Living collections of botanic gardens as a means of ex situ conservation : a case study of African Violets (Saintpaulia) in Europe

In all plant conservation, the priority is to maintain wild populations in situ (on site), which means that plants are conserved within their natural environment. Sometimes, as in the case of severely endangered Saintpaulia H. Wendl. (African violets), this is however, no sufficient. In that case, ex situ (off site) conservation in the form of live and in vitro collections and seed banks is necessary. In recent years, the role of botanic gardens in conservation and reintroduction of threatened plants, has been increasingly recognized. Botanic gardens throughout the world possess large living collections of species and accessions, but only vague assumptions of the utility of them in ex situ conservation have been made thus far. Whole plants, when kept ex situ, have advantages in education, research and display. On the other hand, living collections have the disadvantage of high maintenance costs, including high spatial requirements. Thus, usually only one or few genotypes are represented. The goal of this study is to evaluate botanic garden live collections as a means of ex situ conservation with the genus Saintpaulia as a case study. As a result, an ex situ conservation plan for Saintpaulia is outlined. Workability of a network ex situ conservation activity in botanic gardens is also evaluated. Four of the five most important European Saintpaulia holders were chosen as target botanic gardens: Helsinki University Botanic Garden (Finland), The National Botanic Garden of Belgium, The Botanic Garden of Uppsala University (Sweden) and the Royal Botanic Gardens Edinburgh (UK). The wild Saintpaulia collections of the gardens were reviewed and the identifications checked. Botanic garden databases were examined to trace clone accessions. Leaf cuttings from wild-collected accessions were planted in Helsinki University Botanic Garden. Of the total 183 Saintpaulia accessions of the four target gardens 155 (85 %) were unique, and 126 of these were of known wild origin. They were chosen to ex situ conservation collection. Due to the varying quality of the data of origin of the accessions, five classes for the different quality of origin data were developed. European botanic garden living collections of Saintpaulia proved to be a workable base of ex situ conservation for the genus. The amount of space needed to conserve the ideal of at least 50 unique accessions of each of the 26 Saintpaulia taxa is best possible to organize with the network ex situ conservation programme: each accession will be stored in at least two botanic gardens, but no garden will have all the accessions. Saintpaulia is an ideal genus for living ex situ collections: it is beautiful and well-known, small-sized and easy to grow and propagate. New ex situ accessions will be collected from the wild and finally reintroduced to their natural habitats. Further research needs to be carried out to find out the proper seed banking mechanisms for probably orthodox but dust-like seeds of Saintpaulia species. The lack of research on the basic biology and the population ecology of the genus hampers effective conservation work. Collaboration with amenity horticulture and the home countries of Saintpaulia is planned to utilize the genetic diversity of wild African violets in breeding new cultivars

Tree collections of Auckland : biodiversity and management : a thesis presented in partial fulfilment of the requirements for the degree of Master of Applied Science in Landscape Management at Massey University, Palmerston North, New Zealand

Appendix 1: Personal Communications Contact List. Page 182 has been removed. Please consult the print copy held in the Library.Recent developments in New Zealand environmental legislation and government policies are based on an economic world-view where landscape is portrayed as a 'natural' resource. This paradigm largely ignores the human-made urban landscape where the highest proportion of population live. Our national legislation pays little attention to urban vegetation in general and exotic tree collections in particular. Research therefore has a vital role in highlighting the character and values of urban vegetation, and in analysing the current models used to manage its continued existence. This study examines the importance of charting and managing biodiversity and focuses on managed amenity and scientific tree collections in the Auckland region. This regional study seeks to characterise the biodiversity of tree collections in Auckland, and describe how landscape management can best contribute to their biological and human-use values. The literature review establishes the importance and values of trees and urban vegetation as critical to the fabric of human lifestyles and the ecology of the city. It then reviews the basis for the preservation of biodiversity in managed plant collections. Management principles and systems are reviewed along with the legislative context in Auckland. No mandate is established for exotic plant collection managment under the Resource Management Act, 1991 (RMA1991). Management of this resource is found to lack the focus and funding from which natural and rural biodiversity benefit under the RMA and other national legislation. A biodiversity survey of thirty-eight collections of trees characterises the biodiversity in the collections, and a survey of twelve tree collection managers provides data about the goals, practices and tools used in the management of those collections. Results reveal the diverse and unique flora of Auckland tree collections, and establish a baseline for comparison over time. The regional mix of native and exotic species found in Auckland collections is compared with other national plant surveys, and demonstrates differences consistent with regional climatic character and a differing research focus. The largest collection of trees and the most specialist genera collections were found at the Auckland Regional Botanic Garden, while the university of Auckland provides the most extensive range of scientific plant-collection facilities. There is evidence of sequential open space acquisition for tree collections in the Auckland region. Comparison of best management practices put forward in current literature with current practices of collection managers in Auckland reveals some significant issues. There is little evidence found to suggest strategic plant acquisition goal setting. It is also of concern that only half the collection managers used computerised plant record systems such as inventories and specialised database systems. Only one third of the managers had management plans, and therefore documented collection goals. There was no significant difference in the use of best management practices between the private and publicly owned collections. Well-managed collections are characterised by high or specialist tree species diversity, clear strategic goal setting and management planning, computerised plant record systems, adequate resources, appropriate staffing and the use of monitoring tools. Discussion of these research results leads to three recommendations. Firstly, the management of specific sites is discussed in light of a proposed model for plant collection management. Secondly, management policy for the regional urban Forest is recommended. Lastly, the finding that Auckland's exotic tree collections are a valuable resource worthy of protection, currently having little status under the law, leads to the recommendation for the empowering of a national body (central agency) with a mandate for the national coordination of botanic gardens and plant collections. This research therefore charts aspects of Auckland's urban biodiversity with reference to current legislation and management models. Its findings and recommendations are of importance to reviewers of national environmental legislation, regional policymakers and tree collection managers

The green-leaved variant of Eucalyptus largiflorens: a story involving hybridization and observant local people

Eucalyptus largiflorens (Black Box) is the most common tree in the Chowilla anabranch system on the Murray River floodplain. It typically has dull, glaucous, grey-green leaves. Occasional trees with smaller, glossy green leaves (Green Box) occur scattered amongst the Black Box. In areas with increasing salinity, they usually appear much healthier than adjacent, normal Black Box trees. Green Box plants are intermediate between normal Eucalyptus largiflorens plants and Eucalyptus gracilis plants in many morphological and allozyme characters, strongly suggesting that they are hybrids between those species. Green Box plants tolerate salinity better and use water more conservatively than normal Black Box plants, traits that they have probably inherited from Eucalyptus gracilis. In 1994, the Botanic Gardens of Adelaide used tissue culture and micropropagation to produce nearly 9,000 cloned Green Box plants which were planted out on Riverland floodplains. Since the 1990s, the high cost of producing clonal plants has meant that no further such plantings have occurred. Because Green Box plants can be a considerable distance from the nearest plants of one putative parent (Eucalyptus gracilis), more detailed studies could contribute to the existing work on such phantom hybrids

Hand pollination to increase seed-set of red helleborine Cephalanthera rubra in the Chiltern Hills, Buckinghamshire, England

In 2007 and in previous years, as part of ongoing attempts to improve red helleborine Cephalanthera rubra seed-set, hand pollination of florets has been undertaken at a small colony of this species in Buckinghamshire, southern England. Natural pollination rarely occurs (one mature pod recorded in 10 years) at this site. In 2007, hand pollination resulted in the production of four seed pods, of which one withered and died. Upon ripening, the three remaining pods were removed for attempted micropropagation of the seeds. Ongoing conservation management has probably benefited the solitary bee Chelostoma campanularum which now appears fairly plentiful at the site, but despite the presence of this red helleborine flower visitor, natural pollination remains virtually unrecorded at this locality; field observations suggest that C.campanularum is in fact probably not large enough to act as an effective red helleborine pollinator as it can slip in and out of the flowers without removing the pollinia, unlike it larger relative C.fuliginosum, absent from the UK but which is a known pollinator of red helleborine in continental Europe

Re-evaluating Student Treatments of Barkcloth Artefacts from the Economic Botany Collection, Royal Botanic Gardens, Kew

Since 1995 textile conservation students have treated 17 pieces of barkcloth from the Economic Botany Collection, Royal Botanic Gardens, Kew. A re-examination of 8 treated objects has enabled comparison of the effectiveness of interventive treatments. Humidification has been applied by varied techniques, all found to give consistent, lasting results. Structural support for tears again used varied materials: nylon net in the 1990s, Reemay, Japanese paper, and reworked fibres; all have proved stable, although in some cases acrylic paint had stiffened the material. The objects’ role as part of a study collection means that easy access is required; mounts were therefore designed to allow examination of objects with minimal handling, e.g. through the use of trays. Documentation was not always adequate for this re-evaluation exercise, lacking detail on adhesive preparation or colouring. Overall, past conservation was found to have lasted well, with the proviso that some storage solutions have had to be revisited in the light of use. Close collaboration between curators and conservators has been crucial to this success

Narcissus dubius, Narcisse Douteux

This species is endemic to southern France and eastern Spain. It is assessed as Least Concern because it has a widespread distribution, occurs in several protected areas throughout its range and its population is not expected to significantly decline in the near future. Further studies on the genetic structure of its population and trends in population size at global level are needed

Kindia (Pavetteae, Rubiaceae), a new cliff-dwelling genus with chemically profiled colleter exudate from Mt Gangan, Republic of Guinea

A new genus Kindia (Pavetteae, Rubiaceae) is described with a single species, Kindia gangan, based on collections made in 2016 during botanical exploration of Mt Gangan, Kindia, Republic of Guinea in West Africa. The Mt Gangan area is known for its many endemic species including the only native non-neotropical Bromeliaceae Pitcairnia feliciana. Kindia is the fourth endemic vascular plant genus to be described from Guinea. Based on chloroplast sequence data, the genus is part of Clade II of tribe Pavetteae. In this clade, it is sister to Leptactina sensu lato (including Coleactina and Dictyandra). K. gangan is distinguished from Leptactina s.l. by the combination of the following characters: its epilithic habit; several-flowered axillary inflorescences; distinct calyx tube as long as the lobes; a infundibular-campanulate corolla tube with narrow proximal section widening abruptly to the broad distal section; presence of a dense hair band near base of the corolla tube; anthers and style deeply included, reaching about mid-height of the corolla tube; anthers lacking connective appendages and with sub-basal insertion; pollen type 1; pollen presenter (style head) winged and glabrous (smooth and usually hairy in Leptactina); orange colleters producing a vivid red exudate, which encircle the hypanthium, and occur inside the calyx and stipules. Kindia is a subshrub that appears restricted to bare, vertical rock faces of sandstone. Fruit dispersal and pollination by bats is postulated. Here, it is assessed as Endangered EN D1 using the 2012 IUCN standard. High resolution LC-MS/MS analysis revealed over 40 triterpenoid compounds in the colleter exudate, including those assigned to the cycloartane class. Triterpenoids are of interest for their diverse chemical structures, varied biological activities, and potential therapeutic value