Bioluminescence Resonance Energy Transfer: A Powerful Technique in Neuropharmacology for Discovering New Ligands of G Protein-Coupled Receptors

Abstract

MasterThe Bioluminescence Resonance Energy Transfer (BRET) is a proximity-based assay used to study protein-protein interactions in living cells. The principle behind BRET involves resonance transfer of energy between bioluminescent enzymes and a fluorescent acceptors, resulting in measurable signals that indicate specific molecular interactions. BRET has been widely adopted in drug discovery as a high-throughput screening method to investigate the behavior of signaling proteins, such as G protein-coupled receptors (GPCRs). Due to its ability to operate under physiological conditions, BRET is a highly sensitive and well-suited tool for real-time, dynamic studies regarding diverse signaling pathways, their downstream messengers, receptor internalization and more