Motivation: EMAN is one of the most popular software packages for single particle reconstruction. But the particle clusters produced during its model refining stage are of low qualities. We attempt to refine the particle clusters by more accurately determining orientations of particles, and thereby achieving higher resolutions of consequent 3D structures

Fan, Liya

Liu, Zhiyong

Wang, Gongming

Zhang, Fa

PubMed Central

English

Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization.

Document clustering using an inverted ﬁle approach.

EMAN: semiautomated software for high-resolution singleparticle reconstructions.

IMIRS: a high-resolution 3D reconstruction package integrated with a relational image database.

Measures of resolution using Fourier shell correlation.

SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related ﬁelds.

The parallelization of SPIDER on distributed-memory computers using MPI.

A framework to refine particle clusters produced by EMAN