Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo

Incorporation of noncanonical amino acids into cellular proteins often requires engineering new aminoacyl-tRNA synthetase activity into the cell. A screening strategy that relies on cell-surface display of reactive amino acid side-chains was used to identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants that allow efficient incorporation of the methionine (Met) analog azidonorleucine (Anl). We demonstrate that the extent of cell-surface labeling in vivo is a good indicator of the rate of Anl activation by the MetRS variant harbored by the cell. By screening at low Anl concentrations in Met-supplemented media, MetRS variants with improved activities toward Anl and better discrimination against Met were identified

The Three Loop Equation of State of QED at High Temperature

We present the three loop contribution (order $e^4$) to the pressure of massless quantum electrodynamics at nonzero temperature. The calculation is performed within the imaginary time formalism. Dimensional regularization is used to handle the usual, intermediate stage, ultraviolet and infrared singularities, and also to prevent overcounting of diagrams during resummation.Comment: ANL-HEP-PR-94-02, SPhT/94-054 (revised final version

Nuclei as Laboratories: Nuclear Tests of Fundamental Symmetries

The prospect of a rare isosotope accelerator facility opens up possibilities for a new generation of nuclear tests of fundamental symmetries. In this talk, I survey the current landscape of such tests and discuss future opportunities that a new facility might present.Comment: To appear in proceedings of 3rd ANL/MSU/INT/JINA Theory Workshop, Argonne National Laboratory (April, 2006); 13 page

Non-leading Logarithms in Principal Value Resummation

We apply the method of principal value resummation of large momentum-dependent radiative corrections to the calculation of the Drell Yan cross section. We sum all next-to-leading logarithms and provide numerical results for the resummed exponent and the corresponding hard scattering function.Comment: 22 pages (LaTeX), 5 figures not included; available upon request from [email protected], ANL-HEP-PR-94-25, ITP-SB-94-3

Bottom Quark Cross Sections at Collider and Fixed-Target Energies at the SSC and LHC

Calculations of inclusive cross sections for the production of bottom quarks in proton-proton collisions are presented as a function of energy, transverse momentum, and Feynman $x_F$ for values of $\sqrt{s}$ from $100~$GeV to $40~$TeV. In addition, we provide simple parametrizations of our theoretical results that should facilitate estimates of rates, acceptances, and efficiencies of proposed new detectors.Comment: 6 pages plus 11 topdraw figures appended as ps-files(uuencoded), Latex, ANL-HEP-CP-93-63 & CERN-TH.6987/9