Light control of the flow of phototactic microswimmer suspensions

Some micro-algae are sensitive to light intensity gradients. This property is known as phototaxis: the algae swim toward a light source (positive phototaxis). We use this property to control the motion of micro-algae within a Poiseuille flow using light. The combination of flow vorticity and phototaxis results in a concentration of algae around the center of the flow. Intermittent light exposure allows analysis of the dynamics of this phenomenon and its reversibility. With this phenomenon, we hope to pave the way toward new algae concentration techniques (a bottleneck challenge in hydrogen algal production) and toward the improvement of pollutant bio-detector technology

Calculating the global contribution of coralline algae to carbon burial

The ongoing increase in anthropogenic carbon dioxide (CO2) emissions is changing the global marine environment and is causing warming and acidification of the oceans. Reduction of CO2 to a sustainable level is required to avoid further marine change. Many studies investigate the potential of marine carbon sinks (e.g. seagrass) to mitigate anthropogenic emissions, however, information on storage by coralline algae and the beds they create is scant. Calcifying photosynthetic organisms, including coralline algae, can act as a CO2 sink via photosynthesis and CaCO3 dissolution and act as a CO2 source during respiration and CaCO3 production on short-term time scales. Long-term carbon storage potential might come from the accumulation of coralline algae deposits over geological time scales. Here, the carbon storage potential of coralline algae is assessed using meta-analysis of their global organic and inorganic carbon production and the processes involved in this metabolism. Organic and inorganic production were estimated at 330 g C m−2 yr−1 and 880 g CaCO3 m−2 yr−1 respectively giving global organic/inorganic C production of 0.7/1.8 × 109 t C yr−1. Calcium carbonate production by free-living/crustose coralline algae (CCA) corresponded to a sediment accretion of 70/450 mm kyr−1. Using this potential carbon storage by coralline algae, the global production of free-living algae/CCA was 0.4/1.2 × 109 t C yr−1 suggesting a total potential carbon sink of 1.6 × 109 t C yr−1. Coralline algae therefore have production rates similar to mangroves, saltmarshes and seagrasses representing an as yet unquantified but significant carbon store, however, further empirical investigations are needed to determine the dynamics and stability of that store

Effects of pharmaceutical wastes on growth of microalgae

The purpose of this work was to assay samples of waste material from Puerto Rican pharmaceutical industries for inhibition of growth of algae. Two samples (noted as I and II) supplied to us were tested for toxicity to six microalgae. The test organisms, two blue-green algae, two green algae, and two diatoms [r]epresent three major divisions of algae.University of Texas Marine Science InstituteMarine Scienc

Current US algae biorefineries, projects and perspectives

Large challenges remain to take advantage of algae (cyanobacteria, microalgae, and macroalgae) as a bioenergy, biofuels, and bioproducts crop. Traditional applications in the nutraceutical and food industry, have shown more promise. New efforts in combining a biorefinery approach with wastewater treatment, anaerobic digestion, and biomass utilization are becoming more popular and successful towards providing a middle ground for implementation of algae cultivation systems. An overview will be provided of the growth in fundamental and applied algae research in recent years, which will include a review of the scientific literature and key programs in bioenergy in the US leading to a resurgence and interest in algae. With this presentation, a discussion will ensue on the challenges being tackled to make the algae biorefinery a viable industry, including progress and opportunities available to develop algae as a key crop for the bioenergy and the bioproducts industries.Universidad de Málaga.Campus de Excelencia Internacional Andalucia Tec

SAMSON: Spectral Absorption-fluorescence Microscopy System for ON-site-imaging of algae

This paper presents SAMSON, a Spectral Absorption-fluorescence Microscopy System for ON-site-imaging of algae within a water sample. Designed to be portable and low-cost for on-site use, the optical sub-system of SAMSON consists of a mixture of low-cost optics and electronics, designed specifically to capture both fluorescent and absorption responses from a water sample. The graphical user interface (GUI) sub-system of SAMSON was designed to enable flexible visualisation of algae in the water sample in real-time, with the ability to perform fine-grained exposure control and illumination wavelength selection. We demonstrate SAMSON's capabilities by equipping the system with two fluorescent illumination sources and seven absorption illumination sources to enable the capture of multispectral data from six different algae species (three from the Cyanophyta phylum (blue-green algae) and three from the Chlorophyta phylum (green algae)). The key benefit of SAMSON is the ability to perform rapid acquisition of fluorescence and absorption data at different wavelengths and magnification levels, thus opening the door for machine learning methods to automatically identify and enumerate different algae in water samples using this rich wealth of data

Lipid content and biomass analysis in autotrophic and heterotrophic algal species

Biofuels are a form of renewable energy derived from living matter, typically plants. The push for biofuels began in order to decrease the amount of carbon dioxide (CO2) released into the atmosphere, as biofuels are essentially carbon neutral. The idea is the same amount of CO2 the plants took in to perform photosynthesis will then be released in the burning of the biofuels. Algae is an excellent source of biofuels because it grows quickly and is versatile in terms of the type of fuel it can produce. The two most common mechanisms for algae growth are heterotrophic or photoautotrophic. Heterotrophically grown algae uses an exogenous energy source, such as glucose, and uses the energy stored in it to perform cellular functions. Glucose also serves as a source of carbon and hydrogen, which are the primary elements found in lipids. In addition heterotrophic algae requires other nutrients for survival, such as water, vitamins, and inorganic ions. Algae grown photoautotrophically uses pigments in cellular photoreceptors to convert energy from light into adenosine triphosphate (ATP), an energy source, and to produce glucose. It also requires water, vitamins, and inorganic ions like the heterotrophic algae does. Some algal species, such as Chlorella zofingiensis, can be grown both photoautotrophically and heterotrophically. This algae species will be the subject of our experiment. Our experiment seeks to discover the most efficient way of growing algae to produce the highest amount of lipids. In addition to serving as a key component of cell and organelle membranes, lipids are a common form of high efficiency, long-term energy storage for living organisms, which is why lipids are extracted and processed to form biofuels. We propose growing one species of algae photoautotrophically by providing it with proper amounts of light but eliminating any glucose available. We will also grow the same species heterotrophically, with exogenous access to glucose, but eliminating all exposure to light sources. Finally, we will grow the same species mixotrophically with access to both glucose and light. Once the algae is grown, it will be harvested and analyzed for its lipid profile to determine which algae sample has the highest percent lipid content. We will also measure the percent biomass of each sample to determine which primary energy source leads to the greatest amount of total algal growth, percent organic material, and percent lipid content. We predict the algae grown with access to both sunlight and exogenous glucose will produce both the highest lipid content and the highest percent of biomass

Phylogenetic Analysis of Algal Symbionts Associated with Four North American Amphibian Egg Masses

Egg masses of the yellow-spotted salamander Ambystoma maculatum form an association with the green alga “Oophila amblystomatis” (Lambert ex Wille), which, in addition to growing within individual egg capsules, has recently been reported to invade embryonic tissues and cells. The binomial O. amblystomatis refers to the algae that occur in A. maculatum egg capsules, but it is unknown whether this population of symbionts constitutes one or several different algal taxa. Moreover, it is unknown whether egg masses across the geographic range of A. maculatum, or other amphibians, associate with one or multiple algal taxa. To address these questions, we conducted a phylogeographic study of algae sampled from egg capsules of A. maculatum, its allopatric congener A. gracile, and two frogs: Lithobates sylvatica and L. aurora. All of these North American amphibians form associations with algae in their egg capsules. We sampled algae from egg capsules of these four amphibians from localities across North America, established representative algal cultures, and amplified and sequenced a region of 18S rDNA for phylogenetic analysis. Our combined analysis shows that symbiotic algae found in egg masses of four North American amphibians are closely related to each other, and form a well-supported clade that also contains three strains of free-living chlamydomonads. We designate this group as the ‘Oophila’ clade, within which the symbiotic algae are further divided into four distinct subclades. Phylogenies of the host amphibians and their algal symbionts are only partially congruent, suggesting that host-switching and co-speciation both play roles in their associations. We also established conditions for isolating and rearing algal symbionts from amphibian egg capsules, which should facilitate further study of these egg mass specialist algae

A Mathematical model to guide Genetic Engineering of Photosynthetic Metabolism

open5noThe optimization of algae biomass productivity in industrial cultivation systems requires genetic improvement of wild type strains isolated from nature. One of the main factors affecting algae productivity is their efficiency in converting light into chemical energy and this has been a major target of recent genetic efforts. However, photosynthetic productivity in algae cultures depends on many environmental parameters, making the identification of advantageous genotypes complex and the achievement of concrete improvements slow. In this work, we developed a mathematical model to describe the key factors influencing algae photosynthetic productivity in a photobioreactor, using experimental measurements for the WT strain of Nannochloropsis gaditana. The model was then exploited to predict the effect of potential genetic modifications on algae performances in an industrial context, showing the ability to predict the productivity of mutants with specific photosynthetic phenotypes. These results show that a quantitative model can be exploited to identify the genetic modifications with the highest impact on productivity taking into full account the complex influence of environmental conditions, efficiently guiding engineering efforts.embargoed_20181201Perin, Giorgio; Bernardi, Andrea; Bellan, Alessandra; Bezzo, Fabrizio; Morosinotto, TomasPerin, Giorgio; Bernardi, Andrea; Bellan, Alessandra; Bezzo, Fabrizio; Morosinotto, Toma

Algae Biofuel Triacylglyceride Transesterification Optimization

Algae biofuels may hold the key to solving the problem of fossil fuel consumption by being comparable in content, renewable, and carbon-neutral. Many biofuel researchers and corporations have undertaken to increase the production rate or capacity of triacylglycerides (TAG), the fat precursor to biodiesel fuel produced by algae, in algae cultures and published articles documenting their findings. This research is devoted to evaluating the effect of water that may be present in samples on the conversion efficiency of TAG into fatty acid methyl esters (FAME), commonly referred to as biodiesel. Therefore, that efficiency was studied to find the water content which optimizes the yield and determine if further drying of algae was necessary as an additional step in sample preparation. The results showed that the water content typically present in lyophilized algae samples is not sufficient to appreciably inhibit the reaction efficiency and necessitate extensive drying as a sample preparation step prior to transesterification