A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice

Background: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed. Objective: The main objective was to investigate the ability of genetically engineered Camelina sativa (20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals. Methods: Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil–containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined. Results: The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P < 0.001). Significantly lower (36–38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P < 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P < 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P < 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P < 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator–activated receptor α (Ppara), and peroxisome proliferator–activated receptor γ (Pparg) (P < 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident. Conclusions: Oil from transgenic Camelina is a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial

Implementation of a Workplace-Based Assessment to Measure Performance of the Core Entrustable Professional Activities in the Pediatric Clerkship

Background: In 2013, the AAMC convened a panel of medical education experts to delineate the 13 Core Entrustable Professional Activities (Core EPAs) medical school graduates should be able to perform without direct supervision by day one of their residency. 1 Assessment of these EPAs requires direct observation to render entrustment decisions. 2 As part of our engagement in the AAMC Core EPA pilot,3 we created a workplace-based assessment (WBA) system to assist in measurement of student performance for the Core EPAs at the Virginia Commonwealth University School of Medicine. For the Pediatrics clerkship, we identified 6 Core EPAs to assess for all students during the 2018-2019 academic year: -Core EPA 1 (history and physical exam) -Core EPA 2 (prioritize a differential diagnosis) -Core EPA 3 (recommend and interpret common diagnostic and screening tests) -Core EPA 5 (document clinical encounter in patient record) -Core EPA 6 (provide an oral presentation of a clinical encounter) -Core EPA 9 (collaborate as a team member of an interprofessional team)

Investigation of Polymer–Plasticizer Blends as SH-SAW Sensor Coatings for Detection of Benzene in Water with High Sensitivity and Long-Term Stability

We report the first-ever direct detection of benzene in water at concentrations below 100 ppb (parts per billion) using acoustic wave (specifically, shear-horizontal surface acoustic wave, SH-SAW) sensors with plasticized polymer coatings. Two polymers and two plasticizers were studied as materials for sensor coatings. For each polymer–plasticizer combination, the influence of the mixing ratio of the blend on the sensitivity to benzene was measured and compared to commercially available polymers that were used for BTEX (benzene, toluene, ethylbenzene, and xylene) detection in previous work. After optimizing the coating parameters, the highest sensitivity and lowest detection limit for benzene were found for a 1.25 μm thick sensor coating of 17.5%-by-weight diisooctyl azelate-polystyrene on the tested acoustic wave device. The calculated detection limit was 45 ppb, with actual sensor responses to concentrations down to 65 ppb measured directly. Among the sensor coatings that showed good sensitivity to benzene, the best long-term stability was found for a 1.0 μm thick coating of 23% diisononyl cyclohexane-1,2-dicarboxylate-polystyrene, which was studied here because it is known to show no detectable leaching in water. The present work demonstrates that, by varying type of plasticizer, mixing ratio, and coating thickness, the mechanical and chemical properties of the coatings can be conveniently tailored to maximize analyte sorption and partial chemical selectivity for a given class of analytes as well as to minimize acoustic-wave attenuation in contact with an aqueous phase at the operating frequency of the sensor device

Climate Change and the Clean Air Act

In Massachusetts v. EPA, petitioners - twelve states, three cities, an American territory, and numerous health and environmental groups - have asked the Supreme Court to hold that the Clean Air Act gives EPA the power to regulate greenhouse gas emissions from motor vehicles and that EPA may not decline to exercise this power based on statutorily irrelevant factors. The problem petitioners ultimately seek to address - climate change - is unique in its scope and complexity. But the legal issues before the Court in Massachusetts v. EPA are neither particularly grand nor particularly complex. They are the kinds of statutory and administrative law issues courts address every day in this country without great trouble or fanfare. My aim in this Article is to show that a standard approach to the legal issues raised in Massachusetts v. EPA dictates a ruling in petitioners\u27 favor

An investigation into the effects of Omega-3 fatty acids on bone resorption in the female ovariectomised rat : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Nutritional Science at Massey University

Estrogen deficiency results in disruption of the normal bone remodeling cyclc leading to a loss of bone mineral and, in many cases, the development of osteoporosis. Various studies have demonstrated a beneficial effect of essential fatty acids (EFAs) in reducing the loss of bone density as a consequence of estrogen deficiency. The aim of the present study was to examine the specific effects of the n-3 EFA, eicosopentaenoic acid (EPA) on bone density and strength in ovariectomised female rats. 60 Sprague-Dawley rats were randomized into four groups and either ovariectomised (n=45) or sham operated (n=15). Ovariectomised animals were fed calcium adequate diets containing either corn oil (OVX control, n=15), corn oil + 0.1g/kg body weight EPA (low dose, n=15) or corn oil + 1.0g/kg body weight EPA (high dose, n= 15) for a period of nine weeks. Sham rats were fed the corn oil diet as per the OVX control group. Urinary calcium and phosphate excretion, serum type 1 collagen c-telopeptide concentration, bone density, bone ash and bone breaking strength were measured. Plasma fatty acid composition and serum concentrations of 25 hydroxyvitamin D3 were also determined. Femur bone density was significantly lower in the high dose group compared to sham, OVX control and low dose EPA groups (p<0.001, p=0.0096 and p=0.0047 respectively). Low dose EPA supplementation had no significant effect on bone density. No significant differences in urinary calcium or phosphate concentrations, serum concentrations of type-1 collagen c-telopeptide or bone breaking strength were evident with either dose of EPA compared to unsupplemented, ovariectomised controls. EPA supplementation resulted in significant decreases in the levels of n-6 EFAs and increases in the levels of n-3 EFAs except docosahexaenoic acid in plasma lipids. Both low and high dose EPA supplementation led to significant increases in serum concentration of25(OH) vitamin D3. In conclusion 1.0g EPA/kg body weight had a detrimental effect on bone density in ovariectomised rats. It is proposed that high intake of the highly unsaturated EPA resulted in significant lipid peroxidation. This in turn disrupted membrane structure and inhibited intestinal calcium absorption thereby stimulating PTH-mediated bone resorption. potential role for n-3 EFAs in the regulation of vitamin D activity is also outlined

The Effects of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) on Brown Adipogenesis in Stem Cell Culture

Polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are major maternal dietary supplements due to their positive benefits on neurological tissue growth during the first 12 weeks of gestation. Previous studies show that EPA and DHA inhibit muscle formation but promote adipogenesis. However, no research has addressed the question whether high intake of EPA and DHA affects brown fat development during gestation. The objective of this study was to measure the effect of EPA and DHA supplement on brown adipogenesis and potential pathways related to mitochondrial biosynthesis using fibroblasts as in vitro model. Using Oil-Red-O staining and PCR testing, lipid droplet formation and tested six genes were examined and PGC1α presented statistically significant difference from the control group when treated with PUFAs. Results indicated that PGC1α gene expression can be to be alternated by EPA and DHA treatment. Mitochondrial biosynthesis can potentially be promoted by increased PGC1α gene expression. However, the lipid droplets accumulated in the PUFAs treated group show an unknown mechanism of the n-3 PUFA on adipogenesis that needs to be revealed

The Effects of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) on Brown Adipogenesis in Stem Cell Culture

Polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are major maternal dietary supplements due to their positive benefits on neurological tissue growth during the first 12 weeks of gestation. Previous studies show that EPA and DHA inhibit muscle formation but promote adipogenesis. However, no research has addressed the question whether high intake of EPA and DHA affects brown fat development during gestation. The objective of this study was to measure the effect of EPA and DHA supplement on brown adipogenesis and potential pathways related to mitochondrial biosynthesis using fibroblasts as in vitro model. Using Oil-Red-O staining and PCR testing, lipid droplet formation and tested six genes were examined and PGC1α presented statistically significant difference from the control group when treated with PUFAs. Results indicated that PGC1α gene expression can be to be alternated by EPA and DHA treatment. Mitochondrial biosynthesis can potentially be promoted by increased PGC1α gene expression. However, the lipid droplets accumulated in the PUFAs treated group show an unknown mechanism of the n-3 PUFA on adipogenesis that needs to be revealed

Putting Community First: A Promising Approach to Federal Collaboration for Environmental Improvement: An Evaluation of the Community Action for a Renewed Environment (CARE) Demonstration Program

This report is an independent evaluation of the Environmental Protection Agency's (EPA) Community Action for a Renewed Environment (CARE) Demonstration Program, a community-driven process that uses the best available data to help communities set priorities and take action on their greatest environmental risks. CARE fosters local partnerships that seek participation from business, government, organizations, residents and EPA staff. It also supports a public, transparent planning and implementation process based on collaborative decision-making and shared action.Key FindingsThe National Academy Panel overseeing this effort was impressed by the dedication of the EPA staff to this unique initiative and commended the EPA for its efforts to partner with communities in achieving important long-term and sustainable environmental improvements at the local level. Recommended actions for the CARE Program include: (1) develop and implement a multifaceted information sharing approach; (2) coordinate and refine internal program management activities; and (3) develop a strategic plan and a business plan for CARE

Eicosapentaenoic acid and oxypurinol in the treatment of muscle wasting in a mouse model of cancer cachexia

Cancer cachexia is a wasting condition, driven by systemic inflammation and oxidative stress. This study investigated eicosapentaenoic acid (EPA) in combination with oxypurinol as a treatment in a mouse model of cancer cachexia. Mice with cancer cachexia were randomized into 4 treatment groups (EPA (0.4 g/kg/day), oxypurinol (1 mmol/L ad-lib), combination, or control), and euthanized after 29 days. Analysis of oxidative damage to DNA, mRNA analysis of pro-oxidant, antioxidant and proteolytic pathway components, along with enzyme activity of pro- and antioxidants were completed on gastrocnemius muscle. The control group displayed earlier onset of tumor compared to EPA and oxypurinol groups (P&lt;0.001). The EPA group maintained body weight for an extended duration (20 days) compared to the oxypurinol (5 days) and combination (8 days) groups (P&lt;0.05). EPA (18.2&plusmn;3.2 pg/ml) and combination (18.4&plusmn;3.7 pg/ml) groups had significantly higher 8-OH-dG levels than the control group (12.9&plusmn;1.4 pg/ml, P&le;0.05) indicating increased oxidative damage to DNA. mRNA levels of GPx1, MURF1 and MAFbx were higher following EPA treatment compared to control (P&le;0.05). Whereas oxypurinol was associated with higher GPx1, MnSOD, CAT, XDH, MURF1, MAFbx and UbB mRNA compared to control (P&le;0.05). Activity of total SOD was higher in the oxypurinol group (32.2&plusmn;1.5 U/ml) compared to control (27.0&plusmn;1.3 U/ml, P&lt;0.01), GPx activity was lower in the EPA group (8.76&plusmn;2.0 U/ml) compared to control (14.0&plusmn;1.9 U/ml, P&lt;0.05), and catalase activity was lower in the combination group (14.4&plusmn;2.8 U/ml) compared to control (20.9&plusmn;2.0 U/ml, P&lt;0.01). There was no change in XO activity. The increased rate of weight decline in mice treated with oxypurinol indicates that XO may play a protective role during the progression of cancer cachexia, and its inhibition is detrimental to outcomes. In combination with EPA, there was little significant improvement from control, indicating oxypurinol is unlikely to be a viable treatment compound in cancer cachexia.<br /