Children in an Urban Tanzania

One in four children being born in today‟s Tanzania is likely to be growing up in an urban area. It is projected to be one in three in the short time span of one generation. Tanzania is more urban than it perceives itself and official figures disclose. Urban Tanzanians feel emotionally rooted in their villages of origin rather than in the cities and towns where one quarter of the total population lives. Urbanisation figures fail to account for extensive high density areas just because they are not officially classified as urban. Despite a persisting rural self-representation, Tanzania is one of the fastest urbanising countries in one of the world‟s fastest urbanising regions. The nearly half urban population aged 0-18 may well be the first truly urbanised generation in the history of the nation.\ud As urbanisation is rapidly transforming the physical, social and economic landscape of the country, how has Tanzania equipped itself to provide adequate water, sanitation, health care, education, protection services to meet the fundamental needs and rights of a swelling number of urban children and communities? National policy and programmatic frameworks still broadly target rural poverty, perceived as the nation‟s core development challenge. Urban poverty, growing alongside urban affluence, remains mainly unaccounted for and, as a result, unaddressed. The condition of poor and marginalised urban groups escapes official urban figures. Standard urban-rural disaggregation generates statistical averages that overshadow sub-municipal disparities. Also poverty lines tend to underestimate actual poverty. Based on mere consumption levels, they disregard living conditions, thus leaving unaccounted for several necessities that poor households are normally forced to acquire through cash purchases in a monetised urban economy. As a result, urban poverty is broadly overlooked and poor urban children, lost in skewed official estimates and tucked away in peripheral unplanned urban fringes, risk remaining invisible in development policy and investments. In-depth analysis based on sub-municipal data is urgently needed to accurately measure urban poverty in its multiple dimensions of income poverty, inadequate access to basic services and powerlessness.\ud The assumption underpinning the limited attention that has been paid to urban poverty is that of an urban advantage. Undoubtedly, cities enjoy an edge over rural areas. Urbanisation drives the development of a whole nation. High population concentration, economies of scale, proximity and agglomeration make cities engines of growth. They offer greater avenues for livelihood and education, and should be expected to afford children better opportunities for survival, growth and development than rural areas. Better economic resources and political visibility hold a potential to offer higher incomes and enhance the scope for the government and the private sector to fund services and infrastructure. Density, favouring economies of scale, promises to favour delivering of essential services.\ud Children, adolescents and youth are attracted to city life, aspiring to access better jobs, higher education and a richer cultural life. Urban areas are also hubs of technological innovation, social exchange and mass communication. Urban children can draw from resources that are denied to rural peers.\ud The urban advantage, however, is being eroded. Provision of social services and infrastructure is failing to keep pace with growing demand being generated by urbanisation.\ud  Availability of basic services, expected to be markedly higher in urban centres as compared to remote rural areas, has been declining. Decreasing urban access to improved sources of drinking water over the past decade epitomises this trend. The traditional urban – rural social sector performance gap has been narrowing against most indicators in the areas of education, health, nutrition, water and sanitation. In some cases gaps have been actually bridged and rural areas are even outperforming urban centres.\ud 7\ud  As urban social sector performance is declining, it is likely that it is the poor, underserviced communities to remain unreached. Although statistical averages prevent any level of sub-municipal analysis, limited data available on access to basic services and health and education outcomes in low-income urban communities suggests that the urban poor may be faring even worse than their rural counterparts.\ud  Urbanisation growth is projected to continue in the future. If the present scenarios are not going to be addressed now, they are likely to deteriorate further. As density increases and unplanned settlements become more congested, investments in social facilities and infrastructure can only be expected to become costlier, both financially and socially.\ud If not properly leveraged, the potential advantage that cities offer can turn into a disadvantage. A concentration of children in areas where services and infrastructure are lacking is a major disadvantage. Children residing in overcrowded and degraded settlements characterised by poorly managed sanitation systems, inadequate provision of safe water, inefficient solid waste management are faced with one of the most life-threatening environments possible – with climate change posed to increase vulnerability further. Such a disadvantage can be daunting in a situation where the overwhelming majority of urban dwellers reside in unplanned settlements, which in Tanzania‟s primate city, Dar es Salaam, are estimated to accommodate over 80 percent of the population, one of the highest proportions in Sub-Saharan Africa.\ud Availability and access are not synonymous. In most cities, availability of basic services does not translate necessarily into access. Higher quality and availability of services needs to be equally distributed across social classes and space to achieve equal access by all citizens. The difference between successfully exploiting the urban advantage and passively reeling under the urban disadvantage can be made by the way access to resources is managed. A competent, accountable and equitable system of local governance can make that difference. Good local governance can help overcome the disparities that still bar access by the poor to safe water and sanitation, quality education, adequate health care and nutrition, affordable transport, secure land tenure and decent housing. Accountable local authorities, proactive communities and enabled children are the key actors in a local governance process leading to the creation of cities friendly to children.\ud Young people are already participating in local governance processes. They are active in children‟s municipal councils, children‟s school councils and other similar institutions. Avenues for child participation needs to be strengthened and opened to all children, not only in institutional settings, but also in families and communities having primary responsibility for children‟s well being. Cities and communities provide the most relevant scale for genuine children‟s participation, where young people can effectively engage in addressing the problems that directly affect them.\ud Though universal human rights and global development goals are set at the international and national levels, it is ultimately in a myriad of local Tanzanian communities that they are expected to be fulfilled – in the family, the school, the ward and ultimately the city. The city government offers an ideal platform for converging a plethora of sectoral interventions independently targeting children and delivering them holistically, at the local level where children live. The horizon of children is local. Within the local dimension, children‟s goals and rights can be met and monitored by duty bearers who have primary responsibility for their fulfilment. If development goals and human rights are not implemented locally, they are likely to remain abstract declarations of intent and sterile. Local authorities, communities, families and children together can transform today‟s child unfriendly urban settings into child-friendly cities – as cities friendly to children are friendly to all

Audit of Antenatal Testing of Sexually Transmissible Infections and Blood Borne Viruses at Western Australian Hospitals

In August 2007, the Western Australian Department of Health (DOH) released updated recommendations for testing of sexually transmissible infections (STI) and blood-borne viruses (BBV) in antenates. Prior to this, the Royal Australian & New Zealand College of Obstetricians & Gynaecologists (RANZCOG) antenatal testing recommendations had been accepted practice in most antenatal settings. The RANZCOG recommends that testing for HIV, syphilis, hepatitis B and C be offered at the first antenatal visit. The DOH recommends that in addition, chlamydia testing be offered. We conducted a baseline audit of antenatal STI/BBV testing in women who delivered at selected public hospitals before the DOH recommendations. We examined the medical records of 200 women who had delivered before 1st July 2007 from each of the sevenWAhospitals included in the audit. STI and BBV testing information and demographic data were collected. Of the 1,409 women included, 1,205 (86%) were non-Aboriginal and 200 (14%) were Aboriginal. High proportions of women had been tested for HIV (76%), syphilis (86%), hepatitis C (87%) and hepatitis B (88%). Overall, 72% of women had undergone STI/BBV testing in accordance with RANZCOG recommendations. However, chlamydia testing was evident in only 18% of records. STI/BBV prevalence ranged from 3.9% (CI 1.5– 6.3%) for chlamydia, to 1.7% (CI 1–2.4%) for hepatitis C, 0.7% (CI 0.3–1.2) for hepatitis B and 0.6% (CI 0.2–1) for syphilis. Prior to the DOH recommendations, nearly three-quarters of antenates had undergone STI/BBV testing in accordance with RANZCOG recommendations, but less than one fifth had been tested for chlamydia. The DOH recommendations will be further promoted with the assistance of hospitals and other stakeholders. A future audit will be conducted to determine the proportion of women tested according to the DOH recommendations. The hand book from this conference is available for download Published in 2008 by the Australasian Society for HIV Medicine Inc © Australasian Society for HIV Medicine Inc 2008 ISBN: 978-1-920773-59-

The Hidden Though Flourishing Justification of Intellectual Property Laws: Distributive Justice, National Versus International Approaches

Bu çalışmada, Gökkuşağı alabalıklarında enfeksiyonoluşturan Ichthyophthirius multifiliis’infarklı bölgelerden izole edilmiş saha suşlarının genotipik yapıları temelindeimmundominant özellik gösteren rekombinant immobilizan antijenlerini(i-antijen) kodlayan genlerin bakteriyel ekspresyon sistemine klonlanarakeksprese ve karakterize edilmeleri, antijenik profillerinin ortaya çıkarılmasıve aşı adayı olabilecek rekombinant antijenlerin elde edilmesi amaçlanmıştır.&nbsp;Çalışmada 2018 ve 2019 yıllarının Temmuz ve Ağustos ayları arasında Gökkuşağıalabalığı yetiştiriciliğinin yoğun yapıldığı Samsun, Rize, Kayseri, Elazığ,Burdur, Antalya ve Muğla illerinde bulunan işletmeler ziyaret edilerek balıkpopülasyonları üzerinde saha araştırmaları yürütülmüş ve I. multifiliis ile enfekte bulunan balıklardan ilgili protokolleregöre izolasyon gerçekleştirilmiştir. Laboratuvara uygun solüsyonlar ve soğukzincir altında intikal ettirilen örneklerden cDNA ve gDNA izolasyonlarıgerçekleştirilmiştir. I. multifiliis i-antijengen lokusunun amplifikasyonu amacıyla optimum primer dizaynı için ön çalışmalaryürütülmüş ve hedef gen bölgeleri uygun amplifikasyon koşullarında PCR’daçoğaltılmıştır. Elde edilen amplikonlar agaroz jel üzerinden saflaştırılmıştır.Multiple gen lokusu sekanslarının belirlenebilmesi amacıyla ilgili pürifiyeamplikonlar pJET 1.2 plazmit vektörüne CloneJET PCR cloning kit (Thermo FisherScientific) kullanılarak klonlanmış ve katı besi yerinde belirlenenkolonilerden rekombinant plazmid DNA izolasyonları gerçekleştirilmiştir. Rekombinantplazmidler spesifik primerlerle çift yönlü olarak sekanslanmış vekromotogramlar De Novo Assemble üzerinden işlenerek hedef insert sekanslarvektör plazmid DNA’sı içerisinden çıkarılmış ve konsensüs sekanslar eldeedilmiştir. İlgili primerlerin i-antijen gen lokusu içerisinde çoğalttığı fragmentlerinbelirlenebilmesi amacıyla PCR ürünleri ayrıca yeni nesil dizileme teknolojisi (NGS)kullanılarak işlenmiş ve elde edilen dizilimlerin gen veri tabanlarındakimevcut tüm i-antijen gen lokusları ile filogenetik ilişkileri araştırılmıştır.Tüm bu araştırmalar sonucu karakterize edilen i-antijen genlerinin ekspreseettiği proteinlerin rekombinant olarak eldesi için çalışmalargerçekleştirilmiştir. Aşı adayı potansiyeli olabilecek bazı lokusların bakteriyelekspresyon sistemine aktarımı için kodon optimizasyonları yapılarak pET-32a(+)ekspresyon plazmid DNA’sına (Novagen) klonlanması gerçekleştirilmiştir. Eldeedilen rekombinant plazmitler E. colikompotent BL21(DE3) hücrelerine transforme edilerek optimum koşullardaekspresyon çalışmaları yürütülmüş ve ekspresyon etkinliği SDS-PAGE ve WesternBlot analizleri ile belirlenmiştir. Eksprese edilen rekombinant i-antijenproteinleri afinite kromotografi kulanılarak saflaştırılmış ve immunreaktiflikleri Western Blot analizleri ile tespit edilmiştir.&nbsp;Çalışmada,Elazığ, Rize ve Muğla illerinde ziyaret edilen işletmelerdeki Gökkuşağıalabalıklarında deri ve solungaçlarından hazırlanan preparatların mikroskobikincelemeleri ile I. multifiliisenfeksiyonunun yaygın olduğu görülmüştür. İlgili bölgelerden elde edilen I. multifiliis suşlarına ait cDNA vegDNA izolatlarının i-antijen gen lokusunun dizayn edilen pirmerlerle PCR’daamplifiye edilmesi sonucu 1200-1300 bp büyüklüğünde amplikonlar saptanmıştır.Pürifiye amplikonların klonlanması sonucu ilgili izolatlara ait açık okumaçerçevesi (ORF) sekanslarının analizinde birbirleriyle %38,3-58,8 arasındafarklılık gösteren 4 farklı i-antijen izoformu tespit edilmiştir. Bu izoformlararasında bir antijenik lokusun (ImulTR1-iant) her üç ildeki alabalıkpopülasyonlarından izole edilen I.multifiliis suşlarında da var olduğu NGS analizlerinde görülmüştür.ImulTR1-iant ORF sekansı ayrıca Amerika Birleşik Devletleri’nde alabalıklardanizole edilmiş bir i-antijen izoformuyla %83,3 identiklik gösterirken, diğertespit edilen izoformlara ait sekansların GenBank veri tabanında mevcuti-antijen sekanslarından oldukça farklı olduğu belirlenmiştir. Araştırmadarekaombinant i-antijen eldesi için yaygın belirlenen ImulTR1-iant izolatıüzerinden analizler yürütülmüştür. Bu izoformu kodlayan gen bölgesinin 1263bpbüyüklüğünde olduğu ve ORF’nin 420 amino asitten teşekkül ettiğibelirlenmiştir. ORF amino asit sekanslarının in-slico analizlerde 42,552 kDabüyüklüğünde bir proteini eksprese ettiği, bu proteinin sitoplazmik olduğu vetransmembran bölge içermediği tespit edilmiştir. Ayrıca ilgili ORF sekansıiçerisinde uzunluğu 7-22aa arasında değişen 21 antijenik bölge olduğubelirlenmiştir. Kodon optimizasyonu yapılmış olan ImulTR1-iant izoformuna ait pET-32a(+)rekombinant plazmitinin E. colikompotent BL21(DE3) hücrelerine transformasyonu ve ekspresyonu sonrasındayapılan SDS-PAGE analizlerinde in-silico analiz sonuçlarına paralel olarakyaklaşık 43kDa’luk protein jel üzerinde görüntülenmiştir. İlgili rekombinantprotein afinite kromotografide HisTrap FF crude (GE Healthcare) kolonlarıkullanılarak saflaştırılmış ve pürifiye rekombinant antijenin immun-reaktifliğiWestern-Blot analizleriyle gösterilmiştir.&nbsp;ErciyesÜniversitesi Bilimsel Araştırma Projeleri Birimi tarafından TOA-2017-7742 kodnumarasıyla desteklenen bu çalışma ile Türkiye’de gökkuşağı alabalıklarındasorun oluşturan ve ekonomik kayıplara yol açan I. multifiliis suşlarına karşı biyoteknolojik aşı geliştirilmesinoktasında aşı adayı olabilecek immobilizan antijenler üzerine özgün veriler sağlanmıştır.Elde edilen aşı adayı rekombinant antijenlerin etkinliğini ortaya koymanoktasında laboratuvar ve saha şartlarında immunizasyon ve çelınç enfeksiyondenemeleri için yeni proje çalışmaları planlanmaktadır.</style

Ending Child Marriage in a Generation: What Research is Needed?

In recent years, the obstacles that child marriage poses to development and to the achievement of the Millennium Development Goals have been widely recognized. Varied responses to the problem have been devised. The number of programs testing different approaches to ending child marriage has grown, and many have been evaluated. We are starting to get a sense of what works and the general areas in which investing in research could make a difference.The purpose of this paper is to identify gaps in the research on child marriage in which additional investment could catalyze change. Much remains to understand about child marriage and how to influence it. By mapping out current knowledge of child marriage and the programs designed to address it, and by highlighting questions to which we do not yet know the answers, the paper is intended to generate discussion in the field and clarify what we need to know to bring an end to this deeply harmful practice.The paper also moves us closer to the development of a shared theory of change for this field: given that many organizations are now working in this area, how can their programs and related research be understood -- aligned -- in such a way that their work feeds into a single theory of change?The research gaps on their own should not drive investments in child marriage. A worthy research mandate must also be driven by programmatic and advocacy needs. The recommended areas for research are not meant to provide a definitive menu, but rather to describe the general contours of what we know and what we need to understand better

Lifeworld Analysis

We argue that the analysis of agent/environment interactions should be extended to include the conventions and invariants maintained by agents throughout their activity. We refer to this thicker notion of environment as a lifeworld and present a partial set of formal tools for describing structures of lifeworlds and the ways in which they computationally simplify activity. As one specific example, we apply the tools to the analysis of the Toast system and show how versions of the system with very different control structures in fact implement a common control structure together with different conventions for encoding task state in the positions or states of objects in the environment.Comment: See http://www.jair.org/ for any accompanying file