A Multiplexed Biosensor Based on Biomolecular

Abstract—In this paper we describe the fabrication and characterization of a multiplexed biosensor based on molecular bio-wires that can be used for detecting multiple pathogens in a biological sample. At the core of the proposed device is a biosensor that operates by converting binding events between antigen and antibody into a measurable electrical signal using polyaniline nanowires as transducers. By mixing and patterning antibodies at different spatial locations of the biosensor, the response of the biosensor can be configured to detect the presence of either one of several pathogens present in the analyte, thus making it ideal for rapid environment screening applications. Experiments using the biosensor array specific to B. cereus and E. coli bacteria validate the functionality of the proposed multiplexed architecture. Index Terms—Biosensors, polyaniline, multiplexed detection, environmental monitoring I