Lipid Metabolism

Perilipin 2, also known as Adipose differentiation-relation protein or PLIN2, is a lipid droplet-binding protein present in almost every tissue. The absence of PLIN2 upregulates hepatic very low-density lipoprotein secretion, relieves hepatosteatosis, and improves whole body insulin resistance in mice. Despite of the importance in mediating lipid metabolism, the regulation of PLIN2 itself remains largely unknown. Previous reports have shown that X-box binding protein 1 (XBP1) is an important regulator of lipogenesis. XBP1 is a transcription factor that recognizes and binds to a consensus sequence, 5’-TGACGTGG-3’. Interestingly, when we looked through the promoter region of mouse Plin2 gene, we found that the consensus sequence is present in the Plin2 promoter. Therefore, we hypothesize that XBP1 might directly bind to Plin2 promoter and regulate the Plin2 expression. To test our hypothesis, we will perform the luciferase assay to examine whether the Plin2 promoter activity is regulated by XBP1. We first designed forward and reverse PCR primers, which include BglII and BamHI restriction enzyme sites respectively, to amplify the Plin2 promoter region (from -1100 to +40). We performed PCR and cloned the Plin2 promoter to a TA vector. The TA vector was then sequenced to exclude any point mutations. After sequencing, we sub cloned the Plin2 promoter into a vector containing a luciferase reporter. In the future, we will transfect 293T, a human embryonic kidney cell line, with the Plin2 promoter-luciferase vector we generated. We will compare the Plin2 promoter activity by measuring the luminescence in the presence or absence of XBP1

Chloroquine-induced bull's eye maculopathy

We report the case of a 51-year-old woman who presented with bilateral progressive deterioration in vision after taking chloroquine for severe rheumatoid arthritis for 10 years. She was found to have a bull's eye pattern of depigmentation in the macula of both eyes. Despite cessation of chloroquine, her vision did not improve. The clinical presentation of chloroquine retinopathy is discussed, along with the importance of scheduled eye examination for individuals taking chloroquine or hydroxychloroquine.published_or_final_versio

Flashes, floaters, and retinal detachment

Patients presenting to the family physician and ophthalmologist often have complaints of flashes and floaters. These symptoms may be benign or they may be associated with serious conditions such as a retinal tear or retinal detachment. Recognizing the importance of these symptoms would allow prompt referral to the ophthalmologist for a detailed ocular examination. Today, more than 95% of retinal detachments can be successfully treated, although more than one procedure may be required. Prevention or early diagnosis is important because the rate of successful repair is higher and the visual results are better if the retinal detachment spares the macula, the part of the retina responsible for fine, central vision. Through successful treatment, patients will be able to enjoy a better quality of life and maintain their abilities to read, work, drive, and care for themselves.published_or_final_versio

DSC Dispatch, September 2012, Vol. 6

A quarterly publication by the Division of Soil Conservatio

Settlement Laws of Eneolithic and Early Bronze Age Peasant Communities in the Fergana Valley

Annotation: Since the ancient stone age, people have tried to occupy different areas based on natural climatic conditions. They occupied places with comfortable living conditions as much as possible. In this article highlights of settlement laws of eneolithic and early bronze age peasant communities in the fergana valley