Hardware design of a portable medical device to measure the quadriceps muscle group after a total knee arthroplasty by EMG, LBIA and clinical score methods

El propòsit d'aquest projecte és el disseny del hardware d'un dispositiu mèdic portàtil per a mesurar senyals d'electromiografia (EMG) i bioimpedància localitzada (LBIA), que s'utilitzarà per avaluar la progressió de dues pròtesis de genoll (Medial-Pivot i Ultra- Congruent) en pacients operats d'una artroplàstia total de genoll per a l'hospital Germans Trias i Pujol de Badalona. Per això, s'ha realitzat un estudi complet sobre els senyals d'EMG i LBIA, per tal de definir les característiques necessàries de l'equip mèdic i poder optimitzar el disseny electrònic. Per l'adquisició de senyals EMG, s'ha dissenyat i simulat un sistema compost per diferents fases, que treballen independentment per adquirir, amplificar, filtrar i adaptar el senyal EMG pel seu futur processament digital. D'altra banda, per obtenir valors de la bioimpedància localitzada dels diferents músculs que conformen el quàdriceps, s'ha dissenyat un sistema compost per dos grans blocs; el primer bloc és l'etapa d'injecció, on es genera i s'injecta un senyal feble de corrent altern a la zona a mesurar, mentre que el segon bloc, és l'etapa d'adquisició de senyals. Aquest últim s'encarrega d'adquirir la diferència de voltatge produïda per la injecció de corrent al múscul (anteriorment mencionat) per després calcular la bioimpedància a partir de la llei d'ohm. Tots els senyals són digitalitzats mitjançant el microcontrolador STM32F407VG, que s'encarregarà de processar i aconseguir les dades claus per determinar quina de les deus pròtesis desenvolupa una millor funció mecànica i una millor adaptació biològica. És important remarcar que tot el disseny, sigui per a EMG o LBIA s'ha dut a terme de manera discreta sense fer servir Front-Ends comercials o integrats complexos més que l'amplificador d'instrumentació o ADC. En addició, el present treball inclou una primera estimació dels costos de producció i fabricació per a una sola unitat, càlculs de consums i funcionament (sorolls, CMRR del sistema i amplada de banda) i una simulació completa d'EMG i LBIA per observar com funciona i es du a terme cada etapa del circuit. Finalment, en tractar-se d'un equip mèdic, també s'ha revisat la normativa aplicable i se n'ha analitzat l'impacte ambiental, s'ha proposat i definit diferents punts per a futurs treballs, com podria ser la validació i testatge de l'equip, càlculs més aproximats de consums i perfilar la bill of materials (BOM) per a grans demandes de components.The purpose of this project is the hardware design of a portable medical device to measure electromyography (EMG) and localized bioimpedance (LBIA) signals, which will be used to evaluate the adaptability and progression of two knee prostheses (medial-pivot and ultra-congruent) in patients undergoing total knee arthroplasty at the Germans Trias i Pujol Hospital in Badalona. For this, the present work undercovers the relevant properties of the EMG and LBIA signals in order to define the characteristics of the medical equipment and thus optimize its electronic design. For the EMG measurements, a system made up of different stages has been designed and simulated. These phases work independently to acquire, amplify, filter, and adapt the EMG signal for its further digital processing. On the other hand, to obtain the bioimpedance values of different quadriceps muscles, a system composed of two large blocks has been designed; the first is the injection block, where a weak alternating current signal is generated and injected into the area to be measured, while the second block is the signal acquisition stage. The purpose of the latter is to acquire the voltage difference produced by the injection of current (mentioned above) and then obtain the bioimpedance from Ohm's law. All the signals are digitized from the STM32F407VG microcontroller, which will be in charge of processing and obtaining the key data to determine which of the two prostheses performs a better mechanical function and biological adaptation. It is important to note that the entire design, whether for EMG or LBIA, has been developed discreetly without using commercial Front-Ends or complex ICs other than the instrumentation amplifier or ADC. In addition, the thesis includes a first estimation of the production and manufacturing costs for a single unit, calculations of consumption and work operation (noise, CMRR of the system and bandwidth) and a complete simulation of EMG and LBIA to observe how it works on each stage for both circuits. Finally, as it is a medical device, the applicable regulations have also been reviewed and its environmental impact has been analysed. Additionally, different points have been proposed and defined for future work, such as the construction of the PCB and its respective validation, improving both the consumption calculations and the list of materials (BOM) for large component demands.El propósito de este proyecto es el diseño del Hardware de un dispositivo médico portátil para mediciones de electromiografía (EMG) y bioimpedancia localizada (LBIA), que se utilizará para estudiar la evolución de la adaptabilidad y funcionamiento de dos prótesis de rodilla (medial-pívot y ultracongruente) en pacientes operados de artroplastia total de rodilla en el Hospital Germans Trias i Pujol de Badalona. Para ello, se ha realizado un estudio exhaustivo sobre las propiedades de las señales de EMG y LBIA con la finalidad de definir las características del equipo médico y de esta forma, optimizar el diseño electrónico del mismo. Para la lectura de mediciones EMG, se ha diseñado y simulado un sistema constituido por distintas etapas, que trabajan independientemente para adquirir, amplificar, filtrar, y adaptarla señal EMG para su posterior procesado digital. Por otro lado, para obtener los valores de bioimpedancia de distintos músculos del cuádriceps, se ha diseñado un sistema compuesto por dos grandes bloques; el primero es el bloque de inyección, donde se genera y se inyecta una señal débil de corriente alterna en la zona a medir, mientras que el segundo bloque es la etapa de adquisición de señales. Esta última tiene como finalidad adquirir la diferencia de voltaje producido por la inyección de corriente (anteriormente mencionada) para después obtener la bioimpedancia a partir de la ley de ohm. Todas las señales son digitalizadas a partir del microcontrolador STM32F407VG, que se encargará de procesar y obtener los datos claves para determinar cuál de las dos prótesis desempeña una mejor función mecánica y adaptación biológica. Es importante remarcar que todo el diseño, ya sea para EMG o LBIA, se ha desarrollado de manera discreta sin usar Front-Ends comerciales o integrados complejos más que el amplificador de instrumentación o ADC. En adición, la tesis incluye una primera estimación de los costes de producción y fabricación para una sola unidad, cálculos de consumos y funcionamiento (ruidos, CMRR del sistema y ancho de banda) y una simulación completa de EMG y LBIA para observar cómo funciona y se desarrolla cada etapa de los distintos circuitos. Finalmente, al tratarse de un equipo médico, también se ha revisado la normativa aplicable y se ha analizado el impacto ambiental del mismo. Por último, se han propuesto y definido distintos puntos para futuros trabajos, como es la construcción de la PCB y su respectiva validación, realizar cálculos más aproximados de consumos y perfilar la lista de materiales (BOM) para grandes demandas de componentes

A novel readout method for focal plane array imaging in the presence of large dark current

This research was an investigation of a novel readout method for focal plane array (FPA) optical imaging, especially for very sensitive detectors with large dark current. The readout method is based on periodically blocking the optical input enabling the removal of the dark current integration from the output. The research demonstrated that it is feasible to modulate the optical input with the designed readout circuit and thus achieve longer signal integration time to enhance the signal-to-noise ratio. Study of a proposed circuit model showed that in theory the correlated readout method could increase the output voltage swing and reduce the noise level by attenuating low frequency noise, thereby effectively improving the FPA dynamic range. Circuits based on standard CMOS circuitry were designed, simulated by PSpice, fabricated using Orbit 2µm n-well technology, and tested with a PI-4000 system. In the circuit evaluation, the output noise due to the clock switching phenomena, the gate signal feedthrough and the charge relaxation, was considered to be the critical problem. The most promising design for minimizing this problem had a CMOS current steering circuit at the input of a high CMRR operational amplifier. Simulation and test results showed that a modified capacitive transimpedance amplifier (CTIA) could subtract dark current output and reduce the output signal due to any difference between the frequencies of the optical input modulation signal and the switch modulation signal. In conclusion, the correlated readout circuit was shown to be a promising approach for advancing FPA technology

Design of an Efficient Wall Adapter

This report presents a design for an efficient AC adapter that uses 85% less power than conventional adapters when idle, for an additional cost of only $1.21. The design exceeds the team\u27s initial targets of 75$1.30. The team logically derived the final polling design from three initially proposed solutions. This project addresses the inefficiencies of modern AC adapters, whose increased utilization has become an increasing detriment to both economy and environment

Low-Power Wireless Medical Systems and Circuits for Invasive and Non-Invasive Applications

Approximately 75% of the health care yearly budget of public health systems around the world is spent on the treatment of patients with chronic diseases. This, along with advances on the medical and technological fields has given rise to the use of preventive medicine, resulting on a high demand of wireless medical systems (WMS) for patient monitoring and drug safety research. In this dissertation, the main design challenges and solutions for designing a WMS are addressed from system-level, using off-the-shell components, to circuit implementation. Two low-power oriented WMS aiming to monitor blood pressure of small laboratory animals (implantable) and cardiac-activity (12-lead electrocardiogram) of patients with chronic diseases (wearable) are presented. A power consumption vs. lifetime analysis to estimate the monitoring unit lifetime for each application is included. For the invasive/non-invasive WMS, in-vitro test benches are used to verify their functionality showing successful communication up to 2.1 m/35 m with the monitoring unit consuming 0.572 mA/33 mA from a 3 V/4.5 V power supply, allowing a two-year/ 88-hour lifetime in periodic/continuous operation. This results in an improvement of more than 50% compared with the lifetime commercial products. Additionally, this dissertation proposes transistor-level implementations of an ultra-low-noise/low-power biopotential amplifier and the baseband section of a wireless receiver, consisting of a channel selection filter (CSF) and a variable gain amplifier (VGA). The proposed biopotential amplifier is intended for electrocardiogram (ECG)/ electroencephalogram (EEG)/ electromyogram (EMG) monitoring applications and its architecture was designed focused on improving its noise/power efficiency. It was implemented using the ON-SEMI 0.5 µm standard process with an effective area of 360 µm2. Experimental results show a pass-band gain of 40.2 dB (240 mHz - 170 Hz), input referred noise of 0.47 Vrms, minimum CMRR of 84.3 dBm, NEF of 1.88 and a power dissipation of 3.5 µW. The CSF was implemented using an active-RC 4th order inverse-chebyshev topology. The VGA provides 30 gain steps and includes a DC-cancellation loop to avoid saturation on the sub-sequent analog-to-digital converter block. Measurement results show a power consumption of 18.75 mW, IIP3 of 27.1 dBm, channel rejection better than 50 dB, gain variation of 0-60dB, cut-off frequency tuning of 1.1-2.29 MHz and noise figure of 33.25 dB. The circuit was implemented in the standard IBM 0.18 µm CMOS process with a total area of 1.45 x 1.4 mm^(2). The presented WMS can integrate the proposed biopotential amplifier and baseband section with small modifications depending on the target signal while using the low-power-oriented algorithm to obtain further power optimization

Design of a bandpass oculometer

A passive oculometer is a device that measures the position of the eye by utilizing the corneo-retinal potential (CRP) generated by the eye. In this thesis, the Bandpass Oculometer replaces the joystick of a personal computer, allowing the viewer to use a computer simply by moving his eyes. Low frequency noise, present in all electronic circuits, produces an offset from the reference point, the center of the screen. In previous lowpass oculometry studies at University of Central Florida, this offset caused the cursor to drift toward the edge of the field of view within a period of 30 seconds, decreasing the ability to precisely control the cursor on the monitor. The Bandpass Oculometer is different from its predecessors in that bandpass filtering is utilized. By filtering all frequencies except where eye movement occurs, low frequency drift noise and higher frequency interference signals are eliminated. Consequently, the viewer can achieve a reference with no offset and no drift, resulting in smoother tracking and better control of the computer. An additional improvement to previous oculometers is the new computer interface. A diode shaping network is used to simulate a joystick resistance, and an optocoupler relays the output current to the computer. The optocoupler electrically isolates the viewer from the computer, thus making the system safer to use

Low-power switched capacitor voltage reference

Low-power analog design represents a developing technological trend as it emerges from a rather limited range of applications to a much wider arena affecting mainstream market segments. It especially affects portable electronics with respect to battery life, performance, and physical size. Meanwhile, low-power analog design enables technologies such as sensor networks and RFID. Research opportunities abound to exploit the potential of low power analog design, apply low-power to established fields, and explore new applications. The goal of this effort is to design a low-power reference circuit that delivers an accurate reference with very minimal power consumption. The circuit and device level low-power design techniques are suitable for a wide range of applications. To meet this goal, switched capacitor bandgap architecture was chosen. It is the most suitable for developing a systematic, and groundup, low-power design approach. In addition, the low-power analog cell library developed would facilitate building a more complex low-power system. A low-power switched capacitor bandgap was designed, fabricated, and fully tested. The bandgap generates a stable 0.6-V reference voltage, in both the discrete-time and continuous-time domain. The system was thoroughly tested and individual building blocks were characterized. The reference voltage is temperature stable, with less than a 100 ppm/°C drift, over a --60 dB power supply rejection, and below a 1 [Mu]A total supply current (excluding optional track-and-hold). Besides using it as a voltage reference, potential applications are also described using derivatives of this switched capacitor bandgap, specifically supply supervisory and on-chip thermal regulation

High-precision fluorescence photometry for real-time biomarkers detection

Les derniers évènements planétaires et plus particulièrement l'avènement sans précédent du nouveau coronavirus augmente la demande pour des appareils de test à proximité du patient. Ceux-ci fonctionnent avec une batterie et peuvent identifier rapidement des biomarqueurs cibles. Pareils systèmes permettent aux utilisateurs, disposant de connaissances limitées en la matière, de réagir rapidement, par exemple dans la détection d'un cas positif de COVID-19. La mise en œuvre de l'élaboration d'un tel instrument est un projet multidisciplinaire impliquant notamment la conception de circuits intégrés, la programmation, la conception optique et la biologie, demandant tous une maîtrise pointue des détails. De plus, l'établissement des spécifications et des exigences pour mesurer avec précision les interactions lumière-échantillon s'additionnent au besoin d'expérience dans la conception et la fabrication de tels systèmes microélectriques personnalisés et nécessitent en elles-mêmes, une connaissance approfondie de la physique et des mathématiques. Ce projet vise donc à concevoir et à mettre en œuvre un appareil sans fil pour détecter rapidement des biomarqueurs impliqués dans des maladies infectieuses telles que le COVID-19 ou des types de cancers en milieu ambulatoire. Cette détection se fait grâce à des méthodes basées sur la fluorescence. La spectrophotométrie de fluorescence permet aux médecins d'identifier la présence de matériel génétique viral ou bactérien tel que l'ADN ou l'ARN et de les caractériser. Les appareils de paillasse sont énormes et gourmand énergétiquement tandis que les spectrophotomètres à fluorescence miniatuarisés disponibles dans le commerce sont confrontés à de nombreux défis. Ces appareils miniaturisés ont été découverts en tirant parti des diodes électroluminescentes (DEL) à semi-conducteurs peu coûteuses et de la technologie des circuits intégrés. Ces avantages aident les scientifiques à réduire les erreurs possibles, la consommation d'énergie et le coût du produit final utilisé par la population. Cependant, comme leurs homologues de paillasse, ces appareils POC doivent quantifier les concentrations en micro-volume d'analytes sur une large gamme de longueurs d'onde suivant le cadre d'une économie en ressources. Le microsystème envisagé bénéficie d'une approche de haute précision pour fabriquer une puce microélectronique CMOS. Ce procédé se fait de concert avec un boîtier personnalisé imprimé en 3D pour réaliser le spectrophotomètre à la fluorescence nécessaire à la détection quantitative d'analytes en microvolume. En ce qui a trait à la conception de circuits, une nouvelle technique de mise à auto-zeroing est appliquée à l'amplificateur central, celui-ci étant linéarisé avec des techniques de recyclage et de polarisation adaptative. Cet amplificateur central est entièrement différentiel et est utilisé dans un amplificateur à verrouillage pour récupérer le signal d'intérêt éclipsé par le bruit. De plus, l'augmentation de la sensibilité de l'appareil permet des mesures quantitatives avec des concentrations en micro-volume d'analytes ayant moins d'erreurs de prédiction de concentration. Cet avantage cumulé à une faible consommation d'énergie, un faible coût, de petites dimensions et un poids léger font de notre appareil une solution POC prometteuse dans le domaine de la spectrophotométrie de fluorescence. La validation de ce projet s'est fait en concevant, fabriquant et testant un prototype discret et sans fil. Son article de référence a été publié dans IEEE LSC 2018. Quant à la caractérisation et l'interprétation du prototype d'expériences in vitro à l'aide d'une interface MATLAB personnalisée, cet article a été publié dans IEEE Sensors journal (2021). Les circuits intégrés et les photodétecteurs ont été fabriqués ont été conçus et fabriqués par Cadence en 2019. Relativement aux solutions de circuit proposées, elles ont été fabriquées avec la technologie CMOS 180 nm et publiées lors de la conférence IEEE MWSCAS 2020. Tout comme cette dernière contribution, les expériences in vitro avec le dispositif proposé incluant la puce personnalisée et le boîtier imprimé en 3D ont été réalisés et les résultats électriques et optiques ont été soumis au IEEE Journal of Solid-State Circuits (JSSC 2022).The most recent and unprecedented experience of the novel coronavirus increases the demand for battery-operated near-patient testing devices that can rapidly identify the target biomarkers. Such systems enable end-users with limited resources to quickly get feedback on various medical tests, such as detecting positive COVID-19 cases. Implementing such a device is a multidisciplinary project dealing with multiple areas of expertise, including integrated circuit design, programming, optical design, and biology, each of which needs a firm grasp of details. Alongside the need for experience in designing and manufacturing custom microelectronic systems, establishing the specifications and requirements to precisely measure the light-sample interactions requires an in-depth knowledge of physics and mathematics. This project aims to design and implement a wireless point-of-care (POC) device to rapidly detect biomarkers involved in infectious diseases such as COVID-19 or different types of cancers in an ambulatory setting using fluorescence-based methods. Fluorescence spectrophotometry allows physicians to identify and characterize viral or bacterial genetic materials such as DNAs or RNAs. The benchtop devices that are currently available are bulky and power-hungry, whereas the commercially available miniaturized fluorescence spectrophotometers are facing many challenges. Many of these difficulties have been resolved in literature thanks to inexpensive semiconductor light-emitting diodes (LEDs) and integrated circuits technology. Such advantages aid scientists in decreasing the size, power consumption, and cost of the final product for end-users. However, like the benchtop counterparts, such POC devices must quantify micro-volume concentrations of analytes across a wide wave length range under an economy of resources. The envisioned microsystem benefits from a high-precision approach to fabricating a CMOS microelectronic chip combined with a custom 3D-printed housing. This implementation results in a fluorescence spectrophotometer for qualitative and quantitative detection of micro-volume analytes. In terms of circuit design, a novel switched-biasing ping-pong auto-zeroed technique is applied to the core amplifier, linearized with recycling and adaptive biasing techniques. The fully differential core amplifier is utilized within a lock-in amplifier to retrieve the signal of interest overshadowed by noise. Increasing the device's sensitivity allows quantitative measurements down to micro-volume concentrations of analytes with less concentration prediction error. Such an advantage, along with low-power consumption, low cost, low weight, and small dimensions, make our device a promising POC solution in the fluorescence spectrophotometry area. The approach of this project was validated by designing, fabricating, and testing a discrete and wireless prototype. Its conference paper was published in IEEE LSC 2018, and the prototype characterization and interpretation of in vitro experiments using a custom MATLAB interface were published in IEEE Sensors Journal (2021). The integrated circuits and photodetectors were designed and fabricated by the Cadence circuit design toolbox (2019). The proposed circuit solutions were fabricated with 180-nm CMOS technology and published at IEEE MWSCAS 2020 conference. As the last contribution, the in vitro experiments with the proposed device, including the custom chip and 3D-printed housing, were performed, and the electrical and optical results were submitted to the IEEE Journal of Solid-State Circuits (JSSC 2022)

An optogenetic headstage for optical stimulation and neural recording in life science applications

L'optogénétique est une nouvelle méthode de contrôle de l’activité neuronale dans laquelle la lumière est employée pour activer ou arrêter certains neurones. Dans le cadre de ce travail, un dispositif permettant l’acquisition de signaux neuronaux et conduisant à une stimulation optogénétique de façon multicanale et temps-réel a été conçu. Cet outil est muni de deux canaux de stimulation optogénétique et de deux canaux de lecture des signaux neuronaux. La source de lumière est une DEL qui peut consommer jusqu’à 150 milliampères. Les signaux neuronaux acquis sont transmis à un ordinateur par une radio. Les dimensions sont d’environ 20×20×15 mm3 et le poids est de moins de 7 grammes, rendant l’appareil utile pour les expériences sur les petits animaux libres. Selon nos connaissances actuelles, le résultat de ce projet constitue le premier appareil de recherche optogénétique sans-fil, compact offrant la capture de signaux cérébraux et la stimulation optique simultanée.Optogenetics is a new method for controlling the neural activity where light is used to activate or silence, with high spatial and temporal resolution, genetically light-sensitized neurons. In optogenetics, a light source such as a LED, targets light-sensitized neurons. In this work, a light-weight wireless animal optogenetic headstage has been designed that allows multi-channel simultaneous real-time optical stimulation and neural recording. This system has two optogenetic stimulation channels and two electrophysiological reading channels. The optogenetic stimulation channels benefit from high-power LEDs (sinking 150 milliamps) with flexible stimulation patterns and the recorded neural data is wirelessly sent to a computer. The dimensions of the headstage are almost 20×20×15 mm3 and it weighs less than 7 grams. This headstage is suitable for tests on small freely-moving rodents. To the best of our knowledge, this is the first reported fully wireless headstage to offer simultaneous multichannel optical stimulation along with multichannel neural recording capability