nerve cell damage with a higher MDA level, depletion of SOD and GSH level along with marked degeneration of the nerve cell. The treatment of diabetic rats with coenzyme Q10 or rosuvastatin or their combination ameliorate STZ-nicotinamide induced nerve damage due to improvement in the muscular grip strength, paw withdrawal response, tail flick response, reduction in oxidative stress along with histopathological changes.Conclusion: This finding suggests that treatment with coenzyme Q10 or rosuvastatin showed significant neuroprotective effect against STZ-nicotinamide induced diabetic neuropathy. However, concomitant administration of both showed a better neuroprotective effect than coenzyme Q10 or rosuvastatin alone treatment.Â

Effects of Camellia sinensis Leaves Extract on Some Biochemical Parameters in Alloxan-Induced Diabetic Rats

This study was designed to evaluate the effect of aqueous leaves extract Camellia sinensis on serum glucose levels and liver enzyme markers in alloxan-induced diabetic rats. Diabetes was induced by a single dose of intraperitoneal injection of 150mg/kg bwt of alloxan, and 100, 200 and 400 mg/kg bwt of the extract was administered orally to different groups of diabetic rats for 3 weeks. A significant decrease (p<0.05) in serum glucose, Total bilirubin (TB) and the liver marker enzymes (Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and alkaline phosphatase (ALP)) was observed in groups 4 and 5. The hypoglycemic effects as well as its effects on liver enzymes makes it an attractive recipe for the prophylactic treatment of diabetes. Keywords: Camellia sinensis, diabetes, liver marker enzymes, rats

Acupuncture Mechanism and Redox Equilibrium

Oxidative stress participates in the pathological process of various diseases. Acupuncture is a component of the health care system in China that can be traced back for at least 3000 years. Recently, increased evidences indicate that acupuncture stimulation could reduce oxidative damage in organisms under pathological state, but the exact mechanism remains unclear. This review focuses on the emerging links between acupuncture and redox modulation in various disorders, such as vascular dementia, Parkinson’s disease, and hypertension, ranging from redox system, antioxidant system, anti-inflammatory system, and nervous system to signaling pathway. Although the molecular and cellular pathways studies of acupuncture effect on oxidative stress are preliminary, they represent an important step forward in the research of acupuncture antioxidative effect

A study of hypoglycemic effect of Caesalpinia bonduc extract on alloxan induced diabetic albino rats

Background: The objective of the study was to scientifically investigate the oral hypoglycemic activity of Caesalpinia bonduc on Alloxan induced diabetic albino rats. To compare the hypoglycemic effect of Caesalpinia bonduc with that of the standard drug Glibenclamide used in the treatment of diabetes mellitus.Methods: Adult healthy albino rats of wister strain of either sex weighing 150-200gms were included in the study. The animals were divided into 4 groups namely control, diabetic control, standard and test groups with 6 animals in each group. Diabetes was chemically induced using alloxan to produce hyperglycemia in rats. Standard drug Glibenclamide suspended in gum acacia was administered for standard group. Test drug Caesalpinia bonduc was administered for test group. Morning around 9 a.m. blood glucose levels were recorded on 1st, 3rd, 7th, 14th, 21st, and 28th days.Results: The control group of rats showed no variation. The diabetic control rats showed consistent hyperglycemia. Comparing the test drug Caesalpinia bonduc to the standard drug Glibenclamide, the test drug was 1.38 times more efficacious than the standard.Conclusions: The alcoholic extract of Caesalpinia bonduc (seeds) has shown more anti diabetic activity by lowering the blood glucose levels in diabetic rats significantly. These findings suggest that hypoglycemic potential of the test compound Caesalpinia bonduc is promising and found to be more significant than the standard compound

Type 2 diabetic neuropathy with special reference to mitochondrial role and its effective management

Diabetic neuropathy denotes to a group of nerve disorders caused by diabetes. It may occur in both type 1 and type 2 diabetes patients. Mitochondria are essential for energy production as well as intermediary metabolism and equally important in the action of insulin on its targeted tissue. Recently, mitochondrial dysfunctions have been recognized as a cause of diabetes. Hyperglycemia enhances the activity of mitochondrial electron transport chain, leading to mitochondrial hyperpolarisation and elevates the reactive oxygen species (ROS) production. Increased electron availability causes partial reduction of oxygen to superoxide in the proximal electron transport chain which subsequently induces neurodegeneration in diabetes. Currently there is no satisfactory pharmacotherapy for painful diabetic neuropathy. This review summarizes mitochondrial role in type 2 diabetic neuropathy, diagnostic challenges, general treatments and benefits of alternative approach for effective management

Protective Effects of Bogijetong Decoction and Its Selected Formula on Neuropathic Insults in Streptozotocin-Induced Diabetic Animals

Bogijetong decoction (BGJTD) is a mixture of herbal formulation which is used in the traditional Korean medicine for the treatment of neuropathic pain caused by diabetes. Here, we investigated the regulatory effects of BGJTD and its reconstituted decoction subgroups on the neuropathic responses in streptozotocin- (STZ-) induced diabetic animals. Be decoction (BeD) was formulated by selecting individual herbal components that induced neurite outgrowth most efficiently in each subgroup. BeD induced the neurite outgrowth in DRG neurons most efficiently among decoction subgroups and downregulated the production of TNF-α from the sciatic nerves in STZ-diabetic animals. While the levels of phospho-Erk1/2 were elevated in the sciatic nerves of STZ-diabetic animals by BGJTD and BeD treatments, p38 level was downregulated by BGJTD and BeD. A single herbal component of BeD induced neurite outgrowth comparable to BeD and was involved in the regulation of Erk1/2 activation and TNF-α production in DRG neurons. Oral administration of BGJTD and BeD in STZ-diabetic animals reduced the latency time responding to thermal stimulation. Our results suggest that the reconstituted formulation is as effective as conventional BGJTD in inducing biochemical and behavioral recoveries from the neuropathy in peripheral nerves and thus the experimental reductionism may be applied to develop the methodology for compositional analysis of herbal decoctions

Vascular Contributions to Cognitive Impairment and Treatments with Traditional Chinese Medicine

published_or_final_versio

The emerging role of TXNIP in ischemic and cardiovascular diseases; a novel marker and therapeutic target

Thioredoxin interacting protein (TXNIP) is a metabolism-oxidative-and inflammation-related marker induced in cardiovascular diseases and is believed to represent a possible link between metabolism and cellular redox status. TXNIP is a potential biomarker in cardiovascular and ischemic diseases but also a novel identified target for preventive and curative medicine. The goal of this review is to focus on the novelties concerning TXNIP. After an overview in TXNIP involvement in oxidative stress, inflammation and metabolism, the remainder of this review presents the clues used to define TXNIP as a new marker at the genetic, blood, or ischemic site level in the context of cardiovascular and ischemic diseases

Isorhamnetin: A review of pharmacological effects.

Isorhamnetin is one of the most important active ingredients in the fruits of Hippophae rhamnoides L. and the leaves of Ginkgo biloba L., which possesses extensive pharmacological activities. At present, there have been numerous investigations on isorhamnetin, which has the effects of cardiovascular and cerebrovascular protection, anti-tumor, anti-inflammatory, anti-oxidation, organ protection, prevention of obesity, etc. The related mechanisms involve the regulation of PI3K/AKT/PKB, NF-κB, MAPK and other signaling pathways as well as the expression of related cytokines and kinases. Isorhamnetin has a high value of development and application. However, the investigations on its mechanism of action are limited and lack of detailed scientific validation. The manuscript reviewed the pharmacological effects of isorhamnetin and related mechanisms of action for the development of its medicinal properties further