A Prevalence Study on Anoplocephala spp. in Serbian Horses: Navigating Diagnostic Challenges and Understanding Infection Risks

Abstract

Anoplocephala spp. are common equine tapeworm species in Europe, frequently found in grazing horses. Anoplocephala perfoliata is the most pathogenic, clinically significant species associated with gastrointestinal disorders, particularly colic, and can have a fatal outcome in some horses. The aim of this study was to determine the infection prevalence of Anoplocephala spp. in Serbia and to identify relevant risk factors. A total of 173 horses from various regions were tested using a combination of diagnostic methods: coprological analysis via combined sedimentation–flotation and the Mini-FLOTAC technique, as well as serological testing using a commercial ELISA test. The overall prevalence was 38.7%, with a higher number of positive cases being identified by serology. It was demonstrated that coprology and serology are complementary approaches for prevalence studies. Infection risk factors included high stocking density, free-roaming status and co-infection with nematodes, while lower rainfall and temperate climate were associated with reduced risk of tapeworm infection. These findings highlight the challenges of Anoplocephala spp. detection and provide insight into the epidemiology of equine tapeworm infection in Southeastern Europe