Imaging of RNA in situ hybridization by atomic force microscopy

In this study we investigated the possibility of imaging internal cellular molecules after cytochemical detection with atomic force microscopy (AFM). To this end, rat 9G and HeLa cells were hybridized with haptenized probes for 28S ribosomal RNA, human elongation factor mRNA and cytomegalovirus immediate early antigen mRNA. The haptenized hybrids were subsequently detected with a peroxidase-labelled antibody and visualized with 3,3'-diaminobenzidine (DAB). The influence of various scanning conditions on cell morphology and visibility of the signal was investigated. In order to determine the influence of ethanol dehydration on cellular structure and visibility of the DAB precipitate, cells were kept in phosphate-buffered saline (PBS) and scanned under fluid after DAB development or dehydrated and subsequently scanned dry or submerged in PBS. Direct information on the increase in height of cellular structures because of internally precipitated DAB and the height of mock-hybridized cells was available. Results show that internal DAB precipitate can be detected by AFM, with the highest sensitivity in the case of dry cells. Although a relatively large amount of DAB had to be precipitated inside the cell before it was visible by AFM, the resolution of AFM for imaging of RNA–in situ hybridization signals was slightly better than that of conventional optical microscopy. Furthermore, it is concluded that dehydration of the cells has irreversible effects on cellular structure. Therefore, scanning under fluid of previously dehydrated samples cannot be considered as a good representation of the situation before dehydration.\ud \u

Lattice Gas Automata for Reactive Systems

Reactive lattice gas automata provide a microscopic approachto the dynamics of spatially-distributed reacting systems. After introducing the subject within the wider framework of lattice gas automata (LGA) as a microscopic approach to the phenomenology of macroscopic systems, we describe the reactive LGA in terms of a simple physical picture to show how an automaton can be constructed to capture the essentials of a reactive molecular dynamics scheme. The statistical mechanical theory of the automaton is then developed for diffusive transport and for reactive processes, and a general algorithm is presented for reactive LGA. The method is illustrated by considering applications to bistable and excitable media, oscillatory behavior in reactive systems, chemical chaos and pattern formation triggered by Turing bifurcations. The reactive lattice gas scheme is contrasted with related cellular automaton methods and the paper concludes with a discussion of future perspectives.Comment: to appear in PHYSICS REPORTS, 81 revtex pages; uuencoded gziped postscript file; figures available from [email protected] or [email protected]

Effects of stress on the immune system of fish

The effects of stress on the immune system of various fish species including dab Limanda limanda, flounder Platichthys flesus, sea bass Dicentrarchus labrax and gobies Zosterisessor ophiocephalus, were investigated from laboratory and field experiments, using various assays to measure immunocompetence, correlated with histological and ultrastructural observations. Modulation of the immune system was demonstrated at tissue, cellular and biochemical levels following exposure to various stressors. The spleen somatic index was depressed in dab stressed in the laboratory and gobies collected from polluted sites in the Venice Lagoon. Differential blood cell counts consistently showed an increase in phagocytes and decrease in thrombocytes in fish exposed to various stressors. Phagocytic activity from spleen and kidney adherent cells was stimulated in dab stressed by transportation but depressed in fish exposed to chemical pollutants. Respiratory burst activity in phagocytic cells was also stimulated in stressed dab but depressed in sea bass exposed to cadmium. The results are discussed in relation to current concepts on stress in fish and the regulation of the immune system

Generalized small-signal modelling of dual active bridge DC/DC converter

this paper presents a novel generalised approach of the small-signal modelling of dual active bridge (DAB) DC/DC converter. The adopted analysis is based on a per-unit fundamental frequency representation of the DAB. The outcome of the proposed modelling approach is a small signal, linearised, state-space DAB model; which is considered as a main building block for future control applications. The developed small signal DAB model includes all possible degrees of freedom affecting the performance of the DAB; this includes the voltage conversion ratio to allow the study of all DAB operation modes (i.e.: unity-gain and buck/boost modes.). Furthermore, since triple phase shift control (TPS) is used in this development work, the proposed model incorporates phase shift in addition to duty ratios. This feature allows for bridge voltage regulation, which is essential for efficient DAB operation in the case of buck/boost operation. Another key achievement is that the proposed small signal modelling methodology can be applied to any bidirectional DC-DC converter regardless of ratings, parameter values and number of ports. Extensive simulation is carried out to verify the proposed analysis

Changes of fatty acid metabolism and oxidative phosphorylation of rat liver mitochondria during 3'-Me-DAB feeding

Respiration, activity of oleate oxidation and composition of the total fatty acids of rat liver were investigated in 3'-Me-DAB feeding. 1. Oxidative phosphorylation of rat liver mitochondria decreased temporarily at relatively earlier stages (about 2 to 3 weeks) in 3'-Me-DAB feeding. 2. The activity of oleate oxidation of rat liver mitochondria decreased rapidly to about one third of that in control groups after the start of 3'-Me-DAB feeding. 3. In the composition of the total fatty acids of rat liver, the proportion of oleic acid increased in 3'-Me-DAB groups. 4. Unknown octadecamonoenoic acid was observed in liver mitochondria of rat fed on 3'-Me-DAB. 5. Proportions of oleic and palmitoleic acids in liver tumors and mitochondria of liver tumors induced by 3'-Me-DAB feeding increased remarkably in contrast with decrease in those of palmitic and eicosapolyenoic acids. 6. A possibility was discussed about how higher level of oleate in the liver cells in azo dye feeding may be concerned with the tumor induction.</p

Reactive power minimization of dual active bridge DC/DC converter with triple phase shift control using neural network

Reactive power flow increases dual active bridge (DAB) converter RMS current leading to an increase in conduction losses especially in high power applications. This paper proposes a new optimized triple phase shift (TPS) switching algorithm that minimizes the total reactive power of the converter. The algorithm iteratively searches for TPS control variables that satisfy the desired active power flow while selecting the operating mode with minimum reactive power consumption. This is valid for the whole range of converter operation. The iterative algorithm is run offline for the entire active power range (-1pu to 1pu) and the resulting data is used to train an open loop artificial neural network controller to reduce computational time and memory allocation necessary to store the data generated. To validate the accuracy of the proposed controller, a 500-MW 300kV/100kV DAB model is simulated in Matlab/Simulink, as a potential application for DAB in DC grids

Experimental comparison between proportional and PWM-solenoid valves controlled servopneumatic positioning systems

The performance of the Dynamical Adaptive Backstepping-Sliding Mode Control (DAB-SMC) scheme for positioning of a pneumatic cylinder regulated by two types of PWM-solenoid valves is experimentally investigated. The goal is to study the compromise in controller’s performance as the system moves from using a proportional valve to employing the low-cost PWM-solenoid valves. Sinusoidal and multiple-step inputs are used as the reference position trajectories. Experimental results show that the DAB-SMC scheme works best with the proportional valve. The performance, however, deteriorates by more than twofold, once the system utilizes PWM- solenoid valves of 3/2-way or 2/2-way configurations. From this study, tradeoff between performances of different types of valves applied on a DAB-SMC scheme-controlled servo positioning system is successfully documented. This information helps to configure appropriate servopneumatic system for positioning applications

Generic closed loop controller for power regulation in dual active bridge DC-DC converter with current stress minimization

This paper presents a comprehensive and generalized analysis of the bidirectional dual active bridge (DAB) DC/DC converter using triple phase shift (TPS) control to enable closed loop power regulation while minimizing current stress. The key new achievements are: a generic analysis in terms of possible conversion ratios/converter voltage gains (i.e. Buck/Boost/Unity), per unit based equations regardless of DAB ratings, and a new simple closed loop controller implementable in real time to meet desired power transfer regulation at minimum current stress. Per unit based analytical expressions are derived for converter AC RMS current as well as power transferred. An offline particle swarm optimization (PSO) method is used to obtain an extensive set of TPS ratios for minimizing the RMS current in the entire bidirectional power range of - 1 to 1 per unit. The extensive set of results achieved from PSO presents a generic data pool which is carefully analyzed to derive simple useful relations. Such relations enabled a generic closed loop controller design that can be implemented in real time avoiding the extensive computational capacity that iterative optimization techniques require. A detailed Simulink DAB switching model is used to validate precision of the proposed closed loop controller under various operating conditions. An experimental prototype also substantiates the results achieved

Replication and discovery of musculoskeletal QTLs in LG/J and SM/J advanced intercross lines

AR056280 awarded to DAB and AL. AIHC supported by IMS and Elphinstone Scholarship from the University of Aberdeen. GRV supported by Medical Research Scotland (Vac-929-2016).Peer reviewedPublisher PD