Algae Biofuel Triacylglyceride Transesterification Optimization

Algae biofuels may hold the key to solving the problem of fossil fuel consumption by being comparable in content, renewable, and carbon-neutral. Many biofuel researchers and corporations have undertaken to increase the production rate or capacity of triacylglycerides (TAG), the fat precursor to biodiesel fuel produced by algae, in algae cultures and published articles documenting their findings. This research is devoted to evaluating the effect of water that may be present in samples on the conversion efficiency of TAG into fatty acid methyl esters (FAME), commonly referred to as biodiesel. Therefore, that efficiency was studied to find the water content which optimizes the yield and determine if further drying of algae was necessary as an additional step in sample preparation. The results showed that the water content typically present in lyophilized algae samples is not sufficient to appreciably inhibit the reaction efficiency and necessitate extensive drying as a sample preparation step prior to transesterification

Algae for biofuel:will the evolution of weeds limit the enterprise?

Algae hold promise as a source of biofuel. Yet the manner in which algae are most efficiently propagated and harvested is different from that used in traditional agriculture. In theory, algae can be grown in continuous culture and harvested frequently to maintain high yields with a short turnaround time. However, the maintenance of the population in a state of continuous growth will likely impose selection for fast growth, possibly opposing the maintenance of lipid stores desiriable for fuel. Any harvesting that removes a subset of the population and leaves the survivors to establish the next generation may quickly select traits that escape harvesting. An understanding of these problems should help identify methods for retarding the evolution and enhancing biofuel production

The future of bioethanol

Yeasts have been domesticated by mankind before horses. After the mastering of alcoholic fermentation for centuries, yeasts have become the protagonist of one of the most important biotechnological industries worldwide: the production of bioethanol. This chapter will initially present some important challenges to be overcome in this industry, both in first and second generation biofuel production. Then, it will briefly revisit some advances obtained in recent years. Finally, it will present and discuss some opportunities, in the scope of metabolic engineering and synthetic biology, that will likely be present in the future of bioethanol

Artificial Photosynthesis Would Unify the Electricity-Carbohydrate-Hydrogen Cycle for Sustainability

Sustainable development requires balanced integration of four basic human needs – air (O2/CO2), water, food, and energy. To solve key challenges, such as CO2 fixation, electricity storage, food production, transportation fuel production, water conservation or maintaining an ecosystem for space travel, we wish to suggest the electricity-carbohydrate-hydrogen (ECHo) cycle, where electricity is a universal energy carrier, hydrogen is a clean electricity carrier, and carbohydrate is a high-energy density hydrogen (14.8 H2 mass% or 11-14 MJ electricity output/kg)carrier plus a food and feed source. Each element of this cycle can be converted to the other reversibly & efficiently depending on resource availability, needs, and costs. In order to implement such cycle, here we propose to fix carbon dioxide by electricity or hydrogen to carbohydrate (starch) plus ethanol by cell-free synthetic biology approaches. According to knowledge in the literature, the proposed artificial photosynthesis must be operative. Therefore, collaborations are urgently needed to solve several technological bottlenecks before large-scale implementation

Manual pressing of nannochloropsis oculata dried biomass for enhanced lipid extraction

Microalgae offer significant potential to produce high value products and biofuels, whilst simultaneously being used to bio-remediate water or capture carbon dioxide (CO2). Microalgal cell disruption processes are often necessary to increase lipid extraction from microalgae before conventional solvent extraction processes are used to isolate lipids. The extracted lipids can be processed to produce biofuels. The combinations of hydraulic pressing with liquid nitrogen (LN2) treatment were applied to samples of dried Nannochloropsis oculata in the presented study to enhance the cellular destruction and lipid yields. The results indicated higher lipid extraction with LN2 treatment (0.159 g/g dry algae) compared to the LN2 untreated samples (0.070 g/g dry algae). The corresponding cell disruptions were found to be seventy-eight and fifty percent, respectively, at the same 10 bar (145 psi) pressure level. The control sample (without any treatment) lipid yield was 0.006 g/g dry algae, while the lipid yield varied between 0.192-0.213 g/g dry algae with LN2 treated biomass with pressure loadings of 70-100 bar (1015-1450 psi) and with a corresponding cell disruption of 93-98 percent. The presence of palmitate, oleate and linoleate found in the fatty acid methyl ester composition of the extracted lipids, shows a favourable profile to produce biodiesel

Bilirubin oxidase from myrothecium verrucaria physically absorbed on graphite electrodes. Insights into the alternative resting from and the sources of activity loss

The oxygen reduction reaction is one of the most important chemical processes in energy converting systems and living organisms. Mediator-less, direct electro-catalytic reduction of oxygen to water was achieved on spectrographite electrodes modified by physical adsorption of bilirubin oxidases from Myrothecium verrucaria. The existence of an alternative resting form of the enzyme is validated. The effect on the catalytic cycle of temperature, pH and the presence of halogens in the buffer was investigated. Previous results on the electrochemistry of bilirubin oxidase and on the impact of the presence of halogens are reviewed and reinterpreted

Overcoming barriers to the implementation of alternative fuels for road transport in Europe.

The success of implementing alternative fuels for road transport depends on their cost, performance and reliability. This paper focuses on the use of natural gas and LPG, hydrogen and biofuels in Europe. A brief presentation is given of their technical development status, their market potential, and barriers to their implementation in various market segments. Some market barriers are common to many new technologies, and can be overcome through adequate policy measures at European level. Generally, a combination of policies is required, and a number of supporting measures increase their effectiveness. The following policies affecting energy use in transport are discussed: market incentives, policies targeting technology and vehicle efficiency, and overall system improvement

Application of electro-active biofilms

The concept of an electro-active biofilm (EAB) has recently emerged from a few studies that discovered that certain bacteria which form biofilms on conductive materials can achieve a direct electrochemical connection with the electrode surface using it as electron exchanger, without the aid of mediators. This electro-catalytic property of biofilms has been clearly related to the presence of some specific strains that are able to exchange electrons with solid substrata (eg Geobacter sulfurreducens and Rhodoferax ferrireducens). EABs can be obtained principally from natural sites such as soils or seawater and freshwater sediments or from samples collected from a wide range of different microbially rich environments (sewage sludge, activated sludge, or industrial and domestic effluents). The capability of some microorganisms to connect their metabolisms directly in an external electrical power supply is very exciting and extensive research is in progress on exploring the possibilities of EABs applications. Indeed, the best known application is probably the microbial fuel cell technology that is capable of turning biomass into electrical energy. Nevertheless, EABs coated onto electrodes have recently become popular in other fields like bioremediation, biosynthesis processes, biosensor design, and biohydrogen production