Genotoxic Evaluation and Bioindicator Potential of Myotis capaccinii in Serbia: Element Concentrations and DNA Damage as Pollution Markers

Abstract

Bats reflect environmental quality in diverse habitats, from cities to forests. This study aimed to reveal element bioaccumulation and DNA damage in the long-fingered bat (Myotis capaccinii) using non-lethal methods. Twenty-four hair samples and 15 blood samples were taken from bats caught at two locations in Eastern Serbia between July and September of 2024. Hair analysis by inductively coupled plasma–optical emission spectrometry (ICP-OES) detected As, Cr, Fe, Li, Mn, Ni, and Zn, while Cd, Co, Cu and, Pb were below detection limits. The degree of DNA damage in peripheral blood was determined using the alkaline comet assay. In M. capaccinii, there was a significant difference in element concentrations between sexes, with females having higher concentrations of Cr, Fe, and Mn, and between seasons, with samples from summer having higher concentrations of Ni and Mn. A significant difference in zinc concentration was observed between adults and juveniles. DNA damage was higher in the summer season. Significant correlations were confirmed between element concentrations in hair (Cr–Fe, Cr–Zn, Cr–Mn, Fe–Zn, and Fe–Mn) and between Cr and Zn concentrations and DNA damage in blood cells. The Cr–DNA damage link highlights hair as a marker for sustained environmental exposure. Every significant correlation that included Zn was negative. This is the first genotoxic study of bats in Serbia and of M. capaccinii globally, laying the groundwork for future research in other bat species and the wider Central Balkans. It marks an initial step toward using bats as bioindicators for noninvasive pollution monitoring.M213.