A Fully Automatic Alignment of Electron Tomography Images Without Fiducial Markers

In structural biology, electron tomography is used in reconstructing three-dimensional objects such as macromolecules, viruses, and cellural organelles to learn their structure and properties. In order to successfully perform the three-dimensional reconstruction from a series of transparent two-dimensional images, the images have to be aligned or registered. In this paper, we propose a multi-phase method where the registration process is fully automated without the need for fiducial markers such as gold particles. Our experiments show promising results.

A Fully Automatic Alignment of Electron Tomography Images Without Fiducial Markers

In structural biology, electron tomography is used in reconstructing three-dimensional objects such as macromolecules, viruses, and cellural organelles to learn their structure and properties. In order to successfully perform the three-dimensional reconstruction from a series of transparent two-dimensional images, the images have to be aligned or registered. In this paper, we propose a multi-phase method where the registration process is fully automated without the need for ducial markers such as gold particles. Our experiments show promising results. 1 Introduction Electron tomography means reconstructing the interior of an object from its electron microscope images. In order to perform the 3D reconstruction successfully, the motion between the successive images must be solved, i.e., the images have to be aligned or registered. In this paper we use a set of twodimensional electron microscope images of a threedimensional object which are obtained by tilting the microscope around one..