An Evaluation of Target Speech for a Nonaudible Murmur Enhancement System in Noisy Environments

Abstract-Nonaudible murmur (NAM) is a soft whispered voice recorded with NAM microphone through body conduction. NAM allows for silent speech communication as it makes it possible for the speaker to convey their message in a nonaudible voice. However, its intelligibility and naturalness are significantly degraded compared to those of natural speech owing to acoustic changes caused by body conduction. To address this issue, statistical voice conversion (VC) methods from NAM to normal speech (NAM-to-Speech) and to a whispered voice (NAM-toWhisper) have been proposed. It has been reported that these NAM enhancement methods significantly improve speech quality and intelligibility of NAM, and NAM-to-Whisper is more effective than NAM-to-Speech. However, it is still not obvious which method is more effective if a listener listens to the enhanced speech in noisy environments, a situation that often happens in silent speech communication. In this paper, assuming a typical situation in which NAM is uttered by a speaker in a quiet environment and conveyed to a listener in noisy environments, we investigate what kinds of target speech are more effective for NAM enhancement. We also propose NAM enhancement methods for converting NAM to other types of target voiced speech. Experiments show that the conversion process into voiced speech is more effective than that into unvoiced speech for generating more intelligible speech in noisy environments

Ultra-high-speed imaging of bubbles interacting with cells and tissue

Ultrasound contrast microbubbles are exploited in molecular imaging, where bubbles are directed to target cells and where their high-scattering cross section to ultrasound allows for the detection of pathologies at a molecular level. In therapeutic applications vibrating bubbles close to cells may alter the permeability of cell membranes, and these systems are therefore highly interesting for drug and gene delivery applications using ultrasound. In a more extreme regime bubbles are driven through shock waves to sonoporate or kill cells through intense stresses or jets following inertial bubble collapse. Here, we elucidate some of the underlying mechanisms using the 25-Mfps camera Brandaris128, resolving the bubble dynamics and its interactions with cells. We quantify acoustic microstreaming around oscillating bubbles close to rigid walls and evaluate the shear stresses on nonadherent cells. In a study on the fluid dynamical interaction of cavitation bubbles with adherent cells, we find that the nonspherical collapse of bubbles is responsible for cell detachment. We also visualized the dynamics of vibrating microbubbles in contact with endothelial cells followed by fluorescent imaging of the transport of propidium iodide, used as a membrane integrity probe, into these cells showing a direct correlation between cell deformation and cell membrane permeability