6 research outputs found

    AFM blocking assays with free PGM for the wild-type TIL448.

    No full text
    <p>(A, B) Histograms of adhesion forces and (C, D) typical force-distance curves for wild-type TIL448 lacto-probe in interaction with PGM-coated polystyrene, using AFM force spectroscopy in milliQ-grade water, before (A, C) and after (B, D) incubation with free PGM. One representative experiment (1024 force curves) is shown.</p

    Composition of the acidic fraction of O-glycans released from PGM.

    No full text
    <p>Monosaccharide composition of individual glycans was established by MALDI-TOF-MS based on the <i>m/z</i> values of monosaccharides.</p><p>Nature of monosaccharides (Gal, GlcNAc and Fuc) was based on GC composition analysis and on previous reports <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079850#pone.0079850-Karlsson1" target="_blank">[48]</a>. <i>m/z</i> values of sialylated glycans correspond to [M+Na]<sup>+</sup> adducts whereas sulphated glycans exhibited [M+2Na-H]<sup>+</sup> adducts.</p

    Composition of the neutral fraction of O-glycans released from PGM. Monosaccharide composition of individual glycans was established by MALDI-TOF-MS based on the <i>m/z</i> values of monosaccharides.

    No full text
    <p>Nature of monosaccharides (Gal, GlcNAc and Fuc) was based on GC composition analysis and on previous reports <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079850#pone.0079850-Karlsson1" target="_blank">[48]</a>. <i>m/z</i> values of neutral glycans correspond to [M+Na]<sup>+</sup> adducts.</p
    corecore