10 research outputs found

    Chromosome composition of the intergeneric BC<sub>2</sub> and BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.

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    <p><b>Note:</b> Since small variation of chromosome counts can occur due to the loss or the overlapping of a few chromosomes from the preparation, the modal number of chromosomes and the range of total numbers of chromosomes in 2n cell are presented for the sugarcane clones analyzed. S and E indicate <i>Saccharum</i> spp. chromosome and <i>E</i>. <i>arundinaceus</i> chromosome, respectively. S/E and E/S indicate <i>Saccharum</i> spp. centromere with <i>E</i>. <i>arundinaceus</i> chromosome segment and <i>E</i>. <i>arundinaceus</i> centromere with <i>Saccharum</i> spp. chromosome segment, respectively.</p><p>Chromosome composition of the intergeneric BC<sub>2</sub> and BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.</p

    The intergeneric BC<sub>2</sub> and BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.

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    <p><b>Note</b>: “YCE” series are the progeny of <i>E</i>. <i>arundinaceus</i>, the other plant materials are the commercial cultivars containing germplasm from <i>Saccharum</i> spp. without contribution from <i>E</i>. <i>arundinaceus</i>.</p><p>The intergeneric BC<sub>2</sub> and BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.</p

    GISH analysis of the intergeneric BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>. <i>Saccharum</i> spp. chromosomes are visualized in red and <i>E</i>. <i>arundinaceus</i> chromosomes in green.

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    <p>(A) YCE05-64: 2n = 118 = 107S + 6E + 2(E/S)+3(S/E); (B) YCE05-150: 2n = 116 = 108S + 8E; (C) YCE06-61: 2n = 114 = 107S + 7E; (D) YCE06-63: 2n = 105 = 98S + 7E; (E) YCE06-92: 2n = 118 = 109S + 7E + 2(E/S); (F) YCE06-111: 2n = 108 = 103S + 4E + E/S; (G) YCE06-140: 2n = 112 = 106S + 5E + S/E; (H) YCE06-166: 2n = 110 = 105S + 5E. The arrowheads in Fig 2A, Fig 2E, Fig 2F and Fig 2G show the translocated chromosome. S and E indicate <i>Saccharum</i> spp. chromosome and <i>E</i>. <i>arundinaceus</i> chromosome, respectively. S/E and E/S indicate <i>Saccharum</i> spp. centromere with <i>E</i>. <i>arundinaceus</i> chromosome segment and <i>E</i>. <i>arundinaceus</i> centromere with <i>Saccharum</i> spp. chromosome segment, respectively. Scale bars: 5 μm.</p

    The intergeneric BC<sub>2</sub> and BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.

    No full text
    <p><b>Note</b>: “YCE” series are the progeny of <i>E</i>. <i>arundinaceus</i>, the other plant materials are the commercial cultivars containing germplasm from <i>Saccharum</i> spp. without contribution from <i>E</i>. <i>arundinaceus</i>.</p><p>The intergeneric BC<sub>2</sub> and BC<sub>3</sub> progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.</p

    Analysis of nrDNA-ITS sequences.

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    <p>“······” represents sequences that are identical to that of Badila. Only base mutations are shown.</p

    Electrophoretogram of nrDNA-ITS PCR products.

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    <p>M: 100 bp DNA ladder marker. Lanes 1–5: Badila, VN cattle cane, Loethers, Crystalina, Cheribon, respectively, belong to <i>S</i>. <i>officinarum</i>. Lanes 6–10: 57NG208, 51NG63, NG77-004, 28NG21, Daye, respectively, belong to <i>S</i>. <i>robustum</i>. Lanes 11–20: YN75-2-11, YN82-110, YN83-160, FJ89-1-1, YN83-201, YN82-44, YN83-171, GZ78-2-28, FJ88-1-13, FJ89-1-19, respectively, belong to <i>S</i>. <i>spontaneum</i>.</p

    Electrophoretogram of tetra-primer ARMS PCR products.

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    <p>M: 100 bp DNA ladder marker. Lanes 1–5: Badila, VN cattle cane, Loethers, Crystalina, and S. Cheribon, respectively, are <i>S</i>. <i>officinarum</i>. Lanes 6–8: Muckche, Baimeizhe, and Canablanca, respectively, are F<sub>1</sub> generations produced between <i>S</i>. <i>officinarum</i> and <i>S</i>. <i>spontaneum</i>. Lanes 9–14: 28NG21, 51NG63, 51NG3, Daye, 57NG208, and NG77-004, respectively, are <i>S</i>. <i>robustum</i>. Lanes 15–39: YN75-2-11, YN83-160, YN83-225, YN82-58, YN4, YN83-238, Vietnam-3, YN82-9, YN-mengzi, YN84-268, YN82-110, GZ78-1-11, YN76-1-16, FJ89-1-11, YN82-50, SC92-42, FJ89-1-1, YN83-201, SC88-49, FJ89-1-21, YN76-1-24, YN75-2-35, SC79-2-16, SC79-1-26, and YN83-171, respectively, are <i>S</i>. <i>spontaneum</i>. Lanes 40–42: Wenshan cane, Uba, and GD-sinense, respectively, are <i>S</i>. <i>sinense</i>. Lanes 43–45: Nagans, Pansahi, and Saretha, respectively, are <i>S</i>. <i>barberi</i>. 46–48: ROC10, ROC22, and CP84-1196, respectively, are cultivars. Lanes 49–66: FJ92-1-11, Heqing, YN75-2-35, GD-16, GD-60, GD-71, YN82-44, Ledong-1, Xundian, GZ78-1-5, GZ79-1-4, FJ88-1-13, GZ78-2-28, FJ-HuiAn, FJ89-1-16, GD-30, FJ89-1-18, and FJ89-1-19, respectively, are <i>S</i>. <i>spontaneum</i>. Lanes 67–71: RL12-38-1, RL12-38-5, RL12-38-76, RL12-38-81, and RL12-38-84, respectively, are F<sub>1</sub> generations produced between <i>S</i>. <i>officinarum</i> and <i>S</i>. <i>robustum</i>. Lane 72: H<sub>2</sub>O.</p
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