3 research outputs found
Analysis and control of genetic toggle switches subject to periodic multi-input stimulation
In this letter, we analyze a genetic toggle switch recently studied in the
literature where the expression of two repressor proteins can be tuned by
controlling two different inputs, namely the concentration of two inducer
molecules in the growth medium of the cells. Specifically, we investigate the
dynamics of this system when subject to pulse-width modulated (PWM) input. We
provide an analytical model that captures qualitatively the experimental
observations reported in the literature and approximates its asymptotic
behavior. We also discuss the effect that the system parameters have on the
prediction accuracy of the model. Moreover, we propose a possible external
control strategy to regulate the mean value of the fluorescence of the reporter
proteins when the cells are subject to such periodic forcing.Comment: Preprint accepted for publication on L-CSS (First submission
31.05.2018, accepted 21.08.2018
Ratiometric control for differentiation of cell populations endowed with synthetic toggle switches
We consider the problem of regulating by means of external control inputs the
ratio of two cell populations. Specifically, we assume that these two cellular
populations are composed of cells belonging to the same strain which embeds
some bistable memory mechanism, e.g. a genetic toggle switch, allowing them to
switch role from one population to another in response to some inputs. We
present three control strategies to regulate the populations' ratio to
arbitrary desired values which take also into account realistic physical and
technological constraints occurring in experimental microfluidic platforms. The
designed controllers are then validated in-silico using stochastic agent-based
simulations.Comment: Accepted to CDC'201