Bioactive potential of Rosa canina L. fruits: antioxidant, cytotoxic, and antimicrobial activity with focus on antibiofilm properties

Abstract

R. canina fruits are well known bioactive agents. We have examined ethanolic and water extracts of fruits from Fruška gora, Serbia, using UHPL Q-ToF MS analysis, and by evaluating their flavonoid and phenolic content. Moreover, we have highlighted extracts antioxidant and cytotoxic potential and gave detailed insight into its antimicrobial potency. Both extracts were rich sources of numerous phytochemicals, phenolics and flavonoids. They have shown strong antioxidant potential and maintained non-cytotoxic towards HaCaT cell line. Their antibacterial potential was moderate (minimal inhibitory concentration 0.5 – 6 mg/mL), while their anticandidal potential was more pronounced (MIC 0.5 – 1 mg/mL). Ethanolic and water extract significantly affected the ability of C. albicans ATCC 10.231 to form biofilms. Moreover, 24 h old biofilms were significantly affected with minimal fungicidal concentration of extracts upon 120 min period. This study provided novel and more detailed information on the R. canina fruits bioactive potential, especially regarding its different aspects of antimicrobial activity. This natural product is rich source of phytochemicals and could be further explored especially in order to elucidate its antibiofilm properties