The ecological dichotomy of ammonia-oxidizing archaea and bacteria in the hyper-arid soils of the Antarctic Dry Valleys

The McMurdo Dry Valleys of Antarctica are considered to be one of the most physically and chemically extreme terrestrial environments on the Earth. However, little is known about the organisms involved in nitrogen transformations in these environments. In this study, we investigated the diversity and abundance of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in four McMurdo Dry Valleys with highly variable soil geochemical properties and climatic conditions: Miers Valley, Upper Wright Valley, Beacon Valley and Battleship Promontory. The bacterial communities of these four Dry Valleys have been examined previously, and the results suggested that the extremely localized bacterial diversities are likely driven by the disparate physicochemical conditions associated with these locations. Here we showed that AOB and AOA amoA gene diversity was generally low; only four AOA and three AOB operational taxonomic units (OTUs) were identified from a total of 420 AOA and AOB amoA clones. Quantitative PCR analysis of amoA genes revealed clear differences in the relative abundances of AOA and AOB amoA genes among samples from the four dry valleys. Although AOB amoA gene dominated the ammonia-oxidizing community in soils from Miers Valley and Battleship Promontory, AOA amoA gene were more abundant in samples from Upper Wright and Beacon Valleys, where the environmental conditions are considerably harsher (e.g., extremely low soil C/N ratios and much higher soil electrical conductivity). Correlations between environmental variables and amoA genes copy numbers, as examined by redundancy analysis (RDA), revealed that higher AOA/AOB ratios were closely related to soils with high salts and Cu contents and low pH. Our findings hint at a dichotomized distribution of AOA and AOB within the Dry Valleys, potentially driven by environmental constraints

Distribution and Diversity of Archaeal and Bacterial Ammonia Oxidizers in Salt Marsh Sediments

Diversity and abundance of ammonia-oxidizing Betaproteobacteria (β-AOB) and archaea (AOA) were investigated in a New England salt marsh at sites dominated by short or tall Spartina alterniflora (SAS and SAT sites, respectively) or Spartina patens (SP site). AOA amoA gene richness was higher than β-AOB amoA richness at SAT and SP, but AOA and β-AOB richness were similar at SAS. β-AOB amoA clone libraries were composed exclusively of Nitrosospira-like amoA genes. AOA amoA genes at SAT and SP were equally distributed between the water column/sediment and soil/sediment clades, while AOA amoA sequences at SAS were primarily affiliated with the water column/sediment clade. At all three site types, AOA were always more abundant than β-AOB based on quantitative PCR of amoA genes. At some sites, we detected 109 AOA amoA gene copies g of sediment−1. Ratios of AOA to β-AOB varied over 2 orders of magnitude among sites and sampling dates. Nevertheless, abundances of AOA and β-AOB amoA genes were highly correlated. Abundance of 16S rRNA genes affiliated with Nitrosopumilus maritimus, Crenarchaeota group I.1b, and pSL12 were positively correlated with AOA amoA abundance, but ratios of amoA to 16S rRNA genes varied among sites. We also observed a significant effect of pH on AOA abundance and a significant salinity effect on both AOA and β-ΑΟΒ abundance. Our results expand the distribution of AOA to salt marshes, and the high numbers of AOA at some sites suggest that salt marsh sediments serve as an important habitat for AOA

Word frequency affects naming latency in Dutch when age of acquisition is controlled.

Morrison and Ellis (1995) claim that most evidence of frequency effects in word recognition is not genuine but an artefact of the age at which the words have been acquired. The finding that age of acquisition (AOA) has a reliable independent effect on word naming is replicated for the Dutch language. However, it is also shown that the effect of word frequency remains reliable with AOA controlled. A possible interpretation is that the English studies have been based on retrospective student ratings, whereas in the present study a more on-line measure of AOA was used

Research on 2×2 MIMO Channel with Truncated Laplacian Azimuth Power Spectrum

Multiple-input multiple-output (MIMO) Rayleigh fading channel with truncated Laplacian azimuth power spectrum (APS) is studied. By using the power correlation matrix of MIMO channel model and the modified Jakes simulator, into which with random phases are inserted, the effect of the azimuth spread (AS), angle of departure (AOD) and angle of arrival (AOA) on the spatial correlation coefficient and channel capacity are investigated. Numerical results show that larger AS generates smaller spatial correlation coefficient amplitude, while larger average AOD or AOA produces larger spatial correlation coefficient amplitude. The average capacity variation is comprehensively dominated by the average AOD, AOA and AS

Optimal Precoders for Tracking the AoD and AoA of a mm-Wave Path

In millimeter-wave channels, most of the received energy is carried by a few paths. Traditional precoders sweep the angle-of-departure (AoD) and angle-of-arrival (AoA) space with directional precoders to identify directions with largest power. Such precoders are heuristic and lead to sub-optimal AoD/AoA estimation. We derive optimal precoders, minimizing the Cram\'{e}r-Rao bound (CRB) of the AoD/AoA, assuming a fully digital architecture at the transmitter and spatial filtering of a single path. The precoders are found by solving a suitable convex optimization problem. We demonstrate that the accuracy can be improved by at least a factor of two over traditional precoders, and show that there is an optimal number of distinct precoders beyond which the CRB does not improve.Comment: Resubmission to IEEE Trans. on Signal Processing. 12 pages and 9 figure

An eye movement corpus study of the age-of-acquisition effect

In the present study, we investigated the effects of word-level age of acquisition (AoA) on natural reading. Previous studies, using multiple language modalities, showed that earlier-learned words are recognized, read, spoken, and responded to faster than words learned later in life. Until now, in visual word recognition the experimental materials were limited to single-word or sentence studies. We analyzed the data of the Ghent Eye-tracking Corpus (GECO; Cop, Dirix, Drieghe, & Duyck, in press), an eyetracking corpus of participants reading an entire novel, resulting in the first eye movement megastudy of AoA effects in natural reading. We found that the ages at which specific words were learned indeed influenced reading times, above other important (correlated) lexical variables, such as word frequency and length. Shorter fixations for earlier-learned words were consistently found throughout the reading process, in both early (single-fixation durations, first-fixation durations, gaze durations) and late (total reading times) measures. Implications for theoretical accounts of AoA effects and eye movements are discussed

Multi-Array 5G V2V Relative Positioning: Performance Bounds

We study the performance bounds of vehicle-to-vehicle (V2V) relative positioning for vehicles with multiple antenna arrays. The Cram\'{e}r-Rao bound for the estimation of the relative position and the orientation of the Tx vehicle is derived, when angle of arrival (AOA) measurements with or without time-difference of arrival (TDOA) measurements are used. In addition, geometrically intuitive expressions for the corresponding Fisher information are provided. The derived bounds are numerically evaluated for different carrier frequencies, bandwidths and array configurations under different V2V scenarios, i.e. overtaking and platooning. The significance of the AOA and TDOA measurements for position estimation is investigated. The achievable positioning accuracy is then compared with the present requirements of the 3rd Generation Partnership Project (3GPP) 5G New Radio (NR) vehicle-to-everything (V2X) standardization

Dissociating word frequency and age of acquisition: The Klein effect revived (and reversed).

The Klein effect (G. S. Klein, 1964) refers to the finding that high-frequency words produce greater interference in a color-naming task than low-frequency words. The present study used the Klein effect to investigate the relationship between frequency and age of acquisition (AoA) by measuring their influence on color naming. Two experiments showed reliable effects of frequency (though in the opposite direction to that reported by Klein) but no effects of AoA. Experiment 1 produced a dissociation between frequency and AoA when manipulated orthogonally. Experiment 2 produced the same dissociation using different stimuli. In contrast, both variables reliably influenced word naming. These findings are inconsistent with the view that frequency and AoA are 2 aspects of a single underlying mechanism