The interaction of matrix components and cells regulates the cellular and molecular process of glioma cells

Abstract

Presented to the 21st Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Rhatigan Student Center, Wichita State University, April 11, 2025.Research completed in the Department of Biological Sciences, Fairmount College of Liberal Arts and Sciences.INTRODUCTION: The production of fibrous proteins such as collagens in glioma extracellular matrix (ECM) increase compared with normal brain. The increased collagen type I and type IV alter the brain microenvironment and result in the change of glioma cell motility. Studies suggested that the increased collagen level in matrix altered the mechanical properties of the brain ECM and the increase of matrix stiffness may increase the glioma cell migration and invasion. The direct evidence is lacking on how collagen and hyaluronic acid in ECM affect glioma cell migration and proliferation. PURPOSE: In this work, we report the migration and cell cycle for glioma cells grown on collagen, or hyaluronic acid substrates. METHODS: Glioblastoma cells were grown on different substrates and labeled with Rhodamine Phalloidin to study the cell morphology. Flowcytometry was performed to study the cell cycle on different substrates. Time-lapse microscopy was used to study tumor cell motility. RESULTS: Our research found the different migration speeds of glioma cells on different substrates. Collagen surface promoted tumor cell migration. The tumor cells showed elongated shape on collagen substrate. Different glioma cell line showed different cell cycle behavior in the cell culture. CONCLUSION: Our study showed the impact of extracellular matrix components on cell proliferation and motility.Graduate School, Academic Affairs, University Librarie