Assessing Positioning of Centriolar Satellites by the Golgi Apparatus

Centriolar satellites are small, membraneless, proteinaceous granules that surround the centrosome and pericentriolar material, trafficking throughout the cell. They aid in centrosome maturation through the transport of centrosomal proteins and in the nucleation of the primary cilium. They have also been implicated in autophagy and proteostasis in the cell at large. While they organize at the centrosome, the factors influencing satellite location within the cell as a whole are unclear. Satellites are known to localize to noncentrosomal MTOCs in cell lines lacking the centrosome, indicating additional factors play a role in their localization. It is unknown whether secondary MTOCs influence satellite position. The Golgi is the cellular center of post-translational modification and a secondary MTOC, nucleating its own microtubules for protein export. To examine a possible linkage between the Golgi apparatus and centriolar satellites, hTERT-RPE1 cells were immunofluorescently stained for satellites, the Golgi, the centrosome, and imaged on Western Washington University’s Leica Stellaris 8 confocal microscope. Loss of organization during mitosis of centriolar satellites and the Golgi was observed to coincide at the prometaphase/metaphase transition. Satellite distribution was then quantified using custom ImageJ macros. Satellite intensity at the centrosome was found to be lower by at least 12% in cells where the centrosome was spatially separated from the Golgi, across five replicates. Centrosome separation from the Golgi also induced a quantifiable directional shift in satellite distribution in the direction of the Golgi in satellites both localized to the centrosome and in the cytoplasm. Taken together, the data suggest a role for the Golgi apparatus in organizing a secondary population of centriolar satellites. (The document here is a placeholder for work to be published elsewhere, then linked to this page upon publication. A poster is also included in the file below.

Partitioning billionscale hypergraphs

In this thesis, we present a hypergraph partitioning algorithm that achieves both, fast partitioning with high locality. The idea is simple but effective: the algorithm grows k disjoint subgraphs based on the neighbourhood relation and the degree distribution in the hypergraph. We performed extensive experiments and showed that our algorithm leads to perfectly balanced partitions with improved locality compared to state-of-the-art, while matching the fast runtime of streaming hypergraph partitioners.Aufgrund wachsender Kommunikationsnetzwerke und der immer größer werdenden Datenmengen in sozialen Netzwerken hat sich die Nachfrage nach Graph-Verarbeitungs-Systemen deutlich erhöht. Fast alle modernen Kommunikationsnetzwerke, wie z.B. Whatsapp, Facebook oder Reddit bieten heutzutage Gruppenfunktionalitäten an, welche sich sehr einfach mit Hilfe von Hypergraphen modellieren lassen. Um diese großen Hypergraphen verarbeiten zu können, müssen diese durch Hypergraph-Partitionierung auf viele verschiedene Maschinen verteilt werden. Solche Partitionierungs-Algorithmen existieren bereits, bieten jedoch entweder eine schnelle Laufzeit und schlechte Ergebnisse oder gute Ergebnisse und eine schlechte Laufzeit. In dieser Arbeit wird ein neuartiger Partitionierungs-Algorithmus vorgestellt, welcher beides bietet, eine schnelle Laufzeit und gute Ergebnisse. Die dem Algorithmus zugrunde liegende Idee ist einfach aber effektiv: der Algorithmus baut k disjunkte Subgraphen anhand der Nachbarschafts-Information der einzelnen Knoten. Es wurden ausführliche Tests durchgeführt, die zeigen, dass die Ergebnisse dieses Algorithmus eine deutlich verbesserte Lokalität aufweisen im Vergleich zu bereits existierenden Algorithmen, wobei er dennoch eine bessere Laufzeit als diese aufweisen kann

THE EFFECT OF COOPERATIVE INTEGRATED READING AND COMPOSITION (CIRC) TECHNIQUE ON STUDENTS’ READING COMPREHENSION OF DESCRIPTIVE TEXT AT THE EIGHTH GRADE OF MTSS AL-HUDA PEKANBARU

Tujuan penelitian ini untuk mengetahui pengaruh penggunaan Teknik Cooperative Integrated Reading and Composition (CIRC) terhadap pemahaman membaca teks deskriptif siswa di kelas VIII MTSS Al-Huda Pekanbaru. Penelitian ini merupakan penelitian pra-esperimental. Sampel penelitian ini diambil secara purposive sampling. Populasi penelitian ini adalah siswa kelas VIII MTSS Al-Huda Pekanbaru. Teknik pengumpulan data menggunakan tes pilihan ganda. Peneliti menggunakan uji Independent Sample T-test dengan menggunakan SPSS versi 24 dan eta square untuk menganalisis data Berdasarkan analisis data rumus uji-t. Nilaisig sebesar 0,000 maka dapat dinyatakan 0,00<0,05 berarti hipotesis nol (Ho) ditolak. Sedangkan hipotesis alternatif (Ha) diterima. Dapat disimpulkan terdapat perbedaan yang signifikan penggunaan teknik Cooperative Integrated Reading and Composition (CIRC) terhadap pemahaman membaca teks deskriptif siswa kelas VIII di MTSS Al-Huda Pekanbaru

PROFIL SOFT SKILLS SISWA PESERTA ON THE JOB TRAINING BIDANG KEAHLIAN TEKNOLOGI PENGOLAHAN HASIL PERTANIAN DI SMK NEGERI 1 PANDAK

Penelitian ini bertujuan untuk mengetahui 1) profil soft skills siswa peserta OJT bidang keahlian TPHP pada aspek disiplin, tanggung jawab, kerjasama, kreativitas dan motivasi kerja, 2) Dimensi aspek soft skills yang paling menonjol pada siswa peserta OJT bidang keahlian TPHP. Penelitian ini merupakan penelitian deskriptif kuantitatif dengan lokasi penelitian di SMK N 1 Pandak. Populasi penelitian adalah siswa kelas XII bidang keahlian Teknologi Pengolahan Hasil Pertanian di SMK N 1 Pandak yang telah melaksanakan OJT sebanyak 60 siswa. Metode pengumpulan data dengan menggunakan angket. Uji validitas konstruk dengan para Ahli (expert judgement). Teknik analisis data dalam penelitian ini dengan analisis deskriptif. Hasil penelitian menunjukkan bahwa 1) profil soft skills siswa bidang keahlian Teknologi Pengolahan Hasil Pertanian dalam aspek soft skills disiplin, tanggung jawab, kerjasama, dan motivasi kerja masuk ke dalam kategori SANGAT BAIK. Sedangkan untuk aspek kreativitas masih masuk ke dalam kategori CUKUP, yang artinya dalam aspek soft skills kreativitas masih perlu adanya perbaikan dan peningkatan. 2) Dimensi aspek soft skills siswa yang paling menonjol yaitu pada aspek motivasi kerja dengan nilai rerata tertinggi dibandingkan dengan soft skills lainnya yaitu sebanyak 3,62

The 2005 - 2030 Harlingen-San Benito Metropolitan Transportation Plan

116 pages. Prepared with the Texas Department of Transportation, Federal Highway Administration, and Federal Transit Administration

Function of the anterior gradient protein family in cancer

Proteomic technologies verified Anterior Gradient 2, AGR-2, as a protein over-expressed in human cancers, including breast, prostate and oesophagus cancers, with the ability to inhibit the tumour suppressor protein p53. AGR-2 gene is a hormone responsive gene with an unexpected induction by the anti-cancer drug tamoxifen highlighting the proto-oncogenic role of this protein. Anterior Gradient-2 encodes one protein that gives rise to two forms· the full length and the mature one. Full length bears a leader sequence that leads the protein to secretion. Localization studies of both forms of AGR-2 were performed using fluorescence microscopy and subcellular fractionation, in order to determine in which compartment the protein functions. Localization mutants of the mature and full length protein determined the exact sequence required for certain localization patterns. Once localization was confirmed, the mechanism of how Anterior Gradient-2 localization within the cell can inhibit p53 was initiated. Furthermore, novel peptide aptamers that bound to the protein were cloned into GFP vectors and their effect on AGR-2 was investigated. AGR-3, another member of the family, was also examined in terms of localization and function in MCF-7 cells. Yeast two hybrid analysis has identified potential nuclear and cytoplasmic binding partners for AGR-2, essential for the upstream or downstream regulation of the AGR-2 pathway. In conclusion, we present data showing models of how the Anterior Gradient protein family might function as drug-resistance survival factor in cancer as well as a p53 inhibitor, suggesting a multi-potent role of its members when it comes to trafficking, cellular localization and activation or inhibition pathways in cancer